トップ > 製品検索結果一覧 > Code No. PM036 Anti-LC3 pAb

Code No. PM036

Anti-LC3 pAb

価格(税別)

¥52,800

在庫

10以上

(2024/12/14 00:05時点)

包装

100 µL

データ
  • Western Blotting

  • Western Blotting

  • Immunoprecipitation

  • Immunocytochemistry

  • Immunohistochemistry

クローナリティー Polyclonal クローン Polyclonal
アイソタイプ
(免疫動物)
Rabbit IgG
使用法
WB
1:1,000  
IP
2 µL/300 µL of cell extract from 1x107 cells  
FCM
1:200  
IC
1:500-1:1,000  
IH
1:1,000-1:2,000  
Other(Immuno-EM)
reported.  (PMID: 28063257
免疫原(抗原) recombinant Human LC3 (MAP1LC3B:1-120 a.a.)
交差性
[Gene ID]

Human[81631/84557], Mouse[67443/66734], Rat[64862/362245], Hamster[100769810], Zebrafish*[406466](PMID: 28924380/29323117)

性状 PBS/50% glycerol, pH 7.2
保存温度 -20°C 標識 Unlabeled メーカー MBL
別称 MAP1LC3B, LC3B, ATG8F, MAP1LC3B-a, MAP1
背景 Macroautophagy mediates the bulk degradation of cytoplasmic components. These components are delivered to lysosomes via autophagosomes. The microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I. A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II. This antibody can detect both forms of LC3.
関連製品 PM036-PN Positive control for anti-LC3 antibody
M152-3 Anti-LC3 mAb
M186-3 Anti-LC3 mAb
PD014 Anti-LC3 pAb
PM072 Anti-VMP1 pAb
PM045 Anti-p62 (SQSTM1) pAb
M162-3 Anti-p62 (SQSTM1) (Human) mAb
PM066 Anti-p62 C-terminal pAb
PD017 Anti-Beclin 1 pAb
PM037 Anti-GABARAP pAb
M135-3 Anti-GABARAP mAb
PM038 Anti-GATE-16 pAb
PM039 Anti-Atg7 (Human) pAb
M133-3 Anti-Atg3 mAb
8485 Autophagy Ab Sampler Set
M152-3MS Anti-LC3 mAb for My select sampler set
M153-3 Anti-Atg5 mAb
M150-3 Anti-Atg16L mAb
8486 Autophagy Watch
M134-3 Anti-Atg4B mAb
M154-3 Anti-Atg12 (Human) mAb
M162-A48 Anti-p62 (SQSTM1) (Human) mAb-Alexa Fluor™ 488
M162-A59 Anti-p62 (SQSTM1) (Human) mAb-Alexa Fluor™ 594
M162-A64 Anti-p62 (SQSTM1) (Human) mAb-Alexa Fluor™ 647
M186-7 Anti-LC3 mAb-HRP-DirecT
M200-3 Anti-NRF2 mAb
PM040 Anti-Atg16L pAb
PM050 Anti-Atg5 pAb
備考 FAQ:よくあるお問い合わせを見る→
使用文献
使用文献募集中!本製品を使って論文を発表されましたら是非お知らせください。

Western Blotting

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Immunoprecipitation

  1. Botbol Y et al. Common γ-chain cytokine signaling is required for macroautophagy induction during CD4+ T-cell activation. Autophagy 11, 1864-77 (2015)(PMID:26391567)
  2. Kido K et al. AirID, a novel proximity biotinylation enzyme, for analysis of protein-protein interactions. Elife. 9, e54983 (2020)(PMID:32391793)

