CoralHue™ Azami-Green (AG), cloned from the stony coral Galaxea fascicularis (Azami-sango in Japanese), absorbs light maximally at 492 nm and emits green light at 505 nm. AG is stable in the biological pH range and does not show a significant loss of fluorescent signal, making it advantageous over other fluorescent proteins such as EGFP. And AG also matures rapidly to form tetramers that are brighter than EGFP.
CoralHue™ monomeric Azami-Green (mAG1) maintains the brightness and pH stability of the parent protein AG. mAG1 can be used for labeling proteins or subcellular structures.
Azami Green(AG) spectrum data files (text files) | |
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AG excitation (![]() |
AG emission (![]() |
mAG1 excitation (![]() |
mAG1 emission (![]() |
Note: The file is in a tab-delimited text format. It contains values of the wavelength (0.5nm spacing) and brightness (fluorescence intensity peak value normalized to 1). Use a spreadsheet program to create a spectrum that will help you in choosing the appropriate excitation filter, dichroic mirror and fluorescence filter.
CHARACTERISTIC | AG | mAG1 |
---|---|---|
Oligomerization | Tetramer | Monomer |
Number of amino acid | 225 | 225 |
Excit./Emiss. Maxima (nm) | 492/505 | 492/505 |
Molar extinction coefficient (M-1cm-1) | 72,300 (492 nm) | 55,500 (492 nm) |
Fluorescence quantum yield | 0.67 | 0.74 |
Brightness *1 | 48.4 | 41.0 |
pH sensitivity | pKa<5.0 | pKa=5.8 |
Cytotoxicity *2 | No | No |
*1Brightness: Molar Extinction Coefficient ×Fluorescence Quantum Yield / 1000
*2Toxicity when expressed in HeLa cells
AG shows high resistance in a wide range of biological pH. This allows AG to be used identify cells or to report gene expression without being influenced by pH change. The pH dependence of both CoralHue™ AG and mAG1 was identical to that of its absorption spectra.
AG and mAG1 performance in fusions has been demonstrated in the nucleoplasm, plasma membrane, ER and mitochondria-targeting signal models. We offer these constructs as targeting vector series.
mAG1 performance in fusion has been demonstrated in p80 coilin, SC35 and fibrillarin.
CoralHue™ AG and mAG1 can be recognized using antibodies as shown below.
WB: Western blotting, IP: Immunoprecipitation, IC: Immunocytochemistry, IH: Immunohictochemistry
※CoralHue™ fluorescent proteins were co-developed with the Laboratory for Cell Function and Dynamics, the Advanced Technology Development Center, the Brain Science Institute, RIKEN. MBL possesses the license and deals in the products.
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