- FRET donor with highly fluorescent quantum yield
- Cell, subcellular structure, or protein labeling
The fluorescent protein Midoriishi-Cyan gene was isolated from the
stony coral Acropora sp.(Midori-ishi in Japanese).
CoralHue™ Midoriishi-Cyan 1 (MiCy1) absorbs light maximally at 472 nm
and emits cyan light at 495 nm. It rapidly matures to form a bright dimeric
complex. So it can be used to mark individual cells or to report gene
expression without the problem of protein aggregation. CoralHue™
mMiCy1 is a monomeric version of MiCy1, and can be used to label
proteins or subcellular structures.
These fluorescent proteins are suitable as donor for fluorescence
resonance energy transfer (FRET) because of highly fluorescent
Expressiom of Midoriishi-Cyan in HeLa cells
Note: The file is in a tab-delimited text format. It contains values of the wavelength (0.5nm spacing) and brightness (fluorescence intensity peak value normalized to 1). Use a spreadsheet program to create a spectrum that will help you in choosing the appropriate excitation filter, dichroic mirror and fluorescence filter.
※CoralHue™ fluorescent proteins were co-developed with the Laboratory for Cell Function and Dynamics, the Advanced Technology Development Center, the Brain Science Institute, RIKEN. MBL possesses the license and deals in the products.