トップ > 製品検索結果一覧 > Code No. M152-3MS Anti-LC3 mAb for My select sampler set

Code No. M152-3MS

Anti-LC3 mAb for My select sampler set

価格(税別)

¥9,600

在庫

10以上

(2019/11/14 17:00時点)

包装

40 µg/20 µL

データ
  • Western Blotting

  • Immunoprecipitation

クローナリティー Monoclonal クローン 4E12
アイソタイプ
(免疫動物)
Mouse IgG1 κ
使用法
WB
5 µg/mL  
IP
5 µg/300 µL of cell extract from 1x107 cells  
FCM
40 µg/mL (final concentration)  
IC
40 µg/mL  
IH*
reported.  (PMID: 21957238 / 19955365 / 19915056
Other(Immuno-EM)
20 µg/mL  
免疫原(抗原) Recombinant Human LC3 (MAP1LC3B: 1-120 a.a.)
交差性
[Gene ID]

Human[81631/ 84557], Mouse[67443/ 66734], Rat[64862/ 362245], Hamster[100760576/ 100769810]

性状 2 mg/mL in PBS/50% glycerol, pH 7.2
保存温度 -20°C 標識 Unlabeled メーカー MBL
別称 microtubule-associated protein 1 light chain 3 beta, microtubule-associated protein 1 light chain 3 alpha, LC3B, ATG8F, MAP1LC3B-a, MAP1A/1BLC3, MAP1LC3B, LC3A, ATG8E, MAP1ALC3, MAP1BLC3, MAP1LC3A
背景 Macroautophagy mediates the bulk degradation of cytoplasmic components. These components are delivered to lysosomes via autophagosomes. The rat microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I. A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II. This antibody can detect both forms of LC3.
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備考 FAQ:よくあるお問い合わせを見る→
本製品はMy select sampler set対象品です。詳細はこちらをご覧ください。
使用文献
使用文献募集中!本製品を使って論文を発表されましたら是非お知らせください。

Western Blotting

  1. Ho H et al. WIPI1 coordinates melanogenic gene transcription and melanosome formation via TORC1 inhibition. J Biol Chem. 286, 12509-23 (2011)(PMID:21317285)
  2. Saiki S et al. Caffeine induces apoptosis by enhancement of autophagy via PI3K/Akt/mTOR/p70S6K inhibition. Autophagy 7, 176-187 (2011)(PMID:21081844)
  3. Kasai M et al. Autophagic compartments gain access to the MHC class II compartments in thymic epithelium. J Immunol. 183, 7278-85 (2009)(PMID:19915056)
  4. Wang Y et al. Loss of macroautophagy promotes or prevents fibroblast apoptosis depending on the death stimulus. J Biol Chem. 283, 4766-77 (2008)(PMID:18073215)
  5. Murthy A et al. A Crohn's disease variant in Atg16l1 enhances its degradation by caspase 3. Nature 506, 456-62 (2014)(PMID:24553140)

Immunoprecipitation

  1. Mookerjee S et al. Posttranslational modification of ataxin-7 at lysine 257 prevents autophagy-mediated turnover of an N-terminal caspase-7 cleavage fragment. J Neurosci. 29, 15134-44 (2009)(PMID:19955365)

Immunocytochemistry

  1. Kasai M et al. Autophagic compartments gain access to the MHC class II compartments in thymic epithelium. J Immunol. 183, 7278-85 (2009)(PMID:19915056)
  2. Nishitsuji K et al. The E693Delta mutation in amyloid precursor protein increases intracellular accumulation of amyloid beta oligomers and causes endoplasmic reticulum stress-induced apoptosis in cultured cells. Am J Pathol. 174, 957-69 (2009)(PMID:19164507)
  3. Wang Y et al. Loss of macroautophagy promotes or prevents fibroblast apoptosis depending on the death stimulus. J Biol Chem. 283, 4766-77 (2008)(PMID:18073215)
  4. Chalazonitis A et al. Homeodomain interacting protein kinase 2 regulates postnatal development of enteric dopaminergic neurons and glia via BMP signaling. J Neurosci. 31, 13746-57 (2011)(PMID:21957238)
  5. Mori H et al. Induction of selective autophagy in cells replicating hepatitis C virus genome. J Gen Virol. 99, 1643-1657 (2018)(PMID:30311874)

Immunohistochemistry

  1. Kasai M et al. Autophagic compartments gain access to the MHC class II compartments in thymic epithelium. J Immunol. 183, 7278-85 (2009)(PMID:19915056)
  2. Mookerjee S et al. Posttranslational modification of ataxin-7 at lysine 257 prevents autophagy-mediated turnover of an N-terminal caspase-7 cleavage fragment. J Neurosci. 29, 15134-44 (2009)(PMID:19955365)

Other(Immuno-EM)

  1. Murthy A et al. A Crohn's disease variant in Atg16l1 enhances its degradation by caspase 3. Nature 506, 456-62 (2014)(PMID:24553140)
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※在庫につきましては2019年11月14日 17時00分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について:
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
※使用法・交差性の表記について:
*: 論文で報告されております(MBLでは未確認)。詳しくはデータシートをご覧ください。
**: 導入元からの情報です(MBLでは評価中もしくは未確認)。
※保存温度の表記について: RT: 室温
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