Anti-GABARAP pAb

Price

¥52,800

Availability (in Japan)

10 or more

（In Japan at 17:00,
Apr 19, 2024 in JST）

Size

100 µL

Citations

Data Sheet

SDS

This antibody reacts with GABARAP on Western blotting and Immunocytochemistry. It does not react with LC3 and GATE16.

Data	Western Blotting Immunocytochemistry
Clonality	Polyclonal	Clone	Polyclonal
Isotype (Immunized Animal)	Rabbit Ig (aff.)
Applications	WB 1:1,000 IC 1:100
Immunogen (Antigen)	Carrier protein conjugated GABARAP N-terminal synthetic peptide
Reactivity [Gene ID]	Human[11337], Mouse[56486], Rat[58974], Hamster[100767236]
Storage buffer	PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	GABA(A) receptor-associated protein, MM46, ATG8A, GABARAP-a
Background	Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. LC3, GAPARAP (γ-aminobutyric-acid-type-A-receptor-associated protein), and GATE-16 (Golgi-associated ATPase enhancer of 16 kDa) have been identified as a homologue of yeast Atg8. These homologues have been characterized as modifiers in reactions mediated by hAtg7 (an E1-like enzyme) and hAtg3 (an E2-like enzyme) as in yeast Atg8 lipidation. These homologues also generate form II, which are recovered in membrane fractions. Generation of the form II correlates with autophagosome association. These results suggest that all mammalian Atg8 homologues receive a common modification to associate with autophagosomal membrane as the form II.
Related products	M135-3 Anti-GABARAP mAb PM038 Anti-GATE-16 pAb PM072 Anti-VMP1 pAb PM036 Anti-LC3 pAb M152-3 Anti-LC3 mAb M186-3 Anti-LC3 mAb PD014 Anti-LC3 pAb PM045 Anti-p62 (SQSTM1) pAb M162-3 Anti-p62 (SQSTM1) (Human) mAb PM066 Anti-p62 C-terminal pAb M133-3 Anti-Atg3 mAb PM039 Anti-Atg7 (Human) pAb PM090 Anti-Atg8 (Filamentous fungi) pAb M152-3MS Anti-LC3 mAb for My select sampler set
Citations	Maruyama Y et al. LC3B is indispensable for selective autophagy of p62 but not basal autophagy. Biochem Biophys Res Commun. 446, 309-15 (2014)(PMID:24582747) Tanida I et al. In vitro assays of lipidation of Mammalian Atg8 homologs. Curr. Protoc. Cell Biol. 646, 11.20.1-13 (2014)(PMID:25181299) Szalai P et al. Autophagic bulk sequestration of cytosolic cargo is independent of LC3, but requires GABARAPs. Exp Cell Res. 333, 21-38 (2015)(PMID:25684710) Western Blotting Mariño G et al. Autophagy is essential for mouse sense of balance. J Clin Invest. 120, 2331-44 (2010)(PMID:20577052) Polletta L et al. SIRT5 regulation of ammonia-induced autophagy and mitophagy. Autophagy 11, 253-70 (2015)(PMID:25700560) Park S et al. O-GlcNAc modification is essential for the regulation of autophagy in Drosophila melanogaster. Cell Mol Life Sci. 72, 3173-83 (2015)(PMID:25840568) Winer H et al. Autophagy differentially regulates TNF receptor Fn14 by distinct mammalian Atg8 proteins. Nat Commun. 9, 3744 (2018)(PMID:30218067) Saladini S et al. Metformin Impairs Glutamine Metabolism and Autophagy in Tumour Cells. Cells 8, E49 (2019)(PMID:30646605) Qiao J et al. Identification of AaAtg8 as a marker of autophagy and a functional autophagy-related protein in Aedes albopictus. PeerJ. 6, e5988 (2018)(PMID:30498644) Saladini S et al. Metformin Impairs Glutamine Metabolism and Autophagy in Tumour Cells. Cells. 8, pii: E49 (2019) (PMID:30646605) Immunohistochemistry Li C et al. Spermine synthase deficiency causes lysosomal dysfunction and oxidative stress in models of Snyder-Robinson syndrome. Nat Commun. 8, 1257 (2017)(PMID:29097652)
Product category	Research area Autophagy

The availability is based on the information in Japan at 17:00, Apr 19, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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