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Organelle targeting vectors


  • Tools for labeling of subcellular structure
  • Multi-labeling cells with different colors

CoralHue ® Organelle targeting vectors allowing to visualize subcellular structures. These vectors encode fusions of fluorescent protein variants and localization signals. It can be used for fluorescent labeling of subcellular structure in living cells.



Plasma membrane (PM)

  

Mitochondria (MT)

  

Endoplasmic reticulum (ER)

  

Nucleoplasm (NP)

  



Applications

Dual-labeling cells with CoralHue® fluorescent proteins



Multi-labeling cell with CoralHue® fluorescent proteins



Table: CoralHue® Organelle Targeting Vectors
Targeted
subcellular structure
Color
variations
Fluorescence
proteins
Localization tag
(Targeting sequence)
Localization Potential
applications
Plasma Membrane(PM)
Green
Orange
Red
Azami-Green(mAG1)
Kusabira-Orange(mKO1)
Keima-Red(mKeima-Red)
Lyn A Protein tyrosine
kinase
[Rattus norvegicus]
Inner plasma membrane ・Track membrane dynamics.
・Monitor membrane-associated
Mitochondria(MT) Green
Orange
Red
Azami-Green (mAG1)
Kusabira-Orange(mKO1)
Keima-Red(mKeima-Red)
Cytochrome c oxidase
polypeptide IV
[Saccharomyces
Mitochondrial inner
membrane
・Track mitochondria dynamics.
Endoplasmic reticulum(ER) Green
Orange
Azami-Green(mAG1)
Kusabira-Orange(mKO1)
Calreticulin;KDEL retrieval
sequence[Bos taurus]
Lumen of the endoplasmic reticulum ・Track morphology and intracellular distribution.
Nucleoplasm(NP) Green
Orange
Red
Azami-Green(AG)
Kusabira-Orange(KO1)
Keima-Red(hdKeima-Red)
Poly(ADP-ribose)
Polymerase
(PARP)[Homosapiens]
Intranuclear ・Track cell lineage.
・Monitor cell growth and division.


※CoralHue® fluorescent proteins were co-developed with the Laboratory for Cell Function and Dynamics, the Advanced Technology Development Center, the Brain Science Institute, RIKEN. MBL possesses the license and deals in the products.


Please send us a completed and signed License Acknowledgement.

Please note that the product will be shipped after we receive a completed and signed License Acknowledgement (PDF file on the right). Prior to shipping, our staff may contact you to verify the intended use of the product. Please also note that a separate agreement or a contract may be required depending on the intended use.
The product you have ordered is sold for research purpose only. It is not intended for industrial or clinical use, and shall not be used for any purposes other than research. You are also asked not to hand over or resell the product to a third party.
If you belong to a profit organization or a business corporation, or wish to use the product for profit or commercial purposes, please contact us.