Anti-Phospho-p62 (SQSTM1) (Ser403) mAb

Price

¥52,800

Availability (in Japan)

10 or more

（In Japan at 00:05,
Mar 23, 2025 in JST）

Size

100 µL (1 mg/mL)

Data Sheet

SDS

Data	Western Blotting Immunohistochemistry
Clonality	Monoclonal	Clone	4C8
Isotype (Immunized Animal)	Rat IgG2aκ
Applications	WB 5 µg/mL （PMID: 22017874） IH 5 µg/mL （PMID: 22017874）
Immunogen (Antigen)	Synthetic peptide CKKKESLSQMLpSMGFSDEGKKK (corresponding to amino acid residues 396-410 of human p62 (SQSTM1))
Reactivity [Gene ID]	Human[8878], Mouse[18412]
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	sequestosome-1, A170, OSIL, PDB3, ZIP3
Background	p62/SQSTM1 is a major adaptor protein for selective autophagy. S403 is in a highly conserved ubiquitin association (UBA) domain of p62 and is directly phosphorylated by CK2 or TBK1. The S403 phosphorylation of p62 results in the enhanced binding affinity between p62 and polyubiquitin chain and induces its autophagic degradation. S403 phosphorylated p62 is concentrated in autophagosomes that is accumulated by Bafilomycin A1 treatment and in ubiquitin positive protein aggregates in autophagy deficient cells.
Related products	D343-3 Anti-Phospho-p62 (SQSTM1) (Ser403) mAb PM045 Anti-p62 (SQSTM1) pAb M162-3 Anti-p62 (SQSTM1) (Human) mAb PM066 Anti-p62 C-terminal pAb M162-A48 Anti-p62 (SQSTM1) (Human) mAb-Alexa Fluor™ 488 M162-A59 Anti-p62 (SQSTM1) (Human) mAb-Alexa Fluor™ 594 M162-A64 Anti-p62 (SQSTM1) (Human) mAb-Alexa Fluor™ 647 8485 Autophagy Ab Sampler Set PM074 Anti-Phospho-p62 (SQSTM1) (Ser351) pAb D343-3MS Anti-Phospho-p62 (SQSTM1) (Ser403) mAb for My select sampler set PM074MS Anti-Phospho-p62 (SQSTM1) (Ser351) pAb for My select sampler set M230-3 Anti-Parkin mAb 8486 Autophagy Watch M217-3 Anti-Phospho-p62 (SQSTM1) (Ser351) mAb M217-3MS Anti-Phospho-p62 (SQSTM1) (Ser351) mAb for My select sampler set M224-3 Anti-KEAP1 mAb CY-7055 CycLex^® Total p62 ELISA Kit
Notes	Clone 4C8 recognizes S403-phosphoryalted p62, but is different from clone 4F6.
References	Matsumoto G et al. Serine 403 phosphorylation of p62/SQSTM1 regulates selective autophagic clearance of ubiquitinated proteins. Mol Cell. 44, 279-89 (2011)（PMID: 22017874）
Product category	Research area Autophagy

The availability is based on the information in Japan at 00:05, Mar 23, 2025 in JST.
The special price is shown in red color.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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