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“ExoCap Streptavidin CD9/CD63/CD81 Setアイコン"

ExoCap™ Streptavidin CD9/CD63/CD81 Set



Reagents for exosome isolation from human-derived samples

  • High-purity exosomes can be isolated by a simple procedure
  • Isolated exosomes can be analyzed by WB, qRT-PCR, CLEIA, ELISA, and FCM

Components

Components
Biotinylated antibodies to CD9, CD63, and CD81 are highly specific and perform excellently in exosome isolation by immunoprecipitation.
The ExoCap™ Streptavidin Kit consists of streptavidin-immobilized magnetic beads (Magnosphere™) and buffer solutions for capturing, washing, and extracting exosomes. The Magnosphere™ is coated with a JSR Life Sciences Corporation proprietary hydrophilic polymer to decrease non-specific binding. It has been used in in vitro diagnostic use. You can isolate exosomes in high-purity by using it together with our original biotinylated specific antibodies to CD9, CD63, and CD81.


Outline of ExoCap™ Streptavidin CD9/CD63/CD81 Set protocol

1. Preparation of Capture Beads Mix
Preparation of Capture Beads Mix
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2. Isolation of exosomes
Isolation of exosomes
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Examples of use

1. Western blot analysis of isolated exosomes
mex-sa123-WB
After allowing the biotinylated CD9, CD63, and CD81 antibodies to bind separately to Streptavidin Magnetic Beads, an equal volume of each antibody-bound beads was mixed together (CD9/CD63/CD81 Mix). Using the mixed beads, exosomes were isolated from each sample and subjected to Western blot analysis.


2. qRT-PCR analysis of miR-21 in exosomes
mex-sa123-qRT-PCR
After allowing the biotinylated CD9, CD63, and CD81 antibodies to bind separately to Streptavidin Magnetic Beads, an equal volume of each antibody-bound beads was mixed together (CD9/CD63/CD81 Mix). Using the mixed beads, exosomes were isolated from each sample. Nucleic acid was purified with ExoCap™ Nucleic Acid Elution Buffer (Code No. MEX-E) and subjected to qRT-PCR to detect miR-21.



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"ExoCap" is a registered trademark of JSR Corporation.