Immunocytochemistry

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  3. Taguwa S et al. Dysfunction of autophagy participates in vacuole formation and cell death in cells replicating hepatitis C virus. J Virol. 85, 13185-94 (2011)(PMID:21994453)
  4. Rasmussen SB et al. Activation of autophagy by α-herpesviruses in myeloid cells is mediated by cytoplasmic viral DNA through a mechanism dependent on stimulator of IFN genes. J Immunol. 187, 5268-76 (2011)(PMID:21998456)
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  8. Botbol Y et al. Common γ-chain cytokine signaling is required for macroautophagy induction during CD4+ T-cell activation. Autophagy 11, 1864-77 (2015)(PMID:26391567)
  9. Botbol Y, Macian F. Assays for Monitoring Macroautophagy Activity in T cells. Methods Mol Biol. 1343, 143-53 (2015)(PMID:26420715)
  10. Cherepkova MY et al. Leukemia inhibitory factor (LIF) withdrawal activates mTOR signaling pathway in mouse embryonic stem cells through the MEK/ERK/TSC2 pathway. Cell Death Dis. 7, e2050 (2016)(PMID:26775702)
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  13. Girault V et al. Prenatal alcohol exposure impairs autophagy in neonatal brain cortical microvessels. Cell Death Dis. 8, e2610 (2017)(PMID:28182007)
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  17. Lu SL et al. Endothelial cells are intrinsically defective in xenophagy of Streptococcus pyogenes. PLoS Pathog. 13, e1006444 (2017)(PMID:28683091)
  18. Ono A et al. Feedback activation of AMPK-mediated autophagy acceleration is a key resistance mechanism against SCD1 inhibitor-induced cell growth inhibition. PLoS One. 12, e0181243 (2017)(PMID:28704514)
  19. Chen YD et al. Exophagy of annexin A2 via RAB11, RAB8A and RAB27A in IFN-γ-stimulated lung epithelial cells. Sci Rep. 7, 5676 (2017)(PMID:28720835)
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  21. Kjos I et al. Rab7b modulates autophagic flux by interacting with Atg4B. EMBO Rep. 18, 1727-1739 (2017)(PMID:28835545)
  22. Meng XH et al. Intracellular Insulin and Impaired Autophagy in a Zebrafish model and a Cell Model of Type 2 diabetes. Int J Biol Sci. 13, 985-995 (2017)(PMID:28924380)
  23. Wang Y et al. Mitochondrial Fission Promotes the Continued Clearance of Apoptotic Cells by Macrophages. Cell 171, 331-345.e22 (2017)(PMID:28942921)
  24. Lee PP et al. Wiskott-Aldrich syndrome protein regulates autophagy and inflammasome activity in innate immune cells. Nat Commun. 8, 1576 (2017)(PMID:29146903)
  25. Yanagisawa H et al. L-leucine and SPNS1 coordinately ameliorate dysfunction of autophagy in mouse and human Niemann-Pick type C disease. Sci Rep. 7, 15944 (2017)(PMID:29162837)
  26. Hsu CL et al. MAP4K3 mediates amino acid-dependent regulation of autophagy via phosphorylation of TFEB. Nat Commun. 9, 942 (2018)(PMID:29507340)
  27. Sun Y et al. 3′-epi-12β-hydroxyfroside, a new cardenolide, induces cytoprotective autophagy via blocking the Hsp90/Akt/mTOR axis in lung cancer cells. Theranostics 8, 2044-2060 (2018)(PMID:29556372)
