トップ > 製品検索結果一覧 > Code No. PM036 Anti-LC3 pAb

Code No. PM036

Anti-LC3 pAb

価格(税別)

¥48,000

在庫

10以上

(2017/08/22 01:30時点)

包装

100 µL

データ
  • Western Blotting

  • Western Blotting

  • Immunoprecipitation

  • Immunocytochemistry

  • Immunohistochemistry

クローナリティー Polyclonal クローン Polyclonal
アイソタイプ
(免疫動物)
Rabbit IgG
使用法
WB
1:1,000  
IP
2 µL/300 µL of cell extract from 1x107 cells  
FCM
1:200  
IC
1:500-1:1,000  
IH
1:1,000-1:2,000  
免疫原(抗原) recombinant Human LC3 (MAP1LC3B:1-120 a.a.)
交差性
[Gene ID]

Human[81631/84557/440738], Mouse[67443/66734], Rat[64862/362245], Hamster

性状 PBS/50% glycerol, pH 7.2
保存温度 -20°C 標識 Unlabeled メーカー MBL
別称 microtubule-associated protein 1 light chain 3 beta; microtubule-associated protein 1 light chain 3 alpha; microtubule-associated protein 1 light chain 3 gamma; MAP1LC3B, LC3B, ATG8F, MAP1LC3B-a, MAP1
背景 Macroautophagy mediates the bulk degradation of cytoplasmic components. These components
are delivered to lysosomes via autophagosomes. The microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I. A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II. This antibody can detect both forms of LC3.
関連製品 PM036-PN Positive control for anti-LC3 antibody
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PM045 Anti-p62 (SQSTM1) pAb
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PM066 Anti-p62 C-terminal pAb
PD017 Anti-Beclin 1 pAb
PM037 Anti-GABARAP pAb
M135-3 Anti-GABARAP mAb
PM038 Anti-GATE-16 pAb
PM039 Anti-Atg7 (Human) pAb
PM034 Anti-Atg3 pAb
M133-3 Anti-Atg3 mAb
8485 Autophagy Ab Sampler Set
M152-3MS Anti-LC3 mAb for My select sampler set
PM040MS Anti-Atg16L pAb for My select sampler set
M200-3MS Anti-NRF2 mAb for My select sampler set
M153-3 Anti-Atg5 mAb
M150-3 Anti-Atg16L mAb
8486 Autophagy Watch
M134-3 Anti-Atg4B mAb
M150-3MS Anti-Atg16L mAb for My select sampler set
M154-3 Anti-Atg12 (Human) mAb
M162-A48 Anti-p62 (SQSTM1) (Human) mAb-Alexa Fluor® 488
M162-A59 Anti-p62 (SQSTM1) (Human) mAb-Alexa Fluor® 594
M162-A64 Anti-p62 (SQSTM1) (Human) mAb-Alexa Fluor® 647
M186-7 Anti-LC3 mAb-HRP-DirecT
M186-7MS Anti-LC3 mAb-HRP-DirecT for My select sampler set
M200-3 Anti-NRF2 mAb
PM040 Anti-Atg16L pAb
PM050 Anti-Atg5 pAb
備考 FAQ:よくあるお問い合わせを見る→

 

 

使用文献
使用文献募集中!本製品を使って論文を発表されましたら是非お知らせください。

Western Blotting

  1. Saitoh T et al. Loss of the autophagy protein Atg16L1 enhances endotoxin-induced IL-1beta production. Nature 456, 264-8 (2008)(PMID:18849965)
  2. Katona I et al. Distinct pathogenic processes between Fig4-deficient motor and sensory neurons. Eur J Neurosci. 33, 1401-10 (2011)(PMID:21410794)
  3. Lee EJ, Tournier C. The requirement of uncoordinated 51-like kinase 1 (ULK1) and ULK2 in the regulation of autophagy. Autophagy. 7, 689-95 (2011)(PMID:21460635)
  4. Pan JA et al. Inhibition of protein degradation induces apoptosis through a microtubule-associated protein 1 light chain 3-mediated activation of caspase-8 at intracellular membranes. Mol Cell Biol. 31, 3158-70 (2011)(PMID:21628531)
  5. Huang Y et al. PML-RARα enhances constitutive autophagic activity through inhibiting the Akt/mTOR pathway. Autophagy 7, 1132-44 (2011)(PMID:21673516)
  6. Miranda S et al. Beneficial effects of fenofibrate in retinal pigment epithelium by the modulation of stress and survival signaling under diabetic conditions. J Cell Physiol. 227, 2352-62 (2012)(PMID:21826649)
  7. Otomo T et al. Lysosomal storage causes cellular dysfunction in mucolipidosis II skin fibroblasts. J Biol Chem. 286, 35283-90 (2011)(PMID:21846724)
  8. Yan J et al. Methyl-β-cyclodextrin induces programmed cell death in chronic myeloid leukemia cells and, combined with imatinib, produces a synergistic downregulation of ERK/SPK1 signaling. Anticancer Drugs. 23, 22-31 (2012)(PMID:21857502)
  9. Takaesu G et al. TGFβ-activated kinase 1 (TAK1)-binding proteins (TAB) 2 and 3 negatively regulate autophagy. J Biochem. 151, 157-66 (2012)(PMID:21976705)
  10. Taguwa S et al. Dysfunction of autophagy participates in vacuole formation and cell death in cells replicating hepatitis C virus. J Virol. 85, 13185-94 (2011)(PMID:21994453)
  11. Xu T et al. Modulation of autophagic activity by extracellular pH. Autophagy 7, 1316-22 (2011)(PMID:21997366)
  12. Rasmussen SB et al. Activation of autophagy by α-herpesviruses in myeloid cells is mediated by cytoplasmic viral DNA through a mechanism dependent on stimulator of IFN genes. J Immunol. 187, 5268-76 (2011)(PMID:21998456)
  13. Wu SY et al. Ras-related tumorigenesis is suppressed by BNIP3-mediated autophagy through inhibition of cell proliferation. Neoplasia 13, 1171-82 (2011)(PMID:22241963)
  14. Starr T et al. Selective subversion of autophagy complexes facilitates completion of the Brucella intracellular cycle. Cell Host Microbe. 11, 33-45 (2012)(PMID:22264511)
  15. Kaini RR et al. Autophagy regulates lipolysis and cell survival through lipid droplet degradation in androgen-sensitive prostate cancer cells. Prostate 72, 1412-22 (2012)(PMID:22294520)
  16. Yu X et al. Differential degradation of full-length and cleaved ataxin-7 fragments in a novel stable inducible SCA7 model. J Mol Neurosci. 47, 219-33 (2012)(PMID:22367614)
  17. Niizuma S et al. Effect of persistent activation of phosphoinositide 3-kinase on heart. Life Sci. 90, 619-28 (2012)(PMID:22391413)
  18. Kaminskyy VO et al. Suppression of basal autophagy reduces lung cancer cell proliferation and enhances caspase-dependent and -independent apoptosis by stimulating ROS formation. Autophagy 8, 1032-44 (2012)(PMID:22562073)
  19. Guo JL et al. The Dynamics and Turnover of Tau Aggregates in Cultured Cells: INSIGHTS INTO THERAPIES FOR TAUOPATHIES. J Biol Chem. 291, 13175-93 (2016)(PMID:27129267)
  20. Sano O et al. Vacuolin-1 inhibits autophagy by impairing lysosomal maturation via PIKfyve inhibition. FEBS Lett. 590, 1576-85 (2016)(PMID:27135648)

Immunocytochemistry

  1. Wu YN et al. The selective growth inhibition of oral cancer by iron core-gold shell nanoparticles through mitochondria-mediated autophagy. Biomaterials. 32, 4565-73 (2011)(PMID:21458061)
  2. Yan J et al. Methyl-β-cyclodextrin induces programmed cell death in chronic myeloid leukemia cells and, combined with imatinib, produces a synergistic downregulation of ERK/SPK1 signaling. Anticancer Drugs. 23, 22-31 (2012)(PMID:21857502)
  3. Taguwa S et al. Dysfunction of autophagy participates in vacuole formation and cell death in cells replicating hepatitis C virus. J Virol. 85, 13185-94 (2011)(PMID:21994453)
  4. Rasmussen SB et al. Activation of autophagy by α-herpesviruses in myeloid cells is mediated by cytoplasmic viral DNA through a mechanism dependent on stimulator of IFN genes. J Immunol. 187, 5268-76 (2011)(PMID:21998456)
  5. Starr T et al. Selective subversion of autophagy complexes facilitates completion of the Brucella intracellular cycle. Cell Host Microbe. 11, 33-45 (2012)(PMID:22264511)
  6. Seillier M et al. TP53INP1, a tumor suppressor, interacts with LC3 and ATG8-family proteins through the LC3-interacting region (LIR) and promotes autophagy-dependent cell death. Cell Death Differ. 19, 1525-35 (2012)(PMID:22421968)
  7. Kaminskyy VO et al. Suppression of basal autophagy reduces lung cancer cell proliferation and enhances caspase-dependent and -independent apoptosis by stimulating ROS formation. Autophagy 8, 1032-44 (2012)(PMID:22562073)
  8. Guo JL et al. The Dynamics and Turnover of Tau Aggregates in Cultured Cells: INSIGHTS INTO THERAPIES FOR TAUOPATHIES. J Biol Chem. 291, 13175-93 (2016)(PMID:27129267)
  9. Sano O et al. Vacuolin-1 inhibits autophagy by impairing lysosomal maturation via PIKfyve inhibition. FEBS Lett. 590, 1576-85 (2016)(PMID:27135648)

Immunohistochemistry

  1. Hasui K et al. Enhanced Autophagy and Reduced Expression of Cathepsin D Are Related to Autophagic Cell Death in Epstein-Barr Virus-Associated Nasal Natural Killer/T-Cell Lymphomas: An Immunohistochemical Analysis of Beclin-1, LC3, Mitochondria (AE-1), and Cathepsin D in Nasopharyngeal Lymphomas. Acta Histochem Cytochem. 44, 119-31 (2011)(PMID:21753858)
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※在庫につきましては2017年08月22日 01時30分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について:
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
※使用法・交差性の表記について:
*: 論文で報告されております(MBLでは未確認)。詳しくはデータシートをご覧ください。
**: 導入元からの情報です(MBLでは評価中もしくは未確認)。
※保存温度の表記について: RT: 室温
※本サイトの掲載製品は、品質改良のため予告なく仕様などの変更、または販売中止となる場合がございます。あらかじめご了承ください。