Code No. | M225-3 | Print out |
Anti-NFIL3 (E4BP4) chimeric mAb | ||
This antibody is a chimeric antibody composed of guinea pig Ig κ and rabbit Fc. Please use anti-rabbit IgG antibody as a secondary antibody. | ||
Price | ¥48,000 | |
Size | 100 µL (1 mg/mL) | |
Availability (in Japan) | 10 or more
(In Japan at 17:00, Mar 28, 2024 in JST) |
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Clonality | Monoclonal | |
Clone | 42 | |
Isotype (Immunized Animal) |
Guinea pig Ig κ (chimeric) | |
Applications | WB 1 -2 µg/mL IP 1 -2 µg/sample FCM 1 µg/mL IH Can be used. |
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Immunogen (Antigen) |
Recombinant protein, corresponding to internal region of mouse Nfil3 (E4bp4) | |
Reactivity [Gene ID] | ||
Storage buffer | 1 mg/mL in PBS/50% glycerol, pH 7.2 | |
Storage temp. | -20°C | |
Conjugate | Unlabeled | |
Manufacturer | MBL | |
Alternative names | IL3BP1, NFIL3A, NF-IL3A | |
Background | NFIL3, or E4BP4, is a transcription factor that controls sleep and circadian rhythms throughout the cranial nerve, endocrine, and metabolic systems.The circadian clock is a 24-hour endogenous oscillator, which cycles via transcriptional/translational feedback loops of clock genes and their products in individual cells. CLOCK and BMAL1 are positive factors in the loop, and their complex binds to E-box elements of PERs and CRYs. PER and CRY bind the CLOCK-BMAL1 complex, suppressing E-box-dependent transactivation and forming a molecular clock via this negative feedback mechanism.D-box and REV-ERB/ROR-binding elements also form a regulatory network of clock-controlled genes (CCGs). D-box-dependent transactivation is activated by DBP and repressed by NFIL3/E4BP4.NFIL3/E4BP4 expression also plays an important role in the immune system. NFIL3 polymorphisms are associated with inflammatory bowel disease (IBD). | |
Related products | D335-3 Anti-BMAL1 mAb D334-3 Anti-CLOCK (Mouse) mAb D333-3 Anti-CLOCK (Mouse) mAb PM079 Anti-DBP (Mouse) pAb PM075 Anti-GNAT2 (Zebrafish) pAb PM081 Anti-Cry1 (Mouse) pAb PM082 Anti-Cry2 (Mouse) pAb PM083 Anti-Per2 (Mouse) pAb PM087 Anti-Chrono (Mouse) pAb PM091 Anti-Per1 (Mouse) pAb PM092 Anti-NR1D1 (Rev-erbα) pAb PM093 Anti-NR1D2 (Rev-erbβ) pAb D349-3 Anti-CLOCK mAb M219-3 Anti-RORγt mAb PM096 Anti-PER2 (Human) pAb PM097 Anti-NFIL3 (E4BP4) pAb |
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Notes | *Zeitgeber time: Mice were maintained in a light-dark (LD) cycle (12 hr light, 12 hr dark with lights on time was designatedas Zeitgeber time (ZT) 0 and light off time was designated as ZT12). | |
Product category | Research area:Immunology Circadian rhythm | |
Data | ||
Citations | Western Blotting
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References |
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