多能性幹細胞オルガノイドの応用 論文リスト
多能性幹細胞由来のオルガノイド研究は、各器官の分化メカニズムを解明し、疾患の原因究明や薬剤試験、再生医療への応用につながっています。当ページでは各オルガノイドを利用した試験系の構築に関する文献を中心に掲載しました。
＜脳オルガノイド＞
- ヒトの大脳皮質の発達と小頭症のモデリング
Lancaster MA et al. Cerebral organoids model human brain development and microcephaly. Nature. 501, 373-9 (2013). [PMID: 23995685] - ヒト胎児新皮質発達のモデリング
Camp JG et al. Human cerebral organoids recapitulate gene expression programs of fetal neocortex development. Proc Natl Acad Sci U S A. 112, 15672-7 (2015). [PMID: 26644564] - 自閉症スペクトラム障害のモデリング
Mariani J et al. FOXG1-Dependent Dysregulation of GABA/Glutamate Neuron Differentiation in Autism Spectrum Disorders. Cell. 162, 375-390 (2015). [PMID: 26186191] - ジカウイルス感染のモデリング
Cugola FR et al. The Brazilian Zika virus strain causes birth defects in experimental models. Nature. 534, 267-71 (2016). [PMID: 27279226] - アプリケーション応用の可能性：人間の脳の発達と疾患モデリング、および薬物検査
Qian X et al. Brain-Region-Specific Organoids Using Mini-bioreactors for Modeling ZIKV Exposure. Cell. 165, 1238-1254 (2016). [PMID: 27118425]
Garcez PP et al. Zika virus impairs growth in human neurospheres and brain organoids. Science. 352, 816-8 (2016). [PMID: 27064148] - ゼッケル症候群のモデリング
Gabriel E et al. CPAP promotes timely cilium disassembly to maintain neural progenitor pool. EMBO J. 35, 803-19 (2016). [PMID: 26929011] - 霊長類の種の違いのモデリング
Otani T et al. 2D and 3D Stem Cell Models of Primate Cortical Development Identify Species-Specific Differences in Progenitor Behavior Contributing to Brain Size. Cell Stem Cell. 18, 467-80 (2016). [PMID: 27049876] - 大脳皮質の発達と病気のモデリング
Pasca AM et al. Functional cortical neurons and astrocytes from human pluripotent stem cells in 3D culture. Nat Methods. 12, 671-8 (2015). [PMID: 26005811]
＜網膜オルガノイド＞
- 緑内障のモデリング
Tucker BA et al. Duplication of TBK1 Stimulates Autophagy in iPSC-derived Retinal Cells from a Patient with Normal Tension Glaucoma. J Stem Cell Res Ther. 3, 161 (2014). [PMID: 24883232]
＜腸オルガノイド＞
- 腸の発達応用の可能性：腸疾患のモデリング
Spence JR et al. Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro. Nature. 470, 105-9 (2011). [PMID: 21151107] - 小腸の切除及び移植における応答
Watson CL et al. An in vivo model of human small intestine using pluripotent stem cells. Nat Med. 20, 1310-4 (2014). [PMID: 25326803]
＜肝オルガノイド＞
- 再生医療のための臓器芽移植
Takebe T et al. Vascularized and functional human liver from an iPSC-derived organ bud transplant. Nature. 499, 481-4 (2013). [PMID: 23823721] - アラジール症候群、多嚢胞肝疾患、嚢胞性線維症に関連する胆管症のモデリングと薬剤の検証
Sampaziotis F et al. Cholangiocytes derived from human induced pluripotent stem cells for disease modeling and drug validation. Nat Biotechnol. 33, 845-852 (2015). [PMID: 26167629] - 胆汁の発達と嚢胞性線維症の研究
Ogawa M et al. Directed differentiation of cholangiocytes from human pluripotent stem cells. Nat Biotechnol. 33, 853-61 (2015). [PMID: 26167630]
＜肺オルガノイド＞
- 肺の発達研究応用の可能性：肺疾患のモデリング
Dye BR et al. In vitro generation of human pluripotent stem cell derived lung organoids. Elife.4:e05098 (2015). [PMID: 25803487]
＜腎オルガノイド＞
- 応用の可能性：腎臓病のモデリング、腎毒性のスクリーニング、細胞療法
Takasato M et al. Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis. Nature. 526, 564-8 (2015). [PMID: 26444236]
＜胃オルガノイド＞
- 胃の発達とピロリ菌感染のモデリング
McCracken KW et al. Modelling human development and disease in pluripotent stem-cell-derived gastric organoids. Nature. 516, 400-4 (2014). [PMID: 25363776]
