価格(税別)
¥60,000
在庫
1-9
(2024/03/19 17:00時点)
包装
200 µL (1 mg/mL)
データ | |||||
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クローナリティー | Polyclonal | クローン | Polyclonal | ||
アイソタイプ (免疫動物) |
Rabbit Ig (aff.) | ||||
使用法 | |||||
免疫原(抗原) | KLH conjugated synthetic peptide, corresponding to internal region of human PCBP2 | ||||
交差性 [Gene ID] |
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性状 | 1 mg/mL in PBS/50% glycerol, pH 7.2 | ||||
保存温度 | -20°C | 標識 | Unlabeled | メーカー | MBL |
別称 | poly(rC) binding protein 2, HNRPE2, hnRNP-E2 | ||||
背景 | Poly(rC)-binding proteins (PCBPs) are cellular RNA-binding proteins, characterized by their triple KH structure and by their poly(C)-binding specificity. The 3 KH domains are not only involved in nucleic acid binding but also mediate protein-protein interactions within the RNP complexes. It binds specifically to polypyrimidine tracts within the 3' UTR of target mRNAs through KH domains. PCBPs are expressed as 4 isoforms in cells. PCBP1 and PCBP2 are predominantly expressed in the nucleus and can shuttle from the nucleus to the cytoplasm, while PCBP3 and PCBP4 are expressed to a less extent in the cytoplasm. The most characterized gene regulated by PCBP1 and PCBP2 is the a-globin gene, and the accumulated α-globin mRNA is known to account for approximately 95% of total cellular mRNA during terminal erythroid differentiation. Specific binding of PCBPs to 3′ UTR of mRNA is associated with the stabilization of a-globin mRNA. Microarray analysis of human mRNAs present in a specific subset of PCBP2-RNP complexes also showed a potential involvement of PCBP2 in transcription factor expression via association with their mRNA; further, the enrichment of PCBP2 mRNA in PCBP2-RNP complexes suggested that PCBP2 expression may be subject to posttranscriptional autoregulation. | ||||
関連製品 |
RN1001 RIP-Assay Kit RN1005 RIP-Assay Kit for microRNA RN019P Anti-HNRNPK pAb RN024P Anti-PCBP1 pAb RN011P Anti-PTBP1 (Human) pAb RN052PW Anti-HNRNPC pAb RN060PW Anti-HNRNPD (AUF1) pAb RN080PW Anti-SRSF3 (SRp20) pAb RN004M Anti-Ribosomal P0/P1/P2 mAb RN002P Anti-eIF4G1 (Human) pAb RN114PW Anti-HNRNPA1 pAb |
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使用文献 |
使用文献募集中!本製品を使って論文を発表されましたら是非お知らせください。
Western Blotting
Immunocytochemistry
RNP Immunoprecipitation
Other(Immunodepletion)
Other(eCLIP)
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製品 カテゴリー |
※在庫につきましては2024年03月19日 17時00分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について:
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
※使用法・交差性の表記について:
*: 論文で報告されております(MBLでは未確認)。詳しくはデータシートをご覧ください。
**: 導入元からの情報です(MBLでは評価中もしくは未確認)。
※保存温度の表記について: RT: 室温
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