抗体 製品詳細情報

Code No. RN011P
Anti-PTBP1 (Human) pAb
価格(税別) ¥66,000
包装 200 µL (1 mg/mL)
在庫 10以上
（2024/04/24 17:00時点）
クローナリティー Polyclonal
クローン Polyclonal
アイソタイプ
(免疫動物)		 Rabbit Ig (aff.)
使用法 WB　1:1,000  
IP　5 µg/250 µL of cell extract from 2.5 x 106 cells  
IC*　reported.  （PMID: 24865424 / 26990993）
RIP　15 µg/500 µL of cell extract from 6 x 106 cells  
Other(eCLIP)　reported.  （PMID: 27018577）
免疫原(抗原) KLH-conjugated synthetic peptide KFKGDSRSAGVPSR (46-59 a. a.)
交差性 [Gene ID]

Human[5725], Mouse(-), Rat(-), Hamster(-)
性状 1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度 -20°C
標識 Unlabeled
メーカー MBL
別称 polypyrimidine tract binding protein 1, HNRNP-I, HNRNPI, HNRPI
背景 The polypyrimidine tract binding protein 1 (PTBP1), a 57 kDa RNA binding protein also known as hnRNP1, has been implicated in multiple aspect of mRNA metabolism, including regulation of alternative splicing, internal ribosome entry site-driven translation, mRNA localization, and polyadenylation. Four of tandem RNA recognition motif domains give PTBP1 a strong binding activity to RNA. Posttranscriptional regulation of gene expression by PTBP1 has been reported on many genes, including CD154, insulin, VEGF and Human inducible nitric oxide synthase.
関連製品 RN1001 RIP-Assay Kit
RN1005 RIP-Assay Kit for microRNA
RN052PW Anti-HNRNPC pAb
RN060PW Anti-HNRNPD (AUF1) pAb
RN019P Anti-HNRNPK pAb
RN024P Anti-PCBP1 pAb
RN025P Anti-PCBP2 pAb
RN011P Anti-PTBP1 (Human) pAb
RN114PW Anti-HNRNPA1 pAb
製品カテゴリー 分野:RNA-RNPネットワーク
ブランド:RiboCluster Profiler™
データ



使用文献

Western Blotting

  1. Ferrarese R et al. Lineage-specific splicing of a brain-enriched alternative exon promotes glioblastoma progression. J Clin Invest. 124, 2861-74 (2014)(PMID:24865424)
  2. Liu W et al. Hepatitis B virus core protein inhibits Fas-mediated apoptosis of hepatoma cells via regulation of mFas/FasL and sFas expression. FASEB J. 29, 1113-23 (2015)(PMID:25466893)
  3. Cui J, Placzek WJ. PTBP1 modulation of MCL1 expression regulates cellular apoptosis induced by antitubulin chemotherapeutics. Cell Death Differ. 23, 1681-90 (2016)(PMID:27367564)
  4. Nussbacher JK, Yeo GW. Systematic Discovery of RNA Binding Proteins that Regulate MicroRNA Levels. Mol Cell. 69, 1005-1016.e7 (2018)(PMID:29547715)
  5. Cui J, Placzek WJ. PTBP1 enhances miR-101-guided AGO2 targeting to MCL1 and promotes miR-101-induced apoptosis. Cell Death Dis. 9, 552 (2018)(PMID:29748555)

Immunoprecipitation

  1. Ferrarese R et al. Lineage-specific splicing of a brain-enriched alternative exon promotes glioblastoma progression. J Clin Invest. 124, 2861-74 (2014)(PMID:24865424)

Immunocytochemistry

  1. Ferrarese R et al. Lineage-specific splicing of a brain-enriched alternative exon promotes glioblastoma progression. J Clin Invest. 124, 2861-74 (2014)(PMID:24865424)
  2. Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993)

RNP Immunoprecipitation

  1. Ferrarese R et al. Lineage-specific splicing of a brain-enriched alternative exon promotes glioblastoma progression. J Clin Invest. 124, 2861-74 (2014)(PMID:24865424)
  2. Cui J, Placzek WJ. PTBP1 modulation of MCL1 expression regulates cellular apoptosis induced by antitubulin chemotherapeutics. Cell Death Differ. 23, 1681-90 (2016)(PMID:27367564)
  3. Wang ZN et al. High expression of PTBP1 promote invasion of colorectal cancer by alternative splicing of cortactin. Oncotarget 8, 36185-36202 (2017)(PMID:28404950)

Other(eCLIP)

  1. Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993)
  2. Van Nostrand EL et al. Robust transcriptome-wide discovery of RNA-binding protein binding sites with enhanced CLIP (eCLIP). Nat Methods. 13, 508-14 (2016)(PMID:27018577)

※在庫につきましては2024年04月24日 17時00分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation,
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
※使用法・交差性の表記について：
*: 論文で報告されております（MBLでは未確認）。詳しくはデータシートをご覧ください。
**: 導入元からの情報です（MBLでは評価中もしくは未確認）。
※保存温度の表記について： RT: 室温
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