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HOME > Product search results > Code No. M225-3 Anti-NFIL3 (E4BP4) chimeric mAb

Code No. M225-3

Anti-NFIL3 (E4BP4) chimeric mAb



Availability (in Japan)

10 or more

(In Japan at 17:00,
Jul 1, 2022 in JST)


100 µg/100 µL

This antibody is a chimeric antibody composed of guinea pig Ig κ and rabbit Fc. Please use anti-rabbit IgG antibody as a secondary antibody.
  • Western Blotting

  • Western Blotting

  • Immunoprecipitation

  • Immunoprecipitation

  • Flow Cytometry

  • Immunohistochemistry

Clonality Monoclonal Clone 42
Isotype (Immunized Animal) Guinea pig Ig κ (chimeric)
1 -2 µg/mL  
1 -2 µg/sample  
1 µg/mL  
Can be used.  
Immunogen (Antigen) Recombinant protein, corresponding to internal region of mouse Nfil3 (E4bp4)
Reactivity [Gene ID]

Human[4783], Mouse[18030], Rat(-)

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names IL3BP1, NFIL3A, NF-IL3A
Background NFIL3, or E4BP4, is a transcription factor that controls sleep and circadian rhythms throughout the cranial nerve, endocrine, and metabolic systems.The circadian clock is a 24-hour endogenous oscillator, which cycles via transcriptional/translational feedback loops of clock genes and their products in individual cells. CLOCK and BMAL1 are positive factors in the loop, and their complex binds to E-box elements of PERs and CRYs. PER and CRY bind the CLOCK-BMAL1 complex, suppressing E-box-dependent transactivation and forming a molecular clock via this negative feedback mechanism.D-box and REV-ERB/ROR-binding elements also form a regulatory network of clock-controlled genes (CCGs). D-box-dependent transactivation is activated by DBP and repressed by NFIL3/E4BP4.NFIL3/E4BP4 expression also plays an important role in the immune system. NFIL3 polymorphisms are associated with inflammatory bowel disease (IBD).
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D334-3 Anti-CLOCK (Mouse) mAb
D333-3 Anti-CLOCK (Mouse) mAb
PM079 Anti-DBP (Mouse) pAb
PM075 Anti-GNAT2 (Zebrafish) pAb
PM081 Anti-Cry1 (Mouse) pAb
PM082 Anti-Cry2 (Mouse) pAb
PM083 Anti-Per2 (Mouse) pAb
PM087 Anti-Chrono (Mouse) pAb
PM091 Anti-Per1 (Mouse) pAb
PM092 Anti-NR1D1 (Rev-erbα) pAb
PM093 Anti-NR1D2 (Rev-erbβ) pAb
D349-3 Anti-CLOCK mAb
M219-3 Anti-RORγt mAb
PM096 Anti-PER2 (Human) pAb
PM097 Anti-NFIL3 (E4BP4) pAb
Notes *Zeitgeber time: Mice were maintained in a light-dark (LD) cycle (12 hr light, 12 hr dark with lights on time was designatedas Zeitgeber time (ZT) 0 and light off time was designated as ZT12).

Western Blotting

  1. Yu F et al. The Circadian Clock Gene Bmal1 Controls Intestinal Exporter MRP2 and Drug Disposition. Theranostics. 9, 2754-2767 (2019)(PMID:31244920)
  1. Yu X et al. Th17 cell differentiation is regulated by the circadian clock. Science. 342, 727-30 (2013)(PMID: 24202171
  2. Firth MA et al. Nfil3-independent lineage maintenance and antiviral response of natural killer cells. J Exp Med. 210, 2981-90 (2013)
     (PMID: 24277151
  3. Kashiwada M et al. IL-4-induced transcription factor NFIL3/E4BP4 controls IgE class switching. PNAS 107, 821-6 (2010)(PMID: 20080759
  4. Mitsui S et al. Antagonistic role of E4BP4 and PAR proteins in the circadian oscillatory mechanism. Genes Dev. 15, 995-1006 (2001)(PMID: 11316793
  5. Ikushima S et al. Pivotal role for the NFIL3/E4BP4 transcription factor in interleukin 3-mediated survival of pro-B lymphocytes. PNAS 94, 2609-14 (1997)(PMID: 9122243
  6. Zhang W et al. Molecular cloning and characterization of NF-IL3A, a transcriptional activator of the human interleukin-3 promoter. Mol Cell Biol. 15, 6055–63 (1995)(PMID: 7565758
  7. Cowell IG et al. Transcriptional repression by a novel member of the bZIP family of transcription factors. Mol Cell Biol. 12, 3070-7 (1992)(PMID: 1620116
Product category
Research area
Circadian rhythm

  • The availability is based on the information in Japan at 17:00, Jul 1, 2022 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.