| データ |
 Western Blotting
|
| クローナリティー |
Polyclonal
|
クローン |
Polyclonal |
アイソタイプ
（免疫動物）
|
Rabbit Ig (aff.)
|
| 使用法 |
- WB
- 1:1,000
 - IP
- 5 µL/sample
- IC*
- reported. （PMID: 21216843）
|
| 免疫原(抗原) |
Carrier protein conjugated 6xHissynthetic peptide
|
| 性状 |
PBS/50% glycerol, pH 7.2
|
| 保存温度 |
-20°C
|
標識 |
Unlabeled |
メーカー |
MBL |
| 背景 |
Expression vectors containing a protein and a tag peptide are commonly used. His-tag fusion protein expression system is preferably used in various laboratories, because its simple protein purification step by heavy metal, such as nickel, affinity chromatography. This specific antibody for His-tag fusion protein is useful tool for monitoring of the fusion protein expression and affinity purification.
|
| 関連製品 |
PM032-8 Anti-His-tag pAb-Agarose
D291-3 Anti-His-tag mAb
D291-6 Anti-His-tag mAb-Biotin
D291-7 Anti-His-tag mAb-HRP-DirecT
D291-8 Anti-His-tag mAb-Agarose
D291-10 Anti-His-tag mAb-Magnetic Agarose
D291-A48 Anti-His-tag mAb-Alexa Fluor™ 488
D291-A59 Anti-His-tag mAb-Alexa Fluor™ 594
D291-A64 Anti-His-tag mAb-Alexa Fluor™ 647
M089-3 Anti-His-tag mAb
M136-3 Anti-His-tag mAb
D291-11 Anti-His-tag mAb-Magnetic Beads
|
使用文献
|
使用文献募集中！本製品を使って論文を発表されましたら是非お知らせください。
Western Blotting - Shibata Y et al. Activation of the IκEB kinase complex by HTLV-1 Tax requires cytosolic factors involved in Tax-induced polyubiquitination. J Biochem. 150, 679-86 (2011)(PMID:21862596)
- Kajikawa M et al. The intertransmembrane region of Kaposi's sarcoma-associated herpesvirus modulator of immune recognition 2 contributes to B7-2 downregulation. J Virol. 86, 5288-96 (2012)(PMID:22379101)
- Nishiyama A et al. Uhrf1-dependent H3K23 ubiquitylation couples maintenance DNA methylation and replication. Nature. 502, 249-53 (2013)(PMID:24013172)
- Lee Y et al. Structure of the triose-phosphate/phosphate translocator reveals the basis of substrate specificity. Nat Plants. 3, 825-832 (2017)(PMID:28970497)
- Li Z et al. Destabilization of linker histone H1.2 is essential for ATM activation and DNA damage repair. Cell Res. 28, 756-770 (2018)(PMID:29844578)
- Fukui R et al. Cleavage of Toll-Like Receptor 9 Ectodomain Is Required for In Vivo Responses to Single Strand DNA. Front Immunol. 9, 1491 (2018)(PMID:29997629)
- Kang J et al. FIH permits NAA10 to catalyze the oxygen-dependent lysyl-acetylation of HIF-1α. Redox Biol. 19, 364-374 (2018)(PMID:30237125)
- Li M et al. p53 cooperates with SIRT6 to regulate cardiolipin de novo biosynthesis. Cell Death Dis. 9, 941 (2018)(PMID:30237540)
- Gao T et al. PKCζ Phosphorylates SIRT6 to Mediate Fatty Acid β-Oxidation in Colon Cancer Cells. Neoplasia. 21, 61-73 (2019)(PMID:30504065)
- Kim J et al. Ketohexokinase-A acts as a nuclear protein kinase that mediates fructose-induced metastasis in breast cancer. Nat Commun. 11, 5436 (2020)(PMID:33116123)
Immunoprecipitation - Kojima K et al. Associations between PIWI proteins and TDRD1/MTR-1 are critical for integrated subcellular localization in murine male germ cells. Genes Cells. 14, 1155-65 (2009)(PMID:19735482)
- Zhang L et al. POH1 deubiquitinates pro-interleukin-1β and restricts inflammasome activity. Nat Commun. 9, 4225 (2018)(PMID:30315153)
Immunocytochemistry - Takemoto M et al. Laminar and areal expression of unc5d and its role in cortical cell survival. Cereb Cortex. 21, 1925-34 (2011)(PMID:21216843)
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製品
カテゴリー |
- ツール
- エピトープタグ
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※在庫につきましては2024年04月19日 00時05分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
※使用法・交差性の表記について：
*: 論文で報告されております（MBLでは未確認）。詳しくはデータシートをご覧ください。
**: 導入元からの情報です（MBLでは評価中もしくは未確認）。
※保存温度の表記について： RT: 室温
※本サイトの掲載製品は、品質改良のため予告なく仕様などの変更、または販売中止となる場合がございます。あらかじめご了承ください。
使用文献
データシート