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CoralHue™ Basic fluorescent proteins


  • Brightly colored fluorescent proteins
  • Direct expression in cells without cytotoxicity

Diversity of Amalgaam’s basic fluorescent proteins

Amalgaam offers various fluorescent colors, from cyan to far-red, for labeling cells, subcellular structures and proteins, without cytotoxicity. Stable transformant cells and transgenic animals can also be established with our basic fluorescent proteins.



Products Name Color Excit./Emiss.
Maxima (nm)
Oligomerization *Brightness
KCy KCy1: Kusabira-Cyan Cyan 453/486 Dimer 16.9
Micy MiCy: Midoriishi-Cyan Cyan 472/495 Dimer 24.5
mMiCy: monomeric Midoriishi-Cyan Cyan 470/496 Monomer 15.5
UKG mUkG1: monomeric Umikinoko-Green1 Green 483/499 Monomer 43.2
AG AG: Azami-Green Green 492/505 Tetramer 48.4
mAG1: monomeric Azami-Green1 Green 492/505 Monomer 40.7
mAG407: monomeric Azami-Green407 Green 407/498 Monomer 13.6
KO KO1: Kusabira-Orange1 Orange 548/561 Dimer 33.2
mKO1: monomeric Kusabira-Orange1 Orange 548/559 Monomer 31.0
mKO2: monomeric Kusabira-Orange2 Orange 551/565 Monomer 39.6
dKeima dKeima570 Orange 440/570 Dimer 2.1
Keima-Red dKeima-Red Red 440/616 Dimer 7.6
mKeima-Red: monomeric Keima-Red Red 440/620 Monomer 3.5

*Brightness is shown by molar extinction coefficient x fluorescence quantum yield /1000.


CoralHue™ fluorescent protein vectors product codes

Abbr.
Form Excitation macima
(nm)
Emission maxima
(nm)
Vector
S1 MC1 MN1 MCLinker MNLinker
Kusabira-Cyan
KCy1 Dimer 453 486 AM-V0171M        
Midoriishi-Cyan
MiCy1 Dimer 472 495 AM-V0061M        
mMiCy1 Monomer 470 496 AM-V0111M        
hmMiCy1 Monomer 470 496   AM-V0115M AM-V0116M AM-V0119M AM-V0110M
Umikinoko-Green
mUKG1 Monomer 483 499 AM-V0161M        
hmUKG1 Monomer 483 499 AM-V0164M AM-V0165M AM-V0166M    
Azami-Green
AG Tetramer 492 505 AM-V0021M        
mAG1 Monomer 492 505 AM-V0031M AM-V0032M AM-V0033M    
hmAG1 Monomer 492 505 AM-V0034M AM-V0035M AM-V0036M AM-V0039M AM-V0030M
hmAG407 Monomer 407 498 AM-V0504M        
Kusabira-Orange
KO1 Dimer 548 561 AM-V0041M        
mKO1 Monomer 548 559 AM-V0051M AM-V0052M AM-V0053M    
mKO2 Monomer 551 565 AM-V0141M        
hKO1 Dimer 548 561 AM-V0044M AM-V0045M AM-V0046M    
hmKO2 Monomer 548 559 AM-V0054M AM-V0055M AM-V0056M AM-V0059M AM-V0050M
hmKO2 Monomer 551 565   AM-V0145M AM-V0146M AM-V0149M AM-V0140M
Keima570
dKeima570 Dimer 440 570 AM-V0121M        
hdKeima570 Dimer 440 570 AM-V0124M     AM-V0129M AM-V0120M
Keima-Red
dKeima-Red Dimer 440 616 AM-V0101M        
mKeima-Red Monomer 440 620 AM-V0091M   AM-V0093M    
hdKeima-Red Dimer 440 616 AM-V0104M     AM-V0109M AM-V0100M
hmKeima-Red Monomer 440 620 AM-V0094M     AM-V0099M AM-V0090M
Dronpa Green
DG1 Monomer 503 518 AM-V0071M AM-V0072M AM-V0073M    
hDG1 Monomer 503 518       AM-V0079M AM-V0070M
DG3 Monomer 491 514 AM-V0131M        
Kaede
Kaede Tetramer 508/572 518/580 AM-V0011M AM-V0012M AM-V0013M    
Kikume Green-Red
KikGR1 Tetramer 507/583 517/593 AM-V0081M AM-V0082M AM-V0083M    
mKikGR1 Monomer 505/580 517/591 AM-V0151M        
hKikGR1 Tetramer 507/583 517/593 AM-V0084M AM-V0085M AM-V0086M AM-V0089M AM-V0080M
hmKikGR1 Monomer 505/580 517/591       AM-V0159M AM-V0150M

S1: Vectors for subcloning
MC1, MN1: Expression vectors
Linker: Expression vectors with flexible linker (The linker sequence: TGNSADGGGGSGGSGGSGGGSTQG)
h: Humanized-codon
m: Monomer
d: Dimer

CoralHue fluorescent proteins were co-developed with the Laboratory for Cell Function and Dynamics, the Advanced Technology Development Center, the Brain Science Institute, RIKEN. MBL possesses the license.

Vector map

coralhue vector maps

CoralHue™ fluorescent proteins were co-developed with the Laboratory for Cell Function and Dynamics, the Advanced Technology Development Center, the Brain Science Institute, RIKEN. MBL possesses the license and deals in the products.


Please send us a completed and signed License Acknowledgement.

Please note that the product will be shipped after we receive a completed and signed License Acknowledgement (PDF file on the right). Prior to shipping, our staff may contact you to verify the intended use of the product. Please also note that a separate agreement or a contract may be required depending on the intended use.
The product you have ordered is sold for research purpose only. It is not intended for industrial or clinical use, and shall not be used for any purposes other than research. You are also asked not to hand over or resell the product to a third party.
If you belong to a profit organization or a business corporation, or wish to use the product for profit or commercial purposes, please contact us.


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