Anti-Atg7 (Human) pAb

Price

¥41,800

Availability (in Japan)

1-9

（In Japan at 17:00,
Mar 25, 2025 in JST）

Size

100 µL

Citations

Data Sheet

SDS

Data	Western Blotting Immunoprecipitation
Clonality	Polyclonal	Clone	Polyclonal
Isotype (Immunized Animal)	Rabbit Ig (aff.)
Applications	WB 1:1,000-1:2,000 IP 5 µL/300 µL of cell extract from 3x10⁶ cells
Immunogen (Antigen)	Human Atg7 C-terminal synthetic peptide
Reactivity [Gene ID]	Human[10533], Mouse(-), Rat(-), Hamster(-)
Storage buffer	PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	autophagy related 7, GSA7, APG7L, APG7-LIKE
Background	Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. Microtubule-associated protein light chain 3 (LC3) is a homologue of yeast Atg8, an essential component of autophagy. Following synthesis, the C-terminus of LC3 is cleaved by a cysteine protease-Atg4, to produce LC3-I, which is located in cytosolic fraction. LC3-I is activated by the E1-like enzyme Atg7 and forms a Atg7-LC3-I thioester. Atg7-LC3-I is transferred to Atg3 to form Atg3-LC3-I thioester. Atg3 is an E2-like enzyme that catalyzes the conjugation of LC3-I and phosphatidylethanolamine (PE) to form LC3-II. The LC3-II-PE conjugate is essential for binding tightly to autophagosomal membrane.
Related products	M133-3 Anti-Atg3 mAb PM050 Anti-Atg5 pAb M153-3 Anti-Atg5 mAb PM039 Anti-Atg7 (Human) pAb PM036 Anti-LC3 pAb M152-3 Anti-LC3 mAb PD042 Anti-Atg9A pAb M134-3 Anti-Atg4B mAb M154-3 Anti-Atg12 (Human) mAb PM040 Anti-Atg16L pAb M150-3 Anti-Atg16L mAb PM072 Anti-VMP1 pAb PM090 Anti-Atg8 (Filamentous fungi) pAb M152-3MS Anti-LC3 mAb for My select sampler set PM037 Anti-GABARAP pAb M135-3 Anti-GABARAP mAb M151-3 Anti-Atg10 (Human) mAb M186-3 Anti-LC3 mAb M200-3 Anti-NRF2 mAb PD014 Anti-LC3 pAb PM069 Anti-NRF2 pAb
Citations	Western Blotting Fujita K et al. Nrf2-mediated induction of p62 controls Toll-like receptor-4-driven aggresome-like induced structure formation and autophagic degradation. PNAS. 108, 1427-32 (2011)(PMID:21220332) Maejima Y et al. Mst1 inhibits autophagy by promoting the interaction between Beclin1 and Bcl-2. Nat Med. 19, 1478-88 (2013)(PMID:24141421) Horikawa I et al. Autophagic degradation of the inhibitory p53 isoform Δ133p53α as a regulatory mechanism for p53-mediated senescence. Nat Commun. 5, 4706 (2014)(PMID:25144556) Xu D et al. Modification of BECN1 by ISG15 plays a crucial role in autophagy regulation by type I IFN/interferon. Autophagy 11, 617-28 (2015)(PMID:25906440) He R et al. Divergent roles of BECN1 in LC3 lipidation and autophagosomal function. Autophagy 11, 740-7 (2015)(PMID:25955014) Dutta P et al. MIR494 reduces renal cancer cell survival coinciding with increased lipid droplets and mitochondrial changes. BMC Cancer 16, 33 (2016)(PMID:26794413) Kim HJ et al. Subcellular localization of FOXO3a as a potential biomarker of response to combined treatment with inhibitors of PI3K and autophagy in PIK3CA-mutant cancer cells. Oncotarget. 8, 6608-6622 (2017)(PMID:28036259) He R et al. Glucosyltransferase Activity of Clostridium difficile Toxin B Triggers Autophagy-mediated Cell Growth Arrest. Sci Rep. 7, 10532 (2017)(PMID:28874882) Liu WJ et al. Lysosome restoration to activate podocyte autophagy: a new therapeutic strategy for diabetic kidney disease. Cell Death Dis. 10, 806 (2019)(PMID:31649253) Chhabra R, Nanjundan M. Lysophosphatidic acid reverses Temsirolimus-induced changes in lipid droplets and mitochondrial networks in renal cancer cells. PLoS One.15, e0233887 (2020)(PMID:32492043) Immunofluorescence Liu WJ et al. Lysosome restoration to activate podocyte autophagy: a new therapeutic strategy for diabetic kidney disease. Cell Death Dis. 10, 806 (2019)(PMID:31649253)
Product category	Research area Autophagy

The availability is based on the information in Japan at 17:00, Mar 25, 2025 in JST.
The special price is shown in red color.
Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.