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HOME > Product search results > Code No. M135-3 Anti-GABARAP mAb

Code No. M135-3

Anti-GABARAP mAb

Price

¥48,000

Availability (in Japan)

10 or more

(In Japan at 00:05,
Nov 22, 2019 in JST)

Size

100 µg/100 µL

Data
  • Western Blotting

Clonality Monoclonal Clone 1F4
Isotype (Immunized Animal) Mouse IgG1
Applications
WB
1 µg/mL  
IC*
reported.  (PMID: 22948227
IH*
reported.  (PMID: 21684337
Immunogen (Antigen) Carrier protein conjugated human GABARAP N-terminal synthetic peptide
Reactivity [Gene ID]

Human[11337], Mouse[56486], Rat[58974], Hamster[100767236], Chicken*[107050619](PMID: 25514832)

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names GABA(A) receptor-associated protein, MM46, ATG8A, GABARAP-a
Background Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. LC3, GABARAP (γ-aminobutyric-acid-type-A-receptor-associated protein), and GATE-16 (Golgi-associated ATPase enhancer of 16 kDa) have been identified as a homologue of yeast Atg8. These homologues have been characterized as modifiers in reactions mediated by hAtg7 (an E1-like enzyme) and hAtg3 (an E2-like enzyme) as in yeast Atg8 lipidation. These homologues also generate form II, which are recovered in membrane fractions. Generation of the form II correlates with autophagosome association. These results suggest that all mammalian Atg8 homologues receive a common modification to associate with autophagosomal membrane as the form II.
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Citations

Western Blotting

  1. Tanji K et al. Alteration of autophagosomal proteins (LC3, GABARAP and GATE-16) in Lewy body disease. Neurobiol Dis. 43, 690-7 (2011)(PMID:21684337)
  2. Zhang Z et al. Gene deletion of Gabarap enhances Nlrp3 inflammasome-dependent inflammatory responses. J Immunol.190, 3517-24 (2013)(PMID:23427251)
  3. Maynard S et al. GABARAP is a determinant of apoptosis in growth-arrested chicken embryo fibroblasts. J Cell Physiol. 230, 1475-88 (2015)(PMID:25514832)
  4. Hervás JH et al. Human ATG3 binding to lipid bilayers: role of lipid geometry, and electric charge. Sci Rep. 7, 15614 (2017)(PMID:29142222)

Immunoprecipitation

  1. Joachim J et al. Centriolar Satellites Control GABARAP Ubiquitination and GABARAP-Mediated Autophagy. Curr Biol. 27, 2123-2136.e7 (2017)(PMID:28712572)

Immunocytochemistry

  1. Colecchia D et al. MAPK15/ERK8 stimulates autophagy by interacting with LC3 and GABARAP proteins. Autophagy 8, 1724-40 (2012)22948227(PMID:22948227)

Immunohistochemistry

  1. Tanji K et al. Alteration of autophagosomal proteins (LC3, GABARAP and GATE-16) in Lewy body disease. Neurobiol Dis. 43, 690-7 (2011)(PMID:21684337)
Product category
Research area
Autophagy
Brands
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  • The availability is based on the information in Japan at 00:05, Nov 22, 2019 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.