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HOME > Product search results > Code No. M152-3MS Anti-LC3 mAb for My select sampler set

Code No. M152-3MS

Anti-LC3 mAb for My select sampler set

Price

¥9,600

Availability (in Japan)

10 or more

(In Japan at 00:05,
Nov 14, 2019 in JST)

Size

40 µg/20 µL

Data
  • Western Blotting

  • Immunoprecipitation

Clonality Monoclonal Clone 4E12
Isotype (Immunized Animal) Mouse IgG1 κ
Applications
WB
5 µg/mL  
IP
5 µg/300 µL of cell extract from 1x107 cells  
FCM
40 µg/mL (final concentration)  
IC
40 µg/mL  
IH*
reported.  (PMID: 21957238 / 19955365 / 19915056
Other(Immuno-EM)
20 µg/mL  
Immunogen (Antigen) Recombinant Human LC3 (MAP1LC3B: 1-120 a.a.)
Reactivity [Gene ID]

Human[81631/ 84557], Mouse[67443/ 66734], Rat[64862/ 362245], Hamster[100760576/ 100769810]

Storage buffer 2 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names microtubule-associated protein 1 light chain 3 beta, microtubule-associated protein 1 light chain 3 alpha, LC3B, ATG8F, MAP1LC3B-a, MAP1A/1BLC3, MAP1LC3B, LC3A, ATG8E, MAP1ALC3, MAP1BLC3, MAP1LC3A
Background Macroautophagy mediates the bulk degradation of cytoplasmic components. These components are delivered to lysosomes via autophagosomes. The rat microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I. A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II. This antibody can detect both forms of LC3.
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Citations

Western Blotting

  1. Ho H et al. WIPI1 coordinates melanogenic gene transcription and melanosome formation via TORC1 inhibition. J Biol Chem. 286, 12509-23 (2011)(PMID:21317285)
  2. Saiki S et al. Caffeine induces apoptosis by enhancement of autophagy via PI3K/Akt/mTOR/p70S6K inhibition. Autophagy 7, 176-187 (2011)(PMID:21081844)
  3. Kasai M et al. Autophagic compartments gain access to the MHC class II compartments in thymic epithelium. J Immunol. 183, 7278-85 (2009)(PMID:19915056)
  4. Wang Y et al. Loss of macroautophagy promotes or prevents fibroblast apoptosis depending on the death stimulus. J Biol Chem. 283, 4766-77 (2008)(PMID:18073215)
  5. Murthy A et al. A Crohn's disease variant in Atg16l1 enhances its degradation by caspase 3. Nature 506, 456-62 (2014)(PMID:24553140)

Immunoprecipitation

  1. Mookerjee S et al. Posttranslational modification of ataxin-7 at lysine 257 prevents autophagy-mediated turnover of an N-terminal caspase-7 cleavage fragment. J Neurosci. 29, 15134-44 (2009)(PMID:19955365)

Immunocytochemistry

  1. Kasai M et al. Autophagic compartments gain access to the MHC class II compartments in thymic epithelium. J Immunol. 183, 7278-85 (2009)(PMID:19915056)
  2. Nishitsuji K et al. The E693Delta mutation in amyloid precursor protein increases intracellular accumulation of amyloid beta oligomers and causes endoplasmic reticulum stress-induced apoptosis in cultured cells. Am J Pathol. 174, 957-69 (2009)(PMID:19164507)
  3. Wang Y et al. Loss of macroautophagy promotes or prevents fibroblast apoptosis depending on the death stimulus. J Biol Chem. 283, 4766-77 (2008)(PMID:18073215)
  4. Chalazonitis A et al. Homeodomain interacting protein kinase 2 regulates postnatal development of enteric dopaminergic neurons and glia via BMP signaling. J Neurosci. 31, 13746-57 (2011)(PMID:21957238)
  5. Mori H et al. Induction of selective autophagy in cells replicating hepatitis C virus genome. J Gen Virol. 99, 1643-1657 (2018)(PMID:30311874)

Immunohistochemistry

  1. Kasai M et al. Autophagic compartments gain access to the MHC class II compartments in thymic epithelium. J Immunol. 183, 7278-85 (2009)(PMID:19915056)
  2. Mookerjee S et al. Posttranslational modification of ataxin-7 at lysine 257 prevents autophagy-mediated turnover of an N-terminal caspase-7 cleavage fragment. J Neurosci. 29, 15134-44 (2009)(PMID:19955365)

Other(Immuno-EM)

  1. Murthy A et al. A Crohn's disease variant in Atg16l1 enhances its degradation by caspase 3. Nature 506, 456-62 (2014)(PMID:24553140)
Product category
Research area
Autophagy
この製品に関するお問い合わせをする

  • The availability is based on the information in Japan at 00:05, Nov 14, 2019 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.