| |
Ultracentrifugation method |
Polymer-based precipitatin method |
Immunoaffinity method |
| Method |
- Exosome isolation method utilizing difference of density
|
- Exosome precipitation method using polymer
|
- Immunoaffinity method using magnetic beads-conjugated antibodies against exsomal markers
|
| Advantage |
- Conventional, standard method
- Sutable for large scale
- Recover Exosome from variety of samples
- Low cost
|
- Easy operation
- Recovery of Exosome in a short period of time
- No specific device, instrument is required
|
- Easy operation
- Recovery of Exosome in a short period of time
- Few contaminants with high purity
- Enable to isolate exosomes that express specific marker
|
| Disadvantage |
- Takes time with complicated oparation (needs half a day - 2 days)
- Many contaminants with poor purification
- Difficlut to use multipe samples simultaneously
- Required for optimized experimental condition to avoid vesicle loss
- Ultracentrifuge is required
|
- Many contaminants with poor purification
- Need to select the optimal reagents depending on the sample types (e.g. serum, plasma, culuture sup)
|
- Exosome recovery depends on antibody specificity
|
| MBL products |
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