Data |
Western Blotting

Western Blotting

Western Blotting

Immunoprecipitation

|
Clonality |
Polyclonal
|
Clone |
Polyclonal |
Isotype
(Immunized Animal)
|
Rabbit Ig (aff.)
|
Applications |
- WB
- 1:200-1:1,000
 - IP
- 2 µL/sample
|
Reactivity
[Gene ID] |
Human[9572], Mouse[217166], Rat[252917] |
Storage buffer |
PBS/50% glycerol, pH 7.2
|
Storage temp. |
-20°C
|
Conjugate |
Unlabeled |
Manufacturer |
MBL |
Alternative names |
Nuclear receptor subfamily 1 group D member 1, Rev-erbA-alpha, V-erbA-related protein 1, Ear1, EAR1, THRA1,THRAL
|
Related products |
D335-3 Anti-BMAL1 mAb D334-3 Anti-CLOCK (Mouse) mAb D333-3 Anti-CLOCK (Mouse) mAb PM079 Anti-DBP (Mouse) pAb PM075 Anti-GNAT2 (Zebrafish) pAb D349-3 Anti-CLOCK mAb PM081 Anti-Cry1 (Mouse) pAb PM082 Anti-Cry2 (Mouse) pAb PM083 Anti-Per2 (Mouse) pAb PM087 Anti-Chrono (Mouse) pAb PM091 Anti-Per1 (Mouse) pAb PM093 Anti-NR1D2 (Rev-erbβ) pAb PM096 Anti-PER2 (Human) pAb PM097 Anti-NFIL3 (E4BP4) pAb
|
Notes |
*Zeitgeber time: Mice were maintained in a light-dark (LD) cycle (12 hr light, 12 hr dark with lights on time was designatedas Zeitgeber time (ZT) 0 and light off time was designated as ZT12).
|
Citations |
Western Blotting - Imamura K et al. ASK family kinases mediate cellular stress and redox signaling to circadian clock. PNAS 115, 3646-3651 (2018)(PMID:29555767)
- Hiramoto K et al. An Inhibitor of Casein Kinase 1ε/δ (PF670462) Prevents the Deterioration of Dextran Sodium Sulfate-induced Ulcerative Colitis Caused by UVB Eye Irradiation. Int J Biol Sci. 14, 992-999 (2018)(PMID:29989105)
- Masuda S et al. Mutation of a PER2 phosphodegron perturbs the circadian phosphoswitch. Proc Natl Acad Sci U S A. 117, 10888-10896 (2020)(PMID:32354999)
|
References |
- Preitner N et al. The orphan nuclear receptor REV-ERBalpha controls circadian transcription within the positive limb of the mammalian circadian oscillator. Cell 110, 251–60 (2002)(PMID: 12150932)
- Triqueneaux G et al. The orphan receptor Rev-erbalpha gene is a target of the circadian clock pacemaker. J Mol Endocrinol. 33, 585–608 (2004)(PMID: 15591021)
- Raghuram S et al. Identification of heme as the ligand for the orphan nuclear receptors REV-ERBalpha and REV-ERBbeta. Nat Struct Mol Biol. 14, 1207–13 (2007)(PMID: 18037887)
- Liu AC et al. Redundant function of REV-ERBalpha and beta and non-essential role for Bmal1 cycling in transcriptional regulation of intracellular circadian rhythms. PLoS Genet. 4, e1000023 (2008)(PMID: 18454201)
- Bugge A et al. Rev-erbα and Rev-erbβ coordinately protect the circadian clock and normal metabolic function. Genes Dev. 26, 657–67 (2012)(PMID: 22474260)
|
Product category |
- Research area
- Circadian rhythm
Signal transduction
|