Anti-GABARAP pAb

価格（税別）

¥52,800

在庫

10以上

（2024/04/18 17:00時点）

包装

100 µL

使用文献

データシート

SDS

This antibody reacts with GABARAP on Western blotting and Immunocytochemistry. It does not react with LC3 and GATE16.

データ	Western Blotting Immunocytochemistry
クローナリティー	Polyclonal	クローン	Polyclonal
アイソタイプ（免疫動物）	Rabbit Ig (aff.)
使用法	WB 1:1,000 IC 1:100
免疫原(抗原)	Carrier protein conjugated GABARAP N-terminal synthetic peptide
交差性 [Gene ID]	Human[11337], Mouse[56486], Rat[58974], Hamster[100767236]
性状	PBS/50% glycerol, pH 7.2
保存温度	-20°C	標識	Unlabeled	メーカー	MBL
別称	GABA(A) receptor-associated protein, MM46, ATG8A, GABARAP-a
背景	Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. LC3, GAPARAP (γ-aminobutyric-acid-type-A-receptor-associated protein), and GATE-16 (Golgi-associated ATPase enhancer of 16 kDa) have been identified as a homologue of yeast Atg8. These homologues have been characterized as modifiers in reactions mediated by hAtg7 (an E1-like enzyme) and hAtg3 (an E2-like enzyme) as in yeast Atg8 lipidation. These homologues also generate form II, which are recovered in membrane fractions. Generation of the form II correlates with autophagosome association. These results suggest that all mammalian Atg8 homologues receive a common modification to associate with autophagosomal membrane as the form II.
関連製品	M135-3 Anti-GABARAP mAb PM038 Anti-GATE-16 pAb PM072 Anti-VMP1 pAb PM036 Anti-LC3 pAb M152-3 Anti-LC3 mAb M186-3 Anti-LC3 mAb PD014 Anti-LC3 pAb PM045 Anti-p62 (SQSTM1) pAb M162-3 Anti-p62 (SQSTM1) (Human) mAb PM066 Anti-p62 C-terminal pAb M133-3 Anti-Atg3 mAb PM039 Anti-Atg7 (Human) pAb PM090 Anti-Atg8 (Filamentous fungi) pAb M152-3MS Anti-LC3 mAb for My select sampler set
使用文献	使用文献募集中！本製品を使って論文を発表されましたら是非お知らせください。 Maruyama Y et al. LC3B is indispensable for selective autophagy of p62 but not basal autophagy. Biochem Biophys Res Commun. 446, 309-15 (2014)(PMID:24582747) Tanida I et al. In vitro assays of lipidation of Mammalian Atg8 homologs. Curr. Protoc. Cell Biol. 646, 11.20.1-13 (2014)(PMID:25181299) Szalai P et al. Autophagic bulk sequestration of cytosolic cargo is independent of LC3, but requires GABARAPs. Exp Cell Res. 333, 21-38 (2015)(PMID:25684710) Western Blotting Mariño G et al. Autophagy is essential for mouse sense of balance. J Clin Invest. 120, 2331-44 (2010)(PMID:20577052) Polletta L et al. SIRT5 regulation of ammonia-induced autophagy and mitophagy. Autophagy 11, 253-70 (2015)(PMID:25700560) Park S et al. O-GlcNAc modification is essential for the regulation of autophagy in Drosophila melanogaster. Cell Mol Life Sci. 72, 3173-83 (2015)(PMID:25840568) Winer H et al. Autophagy differentially regulates TNF receptor Fn14 by distinct mammalian Atg8 proteins. Nat Commun. 9, 3744 (2018)(PMID:30218067) Saladini S et al. Metformin Impairs Glutamine Metabolism and Autophagy in Tumour Cells. Cells 8, E49 (2019)(PMID:30646605) Qiao J et al. Identification of AaAtg8 as a marker of autophagy and a functional autophagy-related protein in Aedes albopictus. PeerJ. 6, e5988 (2018)(PMID:30498644) Saladini S et al. Metformin Impairs Glutamine Metabolism and Autophagy in Tumour Cells. Cells. 8, pii: E49 (2019) (PMID:30646605) Immunohistochemistry Li C et al. Spermine synthase deficiency causes lysosomal dysfunction and oxidative stress in models of Snyder-Robinson syndrome. Nat Commun. 8, 1257 (2017)(PMID:29097652)
製品カテゴリー	分野オートファジー

※在庫につきましては2024年04月18日 17時00分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
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※使用法の表記について：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
※使用法・交差性の表記について：
*: 論文で報告されております（MBLでは未確認）。詳しくはデータシートをご覧ください。
**: 導入元からの情報です（MBLでは評価中もしくは未確認）。
※保存温度の表記について： RT: 室温
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