Anti-Atg7 (Human) pAb

価格（税別）

¥41,800

在庫

1-9

（2024/04/19 00:05時点）

包装

100 µL

使用文献

データシート

SDS

データ	Western Blotting Immunoprecipitation
クローナリティー	Polyclonal	クローン	Polyclonal
アイソタイプ（免疫動物）	Rabbit Ig (aff.)
使用法	WB 1:1,000-1:2,000 IP 5 µL/300 µL of cell extract from 3x10⁶ cells
免疫原(抗原)	Human Atg7 C-terminal synthetic peptide
交差性 [Gene ID]	Human[10533], Mouse(-), Rat(-), Hamster(-)
性状	PBS/50% glycerol, pH 7.2
保存温度	-20°C	標識	Unlabeled	メーカー	MBL
別称	autophagy related 7, GSA7, APG7L, APG7-LIKE
背景	Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. Microtubule-associated protein light chain 3 (LC3) is a homologue of yeast Atg8, an essential component of autophagy. Following synthesis, the C-terminus of LC3 is cleaved by a cysteine protease-Atg4, to produce LC3-I, which is located in cytosolic fraction. LC3-I is activated by the E1-like enzyme Atg7 and forms a Atg7-LC3-I thioester. Atg7-LC3-I is transferred to Atg3 to form Atg3-LC3-I thioester. Atg3 is an E2-like enzyme that catalyzes the conjugation of LC3-I and phosphatidylethanolamine (PE) to form LC3-II. The LC3-II-PE conjugate is essential for binding tightly to autophagosomal membrane.
関連製品	M133-3 Anti-Atg3 mAb PM050 Anti-Atg5 pAb M153-3 Anti-Atg5 mAb PM039 Anti-Atg7 (Human) pAb PM036 Anti-LC3 pAb M152-3 Anti-LC3 mAb PD042 Anti-Atg9A pAb M134-3 Anti-Atg4B mAb M154-3 Anti-Atg12 (Human) mAb PM040 Anti-Atg16L pAb M150-3 Anti-Atg16L mAb PM072 Anti-VMP1 pAb PM090 Anti-Atg8 (Filamentous fungi) pAb M152-3MS Anti-LC3 mAb for My select sampler set PM037 Anti-GABARAP pAb M135-3 Anti-GABARAP mAb M151-3 Anti-Atg10 (Human) mAb M186-3 Anti-LC3 mAb M200-3 Anti-NRF2 mAb PD014 Anti-LC3 pAb PM069 Anti-NRF2 pAb
使用文献	使用文献募集中！本製品を使って論文を発表されましたら是非お知らせください。 Western Blotting Fujita K et al. Nrf2-mediated induction of p62 controls Toll-like receptor-4-driven aggresome-like induced structure formation and autophagic degradation. PNAS. 108, 1427-32 (2011)(PMID:21220332) Maejima Y et al. Mst1 inhibits autophagy by promoting the interaction between Beclin1 and Bcl-2. Nat Med. 19, 1478-88 (2013)(PMID:24141421) Horikawa I et al. Autophagic degradation of the inhibitory p53 isoform Δ133p53α as a regulatory mechanism for p53-mediated senescence. Nat Commun. 5, 4706 (2014)(PMID:25144556) Xu D et al. Modification of BECN1 by ISG15 plays a crucial role in autophagy regulation by type I IFN/interferon. Autophagy 11, 617-28 (2015)(PMID:25906440) He R et al. Divergent roles of BECN1 in LC3 lipidation and autophagosomal function. Autophagy 11, 740-7 (2015)(PMID:25955014) Dutta P et al. MIR494 reduces renal cancer cell survival coinciding with increased lipid droplets and mitochondrial changes. BMC Cancer 16, 33 (2016)(PMID:26794413) Kim HJ et al. Subcellular localization of FOXO3a as a potential biomarker of response to combined treatment with inhibitors of PI3K and autophagy in PIK3CA-mutant cancer cells. Oncotarget. 8, 6608-6622 (2017)(PMID:28036259) He R et al. Glucosyltransferase Activity of Clostridium difficile Toxin B Triggers Autophagy-mediated Cell Growth Arrest. Sci Rep. 7, 10532 (2017)(PMID:28874882) Liu WJ et al. Lysosome restoration to activate podocyte autophagy: a new therapeutic strategy for diabetic kidney disease. Cell Death Dis. 10, 806 (2019)(PMID:31649253) Chhabra R, Nanjundan M. Lysophosphatidic acid reverses Temsirolimus-induced changes in lipid droplets and mitochondrial networks in renal cancer cells. PLoS One.15, e0233887 (2020)(PMID:32492043) Immunofluorescence Liu WJ et al. Lysosome restoration to activate podocyte autophagy: a new therapeutic strategy for diabetic kidney disease. Cell Death Dis. 10, 806 (2019)(PMID:31649253)
製品カテゴリー	分野オートファジー

※在庫につきましては2024年04月19日 00時05分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
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※使用法の表記について：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
※使用法・交差性の表記について：
*: 論文で報告されております（MBLでは未確認）。詳しくはデータシートをご覧ください。
**: 導入元からの情報です（MBLでは評価中もしくは未確認）。
※保存温度の表記について： RT: 室温
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