- Bright green fluorescence
- FRET donor with high pH stability
The fluorescent protein Umikinoko-Green (UkG) gene was isolated from the soft coral Sarcophyton sp. (umi-kinoko in Japanese).
UkG rapidly matures to form a dimeric complex. CoralHue™ monomeric Umikinoko-Green (mUkG1) maintains the brightness and pH stability of parent protein UkG. mUkG1 absorbs light maximally at 483 nm and emits green light at 499 nm. mUkG1 exhibits brilliant fluorescence and has extremely high pH stability. mUkG1 can be used to label proteins, subcellular structures or for FRET analysis.
Note: The file is in a tab-delimited text format. It contains values of the wavelength (0.5nm spacing) and brightness (fluorescence intensity peak value normalized to 1). Use a spreadsheet program to create a spectrum that will help you in choosing the appropriate excitation filter, dichroic mirror and fluorescence filter.
|Number of amino acid
|Excit./Emiss. maxima (nm)
|Molar extinction coefficient (M-1cm-1)
||60,000 (483 nm)
|Fluorescence quantum yield
*1Brightness: Molar Extinction Coefficient ×Fluorescence Quantum Yield / 1000
*2Toxicity when expressed in HeLa cells
Voltage sensor “Mermaid”
Mermaid is a membrane potential reporter using FRET. The structure of Mermaid consisting of the VSD of Ci-VSP and mUkG1-mKOκ in tandem. mUKG1 and mKO-κ are good FRET partners of donor and acceptor. mUKG's emission and mKO-κ's absorption greatly overlapped with a calculated Förster distance of 5.5 nm.
- Tsutsui H, Karasawa S, Okamura Y, Miyawaki A. Improving membrane voltage measurements using FRET with new fluorescent proteins.; Nat Methods., 5, 683-685(2008) PMID:18622396
※CoralHue™ fluorescent proteins were co-developed with the Laboratory
for Cell Function and Dynamics, the Advanced Technology Development
Center, the Brain Science Institute, RIKEN. MBL possesses the license
and deals in the products.
Please send us a completed and signed License Acknowledgement.
Please note that the product will be shipped after we receive a
completed and signed License Acknowledgement (PDF file on the right).
Prior to shipping, our staff may contact you to verify the intended use
of the product. Please also note that a separate agreement or a contract
may be required depending on the intended use.
The product you have ordered is sold for research purpose only. It is
not intended for industrial or clinical use, and shall not be used for
any purposes other than research. You are also asked not to hand over or
resell the product to a third party.
If you belong to a profit organization or a business corporation, or
wish to use the product for profit or commercial purposes, please