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HOME > Product search results > Code No. PM040 Anti-Atg16L pAb

Code No. PM040

Anti-Atg16L pAb

Price

¥41,800

Availability (in Japan)

10 or more

(In Japan at 17:00,
Apr 16, 2024 in JST)

Size

100 µL

Data
  • Western Blotting

  • Immunoprecipitation

  • Immunocytochemistry

Clonality Polyclonal Clone Polyclonal
Isotype (Immunized Animal) Rabbit Ig (aff.)
Applications
WB
1:1,000  
IP
2.5 µL/300 µL of cell extract from 3x106 cells  
IC
1:200-1:500  
Other(Image-based flow cytometry)
reported.  (PMID: 24553140
Immunogen (Antigen) Recombinant Human ATG16L1 TV2 (85-588 a.a.)
Reactivity [Gene ID]

Human[55054], Mouse[77040], Rat[363278], Hamster[100771530]

Storage buffer PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names ATG16L1, autophagy related 16-like 1 (S. cerevisiae), IBD10, WDR30, APG16L, ATG16A, Apg16l
Background Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. Autophagy has two ubiquitin-like conjugation systems, the Atg12 and LC3-II systems. In the Atg12 conjugation system, the Atg16L-Atg12-Atg5 forms 800 kDa complex that elongate autophagic isolation membrane. After completion of the formation of the autophagosome, the Atg12-Atg5-Atg16L complex dissociates from the membrane. In recent study, nonsynonymous SNP analysis has indicated that ATG16L1 is a Crohn's disease susceptibility gene.
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Citations

Western Blotting

  1. Seto S et al. Autophagy adaptor protein p62/SQSTM1 and autophagy-related gene Atg5 mediate autophagosome formation in response to Mycobacterium tuberculosis infection in dendritic cells. PLoS One 8, e86017 (2013)(PMID:24376899)
  2. Ohshima J et al. Role of mouse and human autophagy proteins in IFN-γ-induced cell-autonomous responses against Toxoplasma gondii. J Immunol. 192, 3328-35 (2014)(PMID:24563254)
  3. Erbil S et al. RACK1 is an interaction partner of ATG5 and a novel regulator of autophagy. J Biol Chem. 291, 16753-65 (2016)(PMID:27325703)
  4. Kimura T et al. Dedicated SNAREs and specialized TRIM cargo receptors mediate secretory autophagy. EMBO J. 36, 42-60 (2017)(PMID:27932448)
  5. Puri C et al. The RAB11A-Positive Compartment Is a Primary Platform for Autophagosome Assembly Mediated by WIPI2 Recognition of PI3P-RAB11A. Dev Cell. 45, 114-131.e8 (2018)(PMID:29634932)
  6. Sorbara MT et al. Complement C3 drives autophagy-dependent restriction of cyto-invasive bacteria. Cell Host Microbe. 23, 644-652.e5 (2018)(PMID:29746835)
  7. Scrivo A et al. Gigaxonin E3 ligase governs ATG16L1 turnover to control autophagosome production. Nat Commun. 10, 780 (2019)(PMID:30770803)
  8. Zemirli N et al. The primary cilium protein folliculin is part of the autophagy signaling pathway to regulate epithelial cell size in response to fluid flow. Cell Stress. 3, 100-109 (2019)(PMID:31225504)
  9. Ding X et al. RAB2 regulates the formation of autophagosome and autolysosome in mammalian cells. Autophagy. 15, 1774-1786 (2019) (PMID:30957628)
  10. Boukhalfa A et al. PI3KC2α-dependent and VPS34-independent Generation of PI3P Controls Primary Cilium-Mediated Autophagy in Response to Shear Stress. Nat Commun. 11, 294 (2020)(PMID:31941925)
  11. Yamamoto YH et al. ERdj8 governs the size of autophagosomes during the formation process. J Cell Biol. 219, e201903127 (2020)(PMID:32492081)
  12. Metje-Sprink J et al. Crystal structure of the Rab33B/Atg16L1 effector complex. Sci Rep. 10, 12956 (2020)(PMID:32737358)

Co-IP

  1. Slowicka K et al. Physical and functional interaction between A20 and ATG16L1-WD40 domain in the control of intestinal homeostasis. Nat Commun. 10, 1834 (2019)(PMID:31015422)

Immunoprecipitation

  1. Murthy A et al. A Crohn's disease variant in Atg16l1 enhances its degradation by caspase 3. Nature 506, 456-62 (2014)(PMID:24553140)
  2. Serramito-Gómez I et al. Regulation of cytokine signaling through direct interaction between cytokine receptors and the ATG16L1 WD40 domain. Nat Commun. 11, 5919 (2020) (PMID:33219218)

Immunocytochemistry

  1. Matsunaga K et al. Autophagy requires endoplasmic reticulum targeting of the PI3-kinase complex via Atg14L. J Cell Biol. 190, 511-21 (2010)(PMID:20713597)
  2. Takahashi Y et al. Bif-1 regulates Atg9 trafficking by mediating the fission of Golgi membranes during autophagy. Autophagy 7, 61-73 (2011)(PMID:21068542)
  3. Moreau K et al. Arf6 promotes autophagosome formation via effects on phosphatidylinositol 4,5-bisphosphate and phospholipase D. J Cell Biol. 196, 483-96 (2012)(PMID:22351926)
  4. Young MM et al. Autophagosomal membrane serves as platform for intracellular death-inducing signaling complex (iDISC)-mediated caspase-8 activation and apoptosis. J Biol Chem. 287, 12455-68 (2012)(PMID:22362782)
  5. Sakamoto S et al. Mild MPP+ exposure-induced glucose starvation enhances autophagosome synthesis and impairs its degradation. Sci Rep. 7, 46668 (2017)(PMID:28443637)
  6. Fletcher K et al. The WD40 domain of ATG16L1 is required for its non-canonical role in lipidation of LC3 at single membranes.EMBO J. 37, e97840 (2018)(PMID:29317426)
  7. Sorbara MT et al. Complement C3 drives autophagy-dependent restriction of cyto-invasive bacteria. Cell Host Microbe. 23, 644-652.e5 (2018)(PMID:29746835)
  8. Takahashi Y et al. An autophagy assay reveals the ESCRT-III component CHMP2A as a regulator of phagophore closure. Nat Commun. 9, 2855 (2018)(PMID:30030437)
  9. Pavel M et al. Contact inhibition controls cell survival and proliferation via YAP/TAZ-autophagy axis. Nat Commun. 9, 2961 (2018)(PMID:30054475)
  10. Fahmy AM et al. LC3B is not recruited along with the autophagy elongation complex (ATG5-12/16L1) at HCV replication site and is dispensable for viral replication. PLoS One. 13, e0205189 (2018)(PMID:30286180)
  11. Scrivo A et al. Gigaxonin E3 ligase governs ATG16L1 turnover to control autophagosome production. Nat Commun. 10, 780 (2019)(PMID:30770803)
  12. De Faveri F et al. LAP-like non-canonical autophagy and evolution of endocytic vacuoles in pancreatic acinar cells. Autophagy. in press. (PMID:31651224)
  13. Boukhalfa A et al. PI3KC2α-dependent and VPS34-independent Generation of PI3P Controls Primary Cilium-Mediated Autophagy in Response to Shear Stress. Nat Commun. 11, 294 (2020)(PMID:31941925)
  14. Besemer AS et al. Receptor-mediated endocytosis 8 (RME-8)/DNAJC13 is a novel positive modulator of autophagy and stabilizes cellular protein homeostasis. Cell Mol Life Sci. in press (PMID:32322926)

Immunofluorescence

  1. Zemirli N et al. The primary cilium protein folliculin is part of the autophagy signaling pathway to regulate epithelial cell size in response to fluid flow. Cell Stress. 3, 100-109 (2019)(PMID:31225504)
  2. Tamura N et al. Hyperosmotic Stress Induces Unconventional Autophagy Independent of the Ulk1 Complex. Mol Cell Biol. 39, pii: e00024-19 (2019)(PMID:31160490)

Other(Image-based flow cytometry)

  1. Murthy A et al. A Crohn's disease variant in Atg16l1 enhances its degradation by caspase 3. Nature 506, 456-62 (2014)(PMID:24553140)
Product category
Research area
Autophagy
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  • The availability is based on the information in Japan at 17:00, Apr 16, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.