Anti-PTBP1 (Human) pAb

価格（税別）

¥66,000

在庫

10以上

（2024/04/19 00:05時点）

包装

200 µL (1 mg/mL)

使用文献

データシート

SDS

データ	Western Blotting Immunoprecipitation RNP Immunoprecipitation
クローナリティー	Polyclonal	クローン	Polyclonal
アイソタイプ（免疫動物）	Rabbit Ig (aff.)
使用法	WB 1:1,000 IP 5 µg/250 µL of cell extract from 2.5 x 10⁶ cells IC* reported. （PMID: 24865424 / 26990993） RIP 15 µg/500 µL of cell extract from 6 x 10⁶ cells Other(eCLIP) reported. （PMID: 27018577）
免疫原(抗原)	KLH-conjugated synthetic peptide KFKGDSRSAGVPSR (46-59 a. a.)
交差性 [Gene ID]	Human[5725], Mouse(-), Rat(-), Hamster(-)
性状	1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度	-20°C	標識	Unlabeled	メーカー	MBL
別称	polypyrimidine tract binding protein 1, HNRNP-I, HNRNPI, HNRPI
背景	The polypyrimidine tract binding protein 1 (PTBP1), a 57 kDa RNA binding protein also known as hnRNP1, has been implicated in multiple aspect of mRNA metabolism, including regulation of alternative splicing, internal ribosome entry site-driven translation, mRNA localization, and polyadenylation. Four of tandem RNA recognition motif domains give PTBP1 a strong binding activity to RNA. Posttranscriptional regulation of gene expression by PTBP1 has been reported on many genes, including CD154, insulin, VEGF and Human inducible nitric oxide synthase.
関連製品	RN1001 RIP-Assay Kit RN1005 RIP-Assay Kit for microRNA RN052PW Anti-HNRNPC pAb RN060PW Anti-HNRNPD (AUF1) pAb RN019P Anti-HNRNPK pAb RN024P Anti-PCBP1 pAb RN025P Anti-PCBP2 pAb RN011P Anti-PTBP1 (Human) pAb RN114PW Anti-HNRNPA1 pAb
使用文献	使用文献募集中！本製品を使って論文を発表されましたら是非お知らせください。 Western Blotting Ferrarese R et al. Lineage-specific splicing of a brain-enriched alternative exon promotes glioblastoma progression. J Clin Invest. 124, 2861-74 (2014)(PMID:24865424) Liu W et al. Hepatitis B virus core protein inhibits Fas-mediated apoptosis of hepatoma cells via regulation of mFas/FasL and sFas expression. FASEB J. 29, 1113-23 (2015)(PMID:25466893) Cui J, Placzek WJ. PTBP1 modulation of MCL1 expression regulates cellular apoptosis induced by antitubulin chemotherapeutics. Cell Death Differ. 23, 1681-90 (2016)(PMID:27367564) Nussbacher JK, Yeo GW. Systematic Discovery of RNA Binding Proteins that Regulate MicroRNA Levels. Mol Cell. 69, 1005-1016.e7 (2018)(PMID:29547715) Cui J, Placzek WJ. PTBP1 enhances miR-101-guided AGO2 targeting to MCL1 and promotes miR-101-induced apoptosis. Cell Death Dis. 9, 552 (2018)(PMID:29748555) Immunoprecipitation Ferrarese R et al. Lineage-specific splicing of a brain-enriched alternative exon promotes glioblastoma progression. J Clin Invest. 124, 2861-74 (2014)(PMID:24865424) Immunocytochemistry Ferrarese R et al. Lineage-specific splicing of a brain-enriched alternative exon promotes glioblastoma progression. J Clin Invest. 124, 2861-74 (2014)(PMID:24865424) Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993) RNP Immunoprecipitation Ferrarese R et al. Lineage-specific splicing of a brain-enriched alternative exon promotes glioblastoma progression. J Clin Invest. 124, 2861-74 (2014)(PMID:24865424) Cui J, Placzek WJ. PTBP1 modulation of MCL1 expression regulates cellular apoptosis induced by antitubulin chemotherapeutics. Cell Death Differ. 23, 1681-90 (2016)(PMID:27367564) Wang ZN et al. High expression of PTBP1 promote invasion of colorectal cancer by alternative splicing of cortactin. Oncotarget 8, 36185-36202 (2017)(PMID:28404950) Other(eCLIP) Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993) Van Nostrand EL et al. Robust transcriptome-wide discovery of RNA-binding protein binding sites with enhanced CLIP (eCLIP). Nat Methods. 13, 508-14 (2016)(PMID:27018577)
製品カテゴリー	分野 RNA-RNPネットワークブランド RiboCluster Profiler™

※在庫につきましては2024年04月19日 00時05分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
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※使用法の表記について：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
※使用法・交差性の表記について：
*: 論文で報告されております（MBLでは未確認）。詳しくはデータシートをご覧ください。
**: 導入元からの情報です（MBLでは評価中もしくは未確認）。
※保存温度の表記について： RT: 室温
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