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HOME > Product search results > Code No. M186-3 Anti-LC3 mAb

Code No. M186-3

Anti-LC3 mAb



Special price


(ends 2020/03/31)


Availability (in Japan)

10 or more

(In Japan at 00:05,
Mar 30, 2020 in JST)


100 µg/100 µL

  • Western Blotting

Clonality Monoclonal Clone 8E10
Isotype (Immunized Animal) Mouse IgG2aκ
1 µg/mL  
reported.  (PMID: 28924380
Immunogen (Antigen) Human LC3, 1-120 aa Recombinant protein
Reactivity [Gene ID]

Human[81631], Mouse[67443], Rat[64862], Hamster[100769810]

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names microtubule-associated protein 1 light chain 3 beta, LC3B, ATG8F, MAP1LC3B-a, MAP1A/1BLC3, MAP1LC3B
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Notes Clone 8E10 reacts with LC3B and does not cross-react with LC3A, LC3C, GATE-16, and GABARAP.

Western Blotting

  1. Margariti A et al. XBP1 mRNA splicing triggers an autophagic response in endothelial cells through BECLIN-1 transcriptional activation. J Biol Chem 288, 859-72 (2013)(PMID:23184933)
  2. Mukai R et al. HTLV-1 HBZ positively regulates the mTOR signaling pathway via inhibition of GADD34 activity in the cytoplasm. Oncogene. 33, 2317-28 (2014)(PMID:23708656)
  3. Maejima Y et al. Mst1 inhibits autophagy by promoting the interaction between Beclin1 and Bcl-2. Nat Med. 19, 1478-88 (2013)(PMID:24141421)
  4. Wang M et al. EGFR-mediated chromatin condensation protects KRAS-mutant cancer cells against ionizing radiation. Cancer Res. 74, 2825-34 (2014)(PMID:24648348)
  5. He R et al. Divergent roles of BECN1 in LC3 lipidation and autophagosomal function. Autophagy 11, 740-7 (2015)(PMID:25955014)
  6. Hasegawa K et al. Promotion of mitochondrial biogenesis by necdin protects neurons against mitochondrial insults. Nat Commun. 7, 10943 (2016)(PMID:26971449)
  7. Li Z et al. Ammonia Induces Autophagy through Dopamine Receptor D3 and MTOR. PLoS One 11, e0153526 (2016)(PMID:27077655)
  8. Nakanishi T et al. Relationship between triterpenoid anticancer drug resistance, autophagy, and caspase-1 in adult T-cell leukemia. PeerJ 12, e2026 (2016)(PMID:27190722)
  9. Boada-Romero E et al. The T300A Crohn's disease risk polymorphism impairs function of the WD40 domain of ATG16L1. Nat Commun. 7, 11821 (2016)(PMID:27273576)
  10. Sasaki N et al. Chorein interacts with α-tubulin and histone deacetylase 6, and overexpression preserves cell viability during nutrient deprivation in human embryonic kidney 293 cells. FASEB J. 30, 3726-3732 (2016)(PMID:27468757)
  11. Maueröder C et al. Capability of Neutrophils to Form NETs Is Not Directly Influenced by a CMA-Targeting Peptide. Front Immunol. 8, 16 (2017)   (PMID:28191006)
  12. Zhao Z et al. Curcumin accelerates the repair of sciatic nerve injury in rats through reducing Schwann cells apoptosis and promoting myelinization. Front Immunol. 8, 16 (2017)(PMID:28622711)
  13. He R et al. Glucosyltransferase Activity of Clostridium difficile Toxin B Triggers Autophagy-mediated Cell Growth Arrest. Sci Rep. 7, 10532 (2017)(PMID:28874882)
  14. Sun Y et al. Dietary potassium regulates vascular calcification and arterial stiffness. JCI insight 2, e94920 (2017)(PMID:28978809)
  15. Wang H et al. Resveratrol attenuates oxidative damage through activating mitophagy in an in vitro model of Alzheimer's disease. Toxicol Lett. 282, 100-108 (2018)(PMID:29097221)
  16. Wang Y et al. AG-1031 induced autophagic cell death and apoptosis in C6 glioma cells associated with Notch-1 signaling pathway. J Cell Biochem. 119, 5893-5903(2018)(PMID:29575026)
  17. Li YC et al. Opposite Effects of Two Human ATG10 Isoforms on Replication of a HCV Sub-genomic Replicon Are Mediated via Regulating Autophagy Flux in Zebrafish. Front Cell Infect Microbiol. 8, 109 (2018)(PMID:29670865)
  18. Ito Y et al. Synergistic effects of HMG-CoA reductase inhibitor and angiotensin II receptor blocker on load-induced heart failure. FEBS Open Bio. 8, 799-816 (2018)(PMID:29670865)
  19. Taniguchi K et al. Depletion of gamma-glutamylcyclotransferase in cancer cells induces autophagy followed by cellular senescence. Am J Cancer Res. 8, 650-661 (2018)(PMID:29736310)
  20. Kozako T et al. Novel small molecule SIRT2 inhibitors induce cell death in leukemic cell lines. BMC Cancer. 18, 791 (2018)(PMID:30081901)
  21. Saito T et al. An alternative mitophagy pathway mediated by Rab9 protects the heart against ischemia. J Clin Invest. 129, 802-819 (2019) (PMID:30511961)
  22. Slowicka K et al. Physical and functional interaction between A20 and ATG16L1-WD40 domain in the control of intestinal homeostasis. Nat Commun. 10, 1834 (2019)(PMID:31015422)
  23. Kokubu Y et al. Phenotypic Drug Screening for Dysferlinopathy Using Patient-Derived Induced Pluripotent Stem Cells. Stem Cells Transl Med. 8, 1017-1029(2019)(PMID:31250983)


  1. Meng XH et al. Intracellular Insulin and Impaired Autophagy in a Zebrafish model and a Cell Model of Type 2 diabetes. Int J Biol Sci. 13, 985-995 (2017)(PMID:28924380)


  1. Li Z et al. Ammonia Induces Autophagy through Dopamine Receptor D3 and MTOR. PLoS One 11, e0153526 (2016)(PMID:27077655)
  2. Meng XH et al. Intracellular Insulin and Impaired Autophagy in a Zebrafish model and a Cell Model of Type 2 diabetes. Int J Biol Sci. 13, 985-995 (2017)(PMID:28924380)
  3. Li W et al. Autophagy is required for human umbilical cord mesenchymal stem cells to improve spatial working memory in APP/PS1 transgenic mouse model. Stem Cell Res Ther. 9, 9 (2018)(PMID:29335016)
Product category
Research area

  • The availability is based on the information in Japan at 00:05, Mar 30, 2020 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.