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HOME > Product search results > Code No. PM037 Anti-GABARAP pAb

Code No. PM037

Anti-GABARAP pAb

Price

¥48,000

Availability (in Japan)

1-9

(In Japan at 00:05,
Nov 16, 2019 in JST)

Size

100 µL

This antibody reacts with GABARAP on Western blotting and Immunocytochemistry. It does not react with LC3 and GATE16.
Data
  • Western Blotting

  • Immunocytochemistry

Clonality Polyclonal Clone Polyclonal
Isotype (Immunized Animal) Rabbit Ig (aff.)
Applications
WB
1:1,000  
IC
1:100  
Immunogen (Antigen) Carrier protein conjugated GABARAP N-terminal synthetic peptide
Reactivity [Gene ID]

Human[11337], Mouse[56486], Rat[58974], Hamster[100767236]

Storage buffer PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names GABA(A) receptor-associated protein, MM46, ATG8A, GABARAP-a
Background Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. LC3, GAPARAP (γ-aminobutyric-acid-type-A-receptor-associated protein), and GATE-16 (Golgi-associated ATPase enhancer of 16 kDa) have been identified as a homologue of yeast Atg8. These homologues have been characterized as modifiers in reactions mediated by hAtg7 (an E1-like enzyme) and hAtg3 (an E2-like enzyme) as in yeast Atg8 lipidation. These homologues also generate form II, which are recovered in membrane fractions. Generation of the form II correlates with autophagosome association. These results suggest that all mammalian Atg8 homologues receive a common modification to associate with autophagosomal membrane as the form II.
Related products M135-3 Anti-GABARAP mAb
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PD014 Anti-LC3 pAb
PM045 Anti-p62 (SQSTM1) pAb
M162-3 Anti-p62 (SQSTM1) (Human) mAb
PM066 Anti-p62 C-terminal pAb
M133-3 Anti-Atg3 mAb
PM039 Anti-Atg7 (Human) pAb
PM090 Anti-Atg8 (Filamentous fungi) pAb
M152-3MS Anti-LC3 mAb for My select sampler set
Citations
  1. Maruyama Y et al. LC3B is indispensable for selective autophagy of p62 but not basal autophagy. Biochem Biophys Res Commun. 446, 309-15 (2014)(PMID:24582747)
  1. Tanida I et al. In vitro assays of lipidation of Mammalian Atg8 homologs. Curr. Protoc. Cell Biol. 646, 11.20.1-13 (2014)(PMID:25181299)
  1. Szalai P et al. Autophagic bulk sequestration of cytosolic cargo is independent of LC3, but requires GABARAPs. Exp Cell Res. 333, 21-38 (2015)(PMID:25684710)

Western Blotting

  1. Mariño G et al. Autophagy is essential for mouse sense of balance. J Clin Invest. 120, 2331-44 (2010)(PMID:20577052)
  2. Polletta L et al. SIRT5 regulation of ammonia-induced autophagy and mitophagy. Autophagy 11, 253-70 (2015)(PMID:25700560)
  3. Park S et al. O-GlcNAc modification is essential for the regulation of autophagy in Drosophila melanogaster. Cell Mol Life Sci. 72, 3173-83 (2015)(PMID:25840568)
  4. Winer H et al. Autophagy differentially regulates TNF receptor Fn14 by distinct mammalian Atg8 proteins. Nat Commun. 9, 3744 (2018)(PMID:30218067)
  5. Saladini S et al. Metformin Impairs Glutamine Metabolism and Autophagy in Tumour Cells. Cells 8, E49 (2019)(PMID:30646605)
  6. Qiao J et al. Identification of AaAtg8 as a marker of autophagy and a functional autophagy-related protein in Aedes albopictus. PeerJ. 6, e5988 (2018)(PMID:30498644)
  7. Saladini S et al. Metformin Impairs Glutamine Metabolism and Autophagy in Tumour Cells. Cells. 8, pii: E49 (2019) (PMID:30646605)

Immunohistochemistry

  1. Li C et al. Spermine synthase deficiency causes lysosomal dysfunction and oxidative stress in models of Snyder-Robinson syndrome. Nat Commun. 8, 1257 (2017)(PMID:29097652)
Product category
Research area
Autophagy
Brands
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  • The availability is based on the information in Japan at 00:05, Nov 16, 2019 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.