  28. El-Darawish Y et al. Frontline Science: IL-18 primes murine NK cells for proliferation by promoting protein synthesis, survival, and autophagy. J Leukoc Biol. 104, 253-264 (2018)(PMID:29603367)
  29. Raulien N et al. Fatty Acid Oxidation Compensates for Lipopolysaccharide-Induced Warburg Effect in Glucose-Deprived Monocytes. Front Immunol. 8, 609 (2017)(PMID:28611773)
  30. Härtlova A et al. LRRK2 is a negative regulator of Mycobacterium tuberculosis phagosome maturation in macrophages. EMBO J. 37, e98694 (2018)(PMID:29789389)
  31. Phadwal K et al. Spermine increases acetylation of tubulins and facilitates autophagic degradation of prion aggregates. Sci Rep. 8, 10004 (2018)(PMID:29968775)
  32. Skah S et al. cAMP-mediated autophagy inhibits DNA damage-induced death of leukemia cells independent of p53. Oncotarget 9, 30434–30449 (2018)(PMID:30100998)
  33. Hagio-Izaki K et al. Lipopolysaccharide induces bacterial autophagy in epithelial keratinocytes of the gingival sulcus. BMC Cell Biol. 19, 18 (2018)(PMID:30165815)
  34. Jiao YN et al. Marsdenia tenacissima extract induces apoptosis and suppresses autophagy through ERK activation in lung cancer cells. Cancer Cell Int. 18, 149 (2018)(PMID:30275772)
  35. Kim JK et al. GABAergic signaling linked to autophagy enhances host protection against intracellular bacterial infections. Nat Commun. 9, 4184 (2018)(PMID:30305619)
  36. Mori H et al. Induction of selective autophagy in cells replicating hepatitis C virus genome. J Gen Virol. 99, 1643-1657 (2018)(PMID:30311874)
  37. Janssen AFJ et al. Probing aggrephagy using chemically-induced protein aggregates. Nat Commun. 9, 4245 (2018)(PMID:30315152)
  38. Schulthess J et al. The Short Chain Fatty Acid Butyrate Imprints an Antimicrobial Program in Macrophages. Immunity. 50, 432-445.e7 (2019)(PMID:30683619)
  39. Ma B et al. The SIAH2-NRF1 axis spatially regulates tumor microenvironment remodeling for tumor progression. Nat Commun. 10, 1034 (2019)(PMID:30833558)
  40. Ding X et al. RAB2 regulates the formation of autophagosome and autolysosome in mammalian cells. Autophagy. 15, 1774-1786 (2019)(PMID:30957628)
  41. Nakashima A et al. Endoplasmic reticulum stress disrupts lysosomal homeostasis and induces blockade of autophagic flux in human trophoblasts. Sci Rep. 9, 11466 (2019)(PMID:31391477)
  42. Nakamura T et al. Soluble Lytic Transglycosylase SLT of Francisella Novicida Is Involved in Intracellular Growth and Immune Suppression. PLoS One. 14, e0226778 (2019) (PMID:31877174)
  43. Omi J et al. The Inducible Amphisome Isolates Viral Hemagglutinin and Defends Against Influenza A Virus Infection. Nat Commun. 11, 162 (2020)(PMID:31919357)
  44. Dong S et al. Monitoring Spatiotemporal Changes in Chaperone-Mediated Autophagy in Vivo. Nat Commun. 11, 645 (2020)(PMID:32005807)
  45. Siedlecka-Kroplewska K et al. The wine polyphenol resveratrol modulates autophagy and induces apoptosis in MOLT-4 and HL-60 human leukemia cells. J Physiol Pharmacol. 70 (2020)(PMID:32084644)
  46. Nozaki M et al. SQSTM1 L341V variant that is linked to sporadic ALS exhibits impaired association with MAP1LC3 in cultured cells. eNeurologicalSci. 22, 100301 (2020)(PMID:33319079)

Immunohistochemistry

  1. Hasui K et al. Enhanced Autophagy and Reduced Expression of Cathepsin D Are Related to Autophagic Cell Death in Epstein-Barr Virus-Associated Nasal Natural Killer/T-Cell Lymphomas: An Immunohistochemical Analysis of Beclin-1, LC3, Mitochondria (AE-1), and Cathepsin D in Nasopharyngeal Lymphomas. Acta Histochem Cytochem. 44, 119-31 (2011)(PMID:21753858)
  2. Girault V et al. Prenatal alcohol exposure impairs autophagy in neonatal brain cortical microvessels. Cell Death Dis. 8, e2610 (2017)(PMID:28182007)
  3. Qi M et al. ERK inhibition represses gefitinib resistance in non-small cell lung cancer cells. Oncotarget. 9, 12020-12034 (2018)(PMID:29552290)
  4. Ko PY et al. Schwann-Cell Autophagy, Functional Recovery, and Scar Reduction After Peripheral Nerve Repair. J Mol Neurosci. 64, 601-610 (2018)(PMID:29644600)
  5. Alvarez-Garcia O et al. FOXO are required for intervertebral disk homeostasis during aging and their deficiency promotes disk degeneration. Aging Cell. 17, e12800 (2018)(PMID:29963746)
  6. Horibe A et al. Ethanol-Induced Autophagy in Sertoli Cells Is Specifically Marked at Androgen-Dependent Stages of the Spermatogenic Cycle: Potential Mechanisms and Implications. Int J Mol Sci. 20, pii: E184 (2019)(PMID:30621351)
  7. Lu SZ et al. Suppression of astrocytic autophagy by αB-crystallin contributes to α-synuclein inclusion formation. Transl Neurodegener. 8, 3 (2019)(PMID:30675347)
  8. Hsu YH et al. Traffic-related particulate matter exposure induces nephrotoxicity in vitro and in vivo. Free Radic Biol Med. 135, 235-244 (2019)(PMID:30878646)
  9. Eid N et al. Ethanol-Induced Mitochondrial Damage in Sertoli Cells is Associated with Parkin Overexpression and Activation of Mitophagy. Cells. 8, pii: E283 (2019)(PMID:30934625)
  10. Boukhalfa A et al. PI3KC2α-dependent and VPS34-independent Generation of PI3P Controls Primary Cilium-Mediated Autophagy in Response to Shear Stress. Nat Commun. 11, 294 (2020) (PMID:31941925)
  11. Ogawa-Akiyama A et al. Podocyte Autophagy Is Associated With Foot Process Effacement and Proteinuria in Patients With Minimal Change Nephrotic Syndrome. PLoS One. 15, e0228337 (2020)(PMID:31978139)
  12. Kurashige T et al. Hormonal Regulation of Autophagy in Thyroid PCCL3 Cells and the Thyroids of Male Mice. J Endocr Soc. 4, bvaa054 (2020)(PMID:32671315)

Immunofluorescence

  1. Nakajima S et al. Fat-specific protein 27α inhibits autophagy-dependent lipid droplet breakdown in white adipocytes. J Diabetes Investig. in press.(PMID:30927519)
  2. Scotto Rosato A et al. TRPML1 Links Lysosomal Calcium to Autophagosome Biogenesis Through the Activation of the CaMKKβ/VPS34 Pathway. Nat Commun. 10, 5630 (2019)(PMID:31822666)
  3. Luciani A et al. Impaired mitophagy links mitochondrial disease to epithelial stress in methylmalonyl-CoA mutase deficiency. Nat Commun. 11, 970 (2020)(PMID:32080200)
  4. Lee J et al. 4-Hydroxybenzaldehyde Restricts the Intracellular Growth of Toxoplasma gondii by Inducing SIRT1-Mediated Autophagy in Macrophages. Korean J Parasitol. 58, 7-14 (2020)(PMID:32145722)
  5. Kurashige T et al. Hormonal Regulation of Autophagy in Thyroid PCCL3 Cells and the Thyroids of Male Mice. J Endocr Soc. 4, bvaa054 (2020)(PMID:32671315)
  6. Chen C et al. Blockade of HCN2 Channels Provides Neuroprotection Against Ischemic Injury via Accelerating Autophagic Degradation in Hippocampal Neurons. Neurosci Bull. 36, 875-894 (2020)(PMID:32519067)
  7. Nettesheim A et al. Autophagy in the Aging and Experimental Ocular Hypertensive Mouse Model. Invest Ophthalmol Vis Sci. 61, 31 (2020)(PMID:32797200)
  8. Bosc C et al. Autophagy regulates fatty acid availability for oxidative phosphorylation through mitochondria-endoplasmic reticulum contact sites. Nat Commun. 11, 4056 (2020)(PMID:32792483)

Other(Immuno-EM)

  1. Fujita N et al. Genetic screen in Drosophila muscle identifies autophagy-mediated T-tubule remodeling and a Rab2 role in autophagy. Elife. 6, e23367 (2017)(PMID:28063257)
  2. Eid N et al. Ethanol-Induced Mitochondrial Damage in Sertoli Cells is Associated with Parkin Overexpression and Activation of Mitophagy. Cells. 8, pii: E283 (2019)(PMID:30934625)
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※使用法の表記について:
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
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