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HOME > Product search results > Code No. M162-3 Anti-p62 (SQSTM1) (Human) mAb

Code No. M162-3

Anti-p62 (SQSTM1) (Human) mAb

Price

¥48,000

Availability (in Japan)

10 or more

(In Japan at 00:05,
Nov 23, 2019 in JST)

Size

100 µg/100 µL

Data
  • Western Blotting

  • Immunoprecipitation

  • Flow Cytometry

  • Immunocytochemistry

  • Immunohistochemistry

Clonality Monoclonal Clone 5F2
Isotype (Immunized Animal) Mouse IgG1 κ
Applications
WB
1 µg/mL  
IP
2 µg/250 µL of cell extract from 2.5x106 cells  
FCM
2 µg/mL  
IC
5 µg/mL  
IH
2-10 µg/mL (Heat treatment is necessary for paraffin embedded sections.)  
Immunogen (Antigen) Recombinant Human p62 (120-440 a.a.)
Reactivity [Gene ID]

Human[8878], Mouse(-), Rat(-), Hamster(-)

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names sequestosome 1, p60, p62, A170, OSIL, PDB3, ZIP3, p62B
Background p62/SQSTM1 interacts with various molecular groups such as RIP, TRAF6, ERK, aPKCs, and poly-ubiquitin through PB1 domain, Zn finger domain, and UBA domain. This protein directly interacts with LC3, which is localized on autophagosome membrane, and is degradated by autophagic-lysosome pathway. p62 regulates ubiquitin-positive protein aggregates caused by autophagy deficiency.
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Citations

Western Blotting

  1. Soll C et al. Serotonin promotes tumor growth in human hepatocellular cancer. Hepatology 51, 1244-54 (2010)(PMID:20099302)
  2. Fan W et al. Keap1 facilitates p62-mediated ubiquitin aggregate clearance via autophagy. Autophagy 6, 614-21 (2010)(PMID:20495340)
  3. Wang BS et al. Autophagy negatively regulates cancer cell proliferation via selectively targeting VPRBP. Clin Sci (Lond). 124, 203-14 (2013)(PMID:22963397)
  4. Rovetta F et al. ER signaling regulation drives the switch between autophagy and apoptosis in NRK-52E cells exposed to cisplatin. Exp Cell Res. 318, 238-50 (2012)(PMID:22146761)
  5. Matsumoto G et al. Serine 403 phosphorylation of p62/SQSTM1 regulates selective autophagic clearance of ubiquitinated proteins. Mol Cell. 44, 279-89 (2011)(PMID:22017874)
  6. Schmeisser H et al. Type I interferons induce autophagy in certain human cancer cell lines. Autophagy 9, 683-96 (2013)(PMID:23419269)
  7. Guo X et al. Patulin induces pro-survival functions via autophagy inhibition and p62 accumulation. Cell Death Dis. 4, e822 (2013)(PMID:24091665)
  8. Zhu X et al. Cytosolic HMGB1 controls the cellular autophagy/apoptosis checkpoint during inflammation. J Clin Invest. 125, 1098-110 (2015)(PMID:25642769)
  9. Janda E et al. Parkinsonian toxin-induced oxidative stress inhibits basal autophagy in astrocytes via NQO2/quinone oxidoreductase 2: Implications for neuroprotection. Autophagy 11, 1063-80 (2015)(PMID:26046590)
  10. Stolz A et al. Fluorescence-based ATG8 sensors monitor localization and function of LC3/GABARAP proteins. EMBO J. 36, 549-564 (2017)(PMID:28028054)
  11. Sakamoto S et al. Mild MPP+ exposure-induced glucose starvation enhances autophagosome synthesis and impairs its degradation. Sci Rep. 7, 46668 (2017)(PMID:28443637)
  12. Wacker R et al. Fluorescence-based ATG8 sensors monitor localization and function of LC3/GABARAP proteins. Cell Microbiol. 19, e12754 (2017)(PMID:28573684)
  13. Bingel C et al. Three-dimensional tumor cell growth stimulates autophagic flux and recapitulates chemotherapy resistance. Cell Death Dis. 8, e3013 (2017)(PMID:28837150)
  14. Schmuckli-Maurer J et al. Inverted recruitment of autophagy proteins to the Plasmodium berghei parasitophorous vacuole membrane. PLoS One. 12, e0183797 (2017)(PMID:28841718)
  15. Lötzerich M et al. Rhinovirus 3C protease suppresses apoptosis and triggers caspase-independent cell death. Cell Death Dis. 9, 272 (2018)(PMID:29449668)
  16. Sun Y et al. 3′-epi-12β-hydroxyfroside, a new cardenolide, induces cytoprotective autophagy via blocking the Hsp90/Akt/mTOR axis in lung cancer cells. Theranostics 8, 2044-2060 (2018) (PMID:29556372)
  17. Kanda R et al. Expression of the glucagon-like peptide-1 receptor and its role in regulating autophagy in endometrial cancer. BMC Cancer 18, 657 (2018) (PMID:29907137)
  18. Qiu J et al. Distinct subgroup of the Ras family member 3 (DIRAS3) expression impairs metastasis and induces autophagy of gastric cancer cells in mice. J Cancer Res Clin Oncol. 144, 1869-1886 (2018)(PMID:30043279)
  19. Mori H et al. Induction of selective autophagy in cells replicating hepatitis C virus genome. J Gen Virol. 99, 1643-1657 (2018)(PMID:30311874)

Immunoprecipitation

  1. Wang BS et al. Autophagy negatively regulates cancer cell proliferation via selectively targeting VPRBP. Clin Sci (Lond). 124, 203-14 (2013)(PMID:22963397)

Immunocytochemistry

  1. Akaishi R et al. Autophagy in the placenta of women with hypertensive disorders in pregnancy. Placenta 35, 974-80 (2014)(PMID:25465707)
  2. Janda E et al. Parkinsonian toxin-induced oxidative stress inhibits basal autophagy in astrocytes via NQO2/quinone oxidoreductase 2: Implications for neuroprotection. Autophagy 11, 1063-80 (2015)(PMID:26046590)
  3. Schmuckli-Maurer J et al. Inverted recruitment of autophagy proteins to the Plasmodium berghei parasitophorous vacuole membrane. PLoS One. 12, e0183797 (2017)(PMID:28841718)
  4. Zhao Q et al. Dual Roles of Two Isoforms of Autophagy-related Gene ATG10 in HCV-Subgenomic replicon Mediated Autophagy Flux and Innate Immunity. Sci Rep. 7, 11250 (2017)(PMID:28900156)
  5. Doerflinger SY et al. Membrane alterations induced by nonstructural proteins of human norovirus. PLoS Pathog. 13, e1006705 (2017)(PMID:29077760)

Immunohistochemistry

  1. Wang BS et al. Autophagy negatively regulates cancer cell proliferation via selectively targeting VPRBP. Clin Sci (Lond). 124, 203-14 (2013)(PMID:22963397)
  2. Park JM et al. Prognostic impact of Beclin 1, p62/sequestosome 1 and LC3 protein expression in colon carcinomas from patients receiving 5-fluorouracil as adjuvant chemotherapy. Cancer Biol Ther. 14, 100-7 (2013)(PMID:23192274)
  3. Onodera Y et al. NRF2 immunolocalization in human breast cancer patients as a prognostic factor. Endocr Relat Cancer. 21, 241-52 (2014)
  4. Akaishi R et al. Autophagy in the placenta of women with hypertensive disorders in pregnancy. Placenta 35, 974-80 (2014)(PMID:25465707)
Product category
Research area
Autophagy
Brands
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  • The availability is based on the information in Japan at 00:05, Nov 23, 2019 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.