抗体 製品詳細情報

Code No. PM032 プリントプリント
Anti-His-tag pAb
価格(税別) ¥40,000
包装 100 µL
在庫 10以上
(2019/12/08 17:00時点)
クローナリティー Polyclonal
クローン Polyclonal
アイソタイプ
(免疫動物)
Rabbit Ig (aff.)
使用法 WB 1:1,000  
IP 5 µL/sample  
IC* reported.  (PMID: 21216843)
免疫原(抗原) Carrier protein conjugated 6xHissynthetic peptide
性状 PBS/50% glycerol, pH 7.2
保存温度 -20°C
標識 Unlabeled
メーカー MBL
背景 Expression vectors containing a protein and a tag peptide are commonly used. His-tag fusion protein expression system is preferably used in various laboratories, because its simple protein purification step by heavy metal, such as nickel, affinity chromatography. This specific antibody for His-tag fusion protein is useful tool for monitoring of the fusion protein expression and affinity purification.
関連製品 PM032-8 Anti-His-tag pAb-Agarose
D291-3 Anti-His-tag mAb
D291-3S Anti-His-tag mAb
D291-6 Anti-His-tag mAb-Biotin
D291-7 Anti-His-tag mAb-HRP-DirecT
D291-8 Anti-His-tag mAb-Agarose
D291-10 Anti-His-tag mAb-Magnetic Agarose
D291-A48 Anti-His-tag mAb-Alexa Fluor® 488
D291-A59 Anti-His-tag mAb-Alexa Fluor® 594
D291-A64 Anti-His-tag mAb-Alexa Fluor® 647
M089-3 Anti-His-tag mAb
M136-3 Anti-His-tag mAb
D291-11 Anti-His-tag mAb-Magnetic Beads
製品カテゴリー ツール:エピトープタグ
ブランド:使用文献・データ募集プレゼント対象製品
データ

使用文献

Western Blotting

  1. Shibata Y et al. Activation of the IκEB kinase complex by HTLV-1 Tax requires cytosolic factors involved in Tax-induced polyubiquitination. J Biochem. 150, 679-86 (2011)(PMID:21862596)
  2. Kajikawa M et al. The intertransmembrane region of Kaposi's sarcoma-associated herpesvirus modulator of immune recognition 2 contributes to B7-2 downregulation. J Virol. 86, 5288-96 (2012)(PMID:22379101)
  3. Nishiyama A et al. Uhrf1-dependent H3K23 ubiquitylation couples maintenance DNA methylation and replication. Nature. 502, 249-53 (2013)(PMID:24013172)
  4. Lee Y et al. Structure of the triose-phosphate/phosphate translocator reveals the basis of substrate specificity. Nat Plants. 3, 825-832 (2017)(PMID:28970497)
  5. Li Z et al. Destabilization of linker histone H1.2 is essential for ATM activation and DNA damage repair. Cell Res. 28, 756-770 (2018)(PMID:29844578)
  6. Fukui R et al. Cleavage of Toll-Like Receptor 9 Ectodomain Is Required for In Vivo Responses to Single Strand DNA. Front Immunol. 9, 1491 (2018)(PMID:29997629)
  7. Kang J et al. FIH permits NAA10 to catalyze the oxygen-dependent lysyl-acetylation of HIF-1α. Redox Biol. 19, 364-374 (2018)(PMID:30237125)
  8. Li M et al. p53 cooperates with SIRT6 to regulate cardiolipin de novo biosynthesis. Cell Death Dis. 9, 941 (2018)(PMID:30237540)
  9. Gao T et al. PKCζ Phosphorylates SIRT6 to Mediate Fatty Acid β-Oxidation in Colon Cancer Cells. Neoplasia. 21, 61-73 (2019)(PMID:30504065)

Immunoprecipitation

  1. Kojima K et al. Associations between PIWI proteins and TDRD1/MTR-1 are critical for integrated subcellular localization in murine male germ cells. Genes Cells. 14, 1155-65 (2009)(PMID:19735482)
  2. Zhang L et al. POH1 deubiquitinates pro-interleukin-1β and restricts inflammasome activity. Nat Commun. 9, 4225 (2018)(PMID:30315153)

Immunocytochemistry

  1. Takemoto M et al. Laminar and areal expression of unc5d and its role in cortical cell survival. Cereb Cortex. 21, 1925-34 (2011)(PMID:21216843)

※在庫につきましては2019年12月08日 17時00分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について:
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation,
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization

ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
※使用法・交差性の表記について:
*: 論文で報告されております(MBLでは未確認)。詳しくはデータシートをご覧ください。
**: 導入元からの情報です(MBLでは評価中もしくは未確認)。
※保存温度の表記について: RT: 室温
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