Anti-GATE-16 pAb

Price

¥52,800

Availability (in Japan)

10 or more

（In Japan at 17:00,
Mar 25, 2025 in JST）

Size

100 µL

Citations

Data Sheet

SDS

Data	Western Blotting Western Blotting
Clonality	Polyclonal	Clone	Polyclonal
Isotype (Immunized Animal)	Rabbit Ig (aff.)
Applications	WB 1:1,000 IH* reported （PMID: 21684337）
Immunogen (Antigen)	Carrier protein conjugated GATE-16 N-terminal synthetic peptide
Reactivity [Gene ID]	Human[11345], Mouse[93739], Rat[64670], Hamster[100767407]
Storage buffer	PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	GABARAPL2, GABA(A) receptor-associated protein-like 2, ATG8, GEF2, ATG8C, GEF-2, GATE16
Background	Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. LC3, GABARAP (Gamma-aminobutyric acid type A receptor-associated protein), and GATE-16 (Golgi-associated ATPase enhancer of 16 kDa) have been identified as a homologue of yeast Atg8. These homologues have been characterized as modifiers in reactions mediated by hAtg7 (an E1-like enzyme) and hAtg3 (an E2-like enzyme) as in yeast Atg8 lipidation. These homologues also generate form II, which are recovered in membrane fractions. Generation of the form II correlates with autophagosome association. These results suggest that all mammalian Atg8 homologues receive a common modification to associate with autophagosomal membrane as the form II.
Related products	PM037 Anti-GABARAP pAb M135-3 Anti-GABARAP mAb PM036 Anti-LC3 pAb M152-3 Anti-LC3 mAb M186-3 Anti-LC3 mAb PD014 Anti-LC3 pAb PM045 Anti-p62 (SQSTM1) pAb M162-3 Anti-p62 (SQSTM1) (Human) mAb PM066 Anti-p62 C-terminal pAb PM072 Anti-VMP1 pAb PM090 Anti-Atg8 (Filamentous fungi) pAb M152-3MS Anti-LC3 mAb for My select sampler set M133-3 Anti-Atg3 mAb
Notes	This antibody does not detect LC3 and GABARAP.
Citations	Western Blotting Fujita N et al. An Atg4B mutant hampers the lipidation of LC3 paralogues and causes defects in autophagosome closure. Mol Biol Cell 19, 4651-9 (2008)(PMID:18768752) Tanji K et al. Alteration of autophagosomal proteins (LC3, GABARAP and GATE-16) in Lewy body disease. Neurobiol Dis. 43, 690-7 (2011)(PMID:21684337) Niso-Santano M et al. Unsaturated fatty acids induce non-canonical autophagy. EMBO J. 34, 1025-41 (2015)(PMID:25586377) Polletta L et al. SIRT5 regulation of ammonia-induced autophagy and mitophagy. Autophagy 11, 253-70 (2015)(PMID:25700560) Skytte Rasmussen M et al. ATG4B contains a C-terminal LIR motif important for binding and efficient cleavage of mammalian orthologs of yeast Atg8. Autophagy 13, 834-853 (2017)(PMID:28287329) Winer H et al. Autophagy differentially regulates TNF receptor Fn14 by distinct mammalian Atg8 proteins. Nat Commun. 9, 3744 (2018)(PMID:30218067) Immunohistochemistry Tanji K et al. Alteration of autophagosomal proteins (LC3, GABARAP and GATE-16) in Lewy body disease. Neurobiol Dis. 43, 690-7 (2011)(PMID:21684337)
Product category	Research area Autophagy

The availability is based on the information in Japan at 17:00, Mar 25, 2025 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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