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“Biotinylated Antibodyアイコン"

Biotinylated Antibody




  • Biotin-labeled antibodies are against CD9, CD63, CD81, and EpCAM.
  • Use with the Exocap™ Streptavidin Kit for isolation of exosomes.
  • Also suitable for sandwich ELISA and CLEIA.

Examples of use

Western Blotting analysis of exosomes isolated from HT29 cell culture spernatant and serum and plasma from healthy individuals

Exosomes were isolated using ExoCap™ Streptavidin Kit and biotinylated antibodies (Anti-CD9 mAb-Biotin (Code No. MEX001-6) or Anti-CD63(LAMP-3) mAb-Biotin (Code No. MEX002-6)).
In each sample, stronger signal was observed with ExoCap™ compared to the ultracentrifugation method and Competitive Product A.

Western Blotting

1. Input of HT29 sup.
2. Ultracentrifigation
3. Competitor A + Anti-CD9 mAb biotin (Code No. MEX001-6)
4. Exocap™ + Anti-CD9 mAb biotin (Code No. MEX001-6)
5. Competitor A + Anti-CD63 mAb biotin (Code No. MEX002-6)
6. Exocap™ + Anti-CD63 mAb biotin (Code No. MEX002-6)



[Detection of contaminants] Presence of contaminants in the above samples was checked by SDS-PAGE and CBB staining.

CBB staining

Non-specific protein binding was lower with the ExoCap™ Streptavidin Kit compared to the competitive product.

1. Ultracentrifigation
2. Competitor A + Anti-CD9 mAb biotin
3. ExoCap™ + Anti-CD9 mAb biotin
4. Competitor A + Anti-EpCAM mAb biotin
5. ExoCap™ + Anti-EpCAM mAb biotin


Western Blotting analysis of exosomes isolated from serum from healthy individuals.


Flow cytometric analysis of exosomes isolated from the plasma of healthy individuals

Flow cytometric analysis
Note: The magnetic beads in Competitive Product A emit autofluorescence, while little autofluorescence is detected with the magnetic beads in ExoCap™

Flow cytometric analysis of exosomes isolated from HT29 cell culture supernatant

S/N ratio
Exosomes were isolated from HT29 cell culture supernatant using the ExoCap™ Streptavidin Kit or Streptavidin beads from the competitor with biotin-labeled CD9 antibody, and FCM analysis was performed. The S/N ratio was better with the ExoCap™ Streptavidin Kit compared to the beads from the competitor.

Detection of HT29-derived exosomes by CLEIA

CLEIA

qRT-PCR detection of miR-21 in exosomes isolated from HT29 cell culture supernatant and serum and plasma from healthy individuals

qRT-PCR
Exosomes were isolated using the ExoCap™ Streptavidin Kit (Code No. MEX-SA) and Anti-CD9 mAb-Biotin (Code No. MEX001-6). Nucleic acids were purified using ExoCap™ Nucleic Acid Elution Buffer (Code No. MEX-E) and analyzed by qRT-PCR to detect miR-21.


“ExoCap” is a trademark of JSR Corporation.


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