Data |
Western Blotting

|
Clonality |
Monoclonal
|
Clone |
4D3 |
Isotype
(Immunized Animal)
|
Mouse IgG1 κ
|
Applications |
- WB
- 2-5 µg/mL

|
Immunogen
(Antigen) |
Recombinant Human full-length Atg5 (275 a.a.)
|
Reactivity
[Gene ID] |
Human[9474], Mouse[11793], Rat(-), Hamster[100759159] |
Storage buffer |
PBS/50% glycerol, pH 7.2
|
Storage temp. |
-20°C
|
Conjugate |
Unlabeled |
Manufacturer |
MBL |
Alternative names |
autophagy related 5, ASP, APG5, APG5L, hAPG5, APG5-LIKE
|
Related products |
8485 Autophagy Ab Sampler Set PM050 Anti-Atg5 pAb M133-3 Anti-Atg3 mAb PM039 Anti-Atg7 (Human) pAb PM036 Anti-LC3 pAb M152-3 Anti-LC3 mAb M151-3 Anti-Atg10 (Human) mAb M154-3 Anti-Atg12 (Human) mAb PM040 Anti-Atg16L pAb M150-3 Anti-Atg16L mAb PD017 Anti-Beclin 1 pAb PM072 Anti-VMP1 pAb
|
Notes |
This antibody detects Atg5-Atg12 complex (55 kDa).
|
Citations |
Western Blotting - Takahashi Y et al. Bif-1 regulates Atg9 trafficking by mediating the fission of Golgi membranes during autophagy. Autophagy. 7, 61-73 (2011)(PMID:21068542)
- Takaesu G et al. TGFβ-activated kinase 1 (TAK1)-binding proteins (TAB) 2 and 3 negatively regulate autophagy. J Biochem. 151, 157-66 (2012)(PMID:21976705)
- Young MM et al. Autophagosomal membrane serves as platform for intracellular death-inducing signaling complex (iDISC)-mediated caspase-8 activation and apoptosis. J Biol Chem. 287, 12455-68 (2012)(PMID:22362782)
- Chen H et al. CD133/prominin-1-mediated autophagy and glucose uptake beneficial for hepatoma cell survival. PLoS One 8, e56878 (2013)(PMID:23437259)
- Guo X et al. Patulin induces pro-survival functions via autophagy inhibition and p62 accumulation. Cell Death Dis. 4, e822 (2013)(PMID:24091665)
- Katagiri N et al. The nucleolar protein nucleophosmin is essential for autophagy induced by inhibiting Pol I transcription. Sci Rep. 5, 8903 (2015)(PMID:25754892)
- Liu Y et al. Bif-1 deficiency impairs lipid homeostasis and causes obesity accompanied by insulin resistance. Sci Rep. 6, 20453 (2016)(PMID:26857140)
- Cheng J et al. Autophagy regulates MAVS signaling activation in a phosphorylation-dependent manner in microglia. Cell Death Differ. 24, 276-287 (2017)(PMID:28141795)
- Ye M et al. Curcumin Improves Palmitate-Induced Insulin Resistance in Human Umbilical Vein Endothelial Cells by Maintaining Proteostasis in Endoplasmic Reticulum. Front Pharmacol. 8, 148 (2017)(PMID:28377722)
- Mitroi DN et al. SGPL1 (sphingosine phosphate lyase 1) modulates neuronal autophagy via phosphatidylethanolamine production. Autophagy 13, 885-899 (2017)(PMID:28521611)
- Tang Z et al. Atg2A/B deficiency switches cytoprotective autophagy to non-canonical caspase-8 activation and apoptosis. Cell Death Differ. 24, 2127-2138 (2017)(PMID:28800131)
- Taniguchi K et al. Depletion of gamma-glutamylcyclotransferase in cancer cells induces autophagