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HOME > Product search results > Code No. M186-7 Anti-LC3 mAb-HRP-DirecT

Code No. M186-7

Anti-LC3 mAb-HRP-DirecT

Price

¥48,000

Availability (in Japan)

1-9

(In Japan at 00:05,
Nov 22, 2019 in JST)

Size

50 µL

Data
  • Western Blotting

Clonality Monoclonal Clone 8E10
Isotype (Immunized Animal) Mouse IgG2aκ
Applications
WB
1:1,000  
Immunogen (Antigen) Human LC3 (MAP1LC3B), 1-120 aa (recombinant)
Reactivity [Gene ID]

Human[81631], Mouse[67443], Rat[64862], Hamster[100769810]

Storage buffer PBS/Preservative/Stabilizer
Storage temp. 4°C Conjugate HRP Manufacturer MBL
Alternative names microtubule-associated protein 1 light chain 3 beta, LC3B, ATG8F, MAP1LC3B-a, MAP1A/1BLC3, MAP1LC3B
Background Macroautophagy mediates the bulk degradation of cytoplasmic components. These components are delivered to lysosomes via autophagosomes. The microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I (18 kDa). A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II (16 kDa). This antibody can detect both forms of LC3.
Related products 8486 Autophagy Watch
M186-3 Anti-LC3 mAb
PM036 Anti-LC3 pAb
M152-3 Anti-LC3 mAb
PD014 Anti-LC3 pAb
8485 Autophagy Ab Sampler Set
PM036-PN Positive control for anti-LC3 antibody
Notes Clone 8E10 reacts with LC3B and does not cross-react with LC3A, LC3C, GATE-16, and GABARAP.
Citations

Western Blotting

  1. Ohmae S et al. Actin-binding protein coronin 1A controls osteoclastic bone resorption by regulating lysosomal
    secretion of cathepsin K. Sci Rep. 7, 41710 (2017)(PMID:28300073)
  2. Mori H et al. Induction of selective autophagy in cells replicating hepatitis C virus genome. J Gen Virol. 99, 1643-1657 (2018)(PMID:30311874)
References
  1. Jia W et al. Temporal regulation of intracellular organelle homeostasis in T lymphocytes by autophagy. J Immunol. 186, 5313-22 (2011)
  2. Tabata K et al. Rubicon and PLEKHM1 negatively regulate the endocytic/autophagic pathway via a novel Rab7-binding domain. Mol Biol Cell 21, 4162-72 (2010)
  3. Mookerjee S et al. Posttranslational modification of ataxin-7 at lysine 257 prevents autophagy-mediated turnover of an N-terminal caspase-7 cleavage fragment. J Neurosci. 29, 15134-44 (2009)
  4. Saitoh T et al. Loss of the autophagy protein Atg16L1 enhances endotoxin-induced IL-1beta production. Nature 456, 264-8 (2008)
  5. Kabeya Y et al. LC3, GABARAP and GATE16 localize to autophagosomal membrane depending on form-II formation. J Cell Sci. 117, 2805-12 (2004)
  6. Mizushima N et al. In vivo analysis of autophagy in response to nutrient starvation using transgenic mice expressing a fluorescent autophagosome marker. Mol Biol Cell 15, 1101-11 (2004)
  7. Mizushima N et al. Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells. J Cell Biol. 152, 657-68 (2001)
Product category
Research area
Autophagy
Brands
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  • The availability is based on the information in Japan at 00:05, Nov 22, 2019 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.