ExoCap™ Streptavidin Kit is designed for customized isolation and analysis of exosomes or microvesicles, called Extracellura Vesicles (EVs), using the researcher's biotinylated molecules such as antibodies against exosome surface marker proteins. ExoCap™ uses functionalized Magnospere™, magnetic micro-particles coated with JSR Life Sciences proprietary hydrophilic polymer to decrease non-specific binding.
Biotinylated antibodies for CD9, CD63, CD81, and EpCAM are available. Please check our biotinylated antibodies!
ExoCap™ Streptavidin Kit using with biotinylated antibodies (Anti-CD9 mAb-Biotin (Code No. MEX001-6) and Anti-CD63 (LAMP-3) mAb-Biotin (Code No. MEX002-6)).
In each sample, stronger signal was observed with ExoCap™ compared to the ultracentrifugation method and Competitive Product A.
3. Competitor A + Anti-CD9 mAb biotin (Code No. MEX001-6)
4. ExoCap™ + Anti-CD9 mAb biotin (Code No. MEX001-6)
5. Competitor A + Anti-CD63 mAb biotin (Code No. MEX002-6)
6. ExoCap™ + Anti-CD63 mAb biotin (Code No. MEX002-6)
Non-specific protein binding was lower with the ExoCap™ Streptavidin Kit compared to the competitive product.
2. Competitor A + Anti-CD9 mAb biotin
3. ExoCap™ + Anti-CD9 mAb biotin
4. Competitor A + Anti-EpCAM mAb biotin
5. ExoCap™ + Anti-EpCAM mAb biotin
Note: The magnetic beads in Competitive Product A emit autofluorescence, while little autofluorescence is detected with the magnetic beads in ExoCap™
Exosomes were isolated from HT29 cell culture supernatant using the ExoCap™ Streptavidin Kit or Streptavidin beads from the competitor with biotinylated CD9 antibody, and FCM analysis was performed. The S/N ratio was better with the ExoCap™ Streptavidin Kit compared to the beads from the competitor.
Exosomes were isolated using the ExoCap™ Streptavidin Kit (Code No. MEX-SA) and biotinylated CD9 antibody (Code No. MEX001-6) Nucleic acids were purified using ExoCap™ Nucleic Acid Elution Buffer (Code No. MEX-E) and analyzed by qRT-PCR to detect miR-21.
“ExoCap” is a trademark of JSR Corporation.