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HOME > Product category > Fluorescent proteins > Kusabira-Orange

Kusabira-Orange


  • Bright orange fluorescence
  • High pH stability
  • Monomer/dimer

CoralHue® Kusabira-Orange (KO1), from the stony coral Fungia concinna (Kusabira-ishi in Japanese), absorbs light maximally at 548 nm and emits orange light at 561 nm. KO1 rapidly matures to form a fluorescent dimeric complex. KO1 can be used to mark cells or to report gene expression without problems stemming from protein aggregation.

CoralHue® monomeric Kusabira Orange (mKO1) maintains the brilliance and pH stability of the parent protein. mKO1 can be used to label proteins or subcellular structures or for FRET analysis.

CoralHue® mKO2 is a mutant of mKO1 that features rapid maturation. mKO2 can be used to label proteins or subcellular structures or for reporter assays.

Kusabira-Orange: KO images 


Fluorescent properties

KO1


mKO1

fluorescent properties

mKO2

fluorescent properties

Kusabira-Orange1 spectrum data files (text files)
Kusabira-Orange1 excitation (8K)  Kusabira-Orange1 emission (8K)
mKusabira-Orange1 excitation (8K)  mKusabira-Orange1 emission (8K)
mKusabira-Orange2 excitation (4K)  mKusabira-Orange2 emission (4K)

Note: The file is in a tab-delimited text format. It contains values of the wavelength (0.5nm spacing) and brightness (fluorescence intensity peak value normalized to 1). Use a spreadsheet program to create a spectrum that will help you in choosing the appropriate excitation filter, dichroic mirror and fluorescence filter.

CHARACTERISTIC KO1 mKO1 mKO2
Oligomerization Dimer Monomer Monomer
Number of amino acid 218 218 218
Excit./Emiss. maxima (nm) 548/561 548/559 551/565
Molar Extinction Coefficient (M-1cm-1) 73,700 (548 nm) 51,600 (548 nm) 63,800 (551 nm)
Fluorescence quantum yield 0.45 0.60 0.62
Brightness*1 33.2 31.0 39.6
pH sensitivity pKa<5.0 pKa<5.0 pKa=5.5
Cytotoxicity*2 Not observed Not observed Not observed
Resistance to PFA fixation Not tested Not tested +++

*1Brightness: Molar Extinction Coefficient ×Fluorescence Quantum Yield / 1000
*2Toxicity when expressed in HeLa cells

 

Performance and use

Kusabira-Orange are useful for labeling organelle.

Kusabira-Orange is easily expressed and detected in a wide range of organisms. It has been demonstrated in nucleoplasm, plasma membrane, endoplasmic reticulum and mitochondrial targeting signal models.


Kusabira-Orange is a useful protein for expression into various species.

AG resistant to acidity AG resistant to acidity AG resistant to acidity AG resistant to acidity

KO1 TG pig

Provided by Dr. Nagashima
Meiji University
Cloning and Stem Cells. (2008) 10: 313-324.

mKO1 fused with Vasa protein expressed in Drosophila ovary (red).

Provided by Dr. Nakamura RIKEN

The olfactory bulb of zebrafish

Provided by Dr. Miyasaka and Dr.
Yoshihara
Laboratory for Neurobiology of Synapse, BSI, RIKEN

KO1 expressed in the epidermis of onion

Provided by Dr. Iida and Dr. Hoshino
the National Institute of
Basic Biology (NIBB).

Recommended antibodies

CoralHue® KO1, mKO1 and mKO2 can be recognized using antibodies as shown below.

  • Anti-monomeric Kusabira-Orange 1 for WB, Code No. M104-3M
  • Anti-monomeric Kusabira-Orange 1 for IP, Code No. M105-3M
  • Anti-monomeric Kusabira-Orange 2 for WB, IP, IC and IH, Code No. M168-3M
  • Anti-monomeric Kusabira-Orange 2 for WB, IP, IC and IH, Code No. PM051M

WB: Western blotting, IP: Immunoprecipitation, IC: Immunocytochemistry, IH: Immunohistochemistry


References

  • Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A, Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer; Biochem J. 381, 307-12(2004) PMID: 15065984
  • Matsunari H, Onodera M, Tada N, Mochizuki H, Karasawa S, Haruyama E, Nakayama N, Saito H, Ueno S, Kurome M, Miyawaki A, Nagashima H., Transgenic-Cloned Pigs Systemically Expressing Red Fluorescent Protein, Kusabira-Orange; Cloning and Stem Cells. (2008) 10: 313-324. PMID: 18729767

CoralHue® fluorescent proteins were co-developed with the Laboratory for Cell Function and Dynamics, the Advanced Technology Development Center, the Brain Science Institute, RIKEN. MBL possesses the license and deals in the products.


Please send us a completed and signed License Acknowledgement.

Please note that the product will be shipped after we receive a completed and signed License Acknowledgement (PDF file on the right). Prior to shipping, our staff may contact you to verify the intended use of the product. Please also note that a separate agreement or a contract may be required depending on the intended use.
The product you have ordered is sold for research purpose only. It is not intended for industrial or clinical use, and shall not be used for any purposes other than research. You are also asked not to hand over or resell the product to a third party.
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