Antibody detailed product information

Code No.	M186-7
Anti-LC3 mAb-HRP-DirecT
Price	¥52,800
Size	50 µL
Availability (in Japan)	1-9 （In Japan at 00:05, Mar 23, 2025 in JST）
Clonality	Monoclonal
Clone	8E10
Isotype (Immunized Animal)	Mouse IgG2aκ
Applications	WB　1:1,000
Immunogen (Antigen)	Human LC3 (MAP1LC3B), 1-120 aa (recombinant)
Reactivity [Gene ID]	Human[81631], Mouse[67443], Rat[64862], Hamster[100769810]
Storage buffer	PBS/Preservative/Stabilizer
Storage temp.	4°C
Conjugate	HRP
Manufacturer	MBL
Alternative names	microtubule-associated protein 1 light chain 3 beta, LC3B, ATG8F, MAP1LC3B-a, MAP1A/1BLC3, MAP1LC3B
Background	Macroautophagy mediates the bulk degradation of cytoplasmic components. These components are delivered to lysosomes via autophagosomes. The microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I (18 kDa). A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II (16 kDa). This antibody can detect both forms of LC3.
Related products	8486 Autophagy Watch M186-3 Anti-LC3 mAb PM036 Anti-LC3 pAb M152-3 Anti-LC3 mAb PD014 Anti-LC3 pAb 8485 Autophagy Ab Sampler Set PM036-PN Positive control for anti-LC3 antibody
Notes	Clone 8E10 reacts with LC3B and does not cross-react with LC3A, LC3C, GATE-16, and GABARAP.
Product category	Research area:Autophagy
Data
Citations	Western Blotting Ohmae S et al. Actin-binding protein coronin 1A controls osteoclastic bone resorption by regulating lysosomal secretion of cathepsin K. Sci Rep. 7, 41710 (2017)(PMID:28300073) Mori H et al. Induction of selective autophagy in cells replicating hepatitis C virus genome. J Gen Virol. 99, 1643-1657 (2018)(PMID:30311874)
References	Jia W et al. Temporal regulation of intracellular organelle homeostasis in T lymphocytes by autophagy. J Immunol. 186, 5313-22 (2011) Tabata K et al. Rubicon and PLEKHM1 negatively regulate the endocytic/autophagic pathway via a novel Rab7-binding domain. Mol Biol Cell 21, 4162-72 (2010) Mookerjee S et al. Posttranslational modification of ataxin-7 at lysine 257 prevents autophagy-mediated turnover of an N-terminal caspase-7 cleavage fragment. J Neurosci. 29, 15134-44 (2009) Saitoh T et al. Loss of the autophagy protein Atg16L1 enhances endotoxin-induced IL-1beta production. Nature 456, 264-8 (2008) Kabeya Y et al. LC3, GABARAP and GATE16 localize to autophagosomal membrane depending on form-II formation. J Cell Sci. 117, 2805-12 (2004) Mizushima N et al. In vivo analysis of autophagy in response to nutrient starvation using transgenic mice expressing a fluorescent autophagosome marker. Mol Biol Cell 15, 1101-11 (2004) Mizushima N et al. Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells. J Cell Biol. 152, 657-68 (2001)

The availability is based on the information in Japan at 00:05, Mar 23, 2025 in JST.
The special price is shown in red color.
Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.