Recombinant Human R-spondin 1

　

■ What is R-spondin 1?
■ Product Usage Example
　・Human intestine organoid culture
■ Product Summary
■ References

R-spondin 1 (roof plate-specific spondin 1), also known as Cristin 3, is one of the R-spondin family members that regulates to the activation of canonical Wnt/β-catenin signaling. In the canonical Wnt/β-catenin signaling pathway, when Wnt binds to cell surface receptors, Fz^*1 and LRP5/6^*2, intracellular β-catenin accumulates and activates the β-catenin pathway. However,in the absence of R-spondin, Fz is endocytosed by being ubiquitinated by the binding of the ubiquitin ligase RNF43^*3/ZNRF3^*4, Fz on the cell membrane decreases, and Wnt/β-catenin signals are not activated enough. Conversely, in the presence of R-spondin, RNF43/ZNRF3 forms trimers with LGRs^*5 and R-spondin and is endocytosed, making it unable to ubiquitinate Fz. As a result, Fz on the cell membrane increases, and Wnt/β-catenin signals are strongly activated.¹⁾

Organoid culture is typically utilized niche factors that act on Wnt, EGF, BMP and Notch signals that control stem cell differentiation. Among these signals, R-spondin 1 contributes to the activation of Wnt/β-catenin signals as described above.

MBL’s Recombinant Human R-spondin 1 (Code No. J2-004) is a protein derived from the gene encoding human R-spondin 1 (region; 21-146 aa) expressed in E. coli. This region contains a bioactive fragment comprising the two cysteine-rich furin-like domains (Fu1 and Fu2). Fu1 binds to the extracellular domain of ZNRF3 and RNF43, and Fu2 binds to the extracellular domain of LGR4/5/6. ²⁾

*1 Frizzled, *2 low-density-lipoprotein receptor-related protein 5/6, *3 ring finger protein 43, *4 zinc and ring finger 3, *5 leucine-rich repeat-containing G-protein coupled receptors




adapted from Kikuchi, A. et al. Seikagaku. 2020

Human intestine organoid culture

Human small intestine organoids were cultured in the presence of Rrecombinant Human R-spondin 1 [J2-004]. As a result, it was confirmed that human small intestine organoids could be cultured under conditions in which MBL’s R-spondin 1 was added at a concentration of 10 to 1000 ng/mL.

Code No.	Product name	Source	Accession No.	Form	Size
J2-004	Recombinant Human R-spondin 1	21-146 aa	Q2MKA7	Lyophilized from PBS	50 µg

1) Kikuchi, A. et al. Seikagaku. 2020
2) Gui-Xun, Shi. et al. Prog Biophys Mol Biol. 2016 [PMID: 27237581 ]

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