This site is for customers in Japan. Customers in other regions, please go to Global page.
HOME > Product category > Exosomes > Featured products > ExoCap Streptavidin Kit
“ExoCap Streptavidin Kitアイコン"

ExoCap™ Streptavidin Kit




  • Flexible use of biotinylated antibodies to target exosoms
  • Lower non-specific binding by new "streptavidin magnetic beads" and buffer
  • Easy to use
  • Suitable for Flow cytometry, Sandwich ELISA and CLEIA

ExoCap™ Streptavidin Kit is designed for customized isolation and analysis of exosomes or microvesicles, called Extracellura Vesicles (EVs), using the researcher's biotinylated molecules such as antibodies against exosome surface marker proteins. ExoCap™ uses functionalized Magnospere™, magnetic micro-particles coated with JSR Life Sciences proprietary hydrophilic polymer to decrease non-specific binding.
Biotinylated antibodies for CD9, CD63, CD81, and EpCAM are available. Please check our biotinylated antibodies!


Procedure Summary

Procedure summary

Example of uses

Western Blotting analysis of exosomes isolated form HT29 cell culture supernatant and serum and plasma from healthy individuals

ExoCap™ Streptavidin Kit using with biotinylated antibodies (Anti-CD9 mAb-Biotin (Code No. MEX001-6) and Anti-CD63 (LAMP-3) mAb-Biotin (Code No. MEX002-6)).
In each sample, stronger signal was observed with ExoCap™ compared to the ultracentrifugation method and Competitive Product A.

Western blotting

1. Input
2. Ultracentrifigation
3. Competitor A + Anti-CD9 mAb biotin (Code No. MEX001-6)
4. ExoCap™ + Anti-CD9 mAb biotin (Code No. MEX001-6)
5. Competitor A + Anti-CD63 mAb biotin (Code No. MEX002-6)
6. ExoCap™ + Anti-CD63 mAb biotin (Code No. MEX002-6)

[Detection of contaminants] Presence of contaminants in the above samples was checked by SDS-PAGE and CBB staining.

CBB staining

Non-specific protein binding was lower with the ExoCap™ Streptavidin Kit compared to the competitive product.

1. Ultracentrifigation
2. Competitor A + Anti-CD9 mAb biotin
3. ExoCap™ + Anti-CD9 mAb biotin
4. Competitor A + Anti-EpCAM mAb biotin
5. ExoCap™ + Anti-EpCAM mAb biotin


Flow cytometric analysis of exosomes isolated from the plasma of healthy individuals

Flow cytometric analysis
Note: The magnetic beads in Competitive Product A emit autofluorescence, while little autofluorescence is detected with the magnetic beads in ExoCap™

Flow cytometric analysis of exosomes isolated from HT29 cell culture supernatant

S/N ratio
Exosomes were isolated from HT29 cell culture supernatant using the ExoCap™ Streptavidin Kit or Streptavidin beads from the competitor with biotinylated CD9 antibody, and FCM analysis was performed. The S/N ratio was better with the ExoCap™ Streptavidin Kit compared to the beads from the competitor.

Detection of HT29-derived exosomes by CLEIA

CLIEA

CLEIA analysis of HT29 cell culture supernatant and serum

qRT-PCR
Exosomes were isolated using the ExoCap™ Streptavidin Kit (Code No. MEX-SA) and biotinylated CD9 antibody (Code No. MEX001-6) Nucleic acids were purified using ExoCap™ Nucleic Acid Elution Buffer (Code No. MEX-E) and analyzed by qRT-PCR to detect miR-21.


Related products


“ExoCap” is a trademark of JSR Corporation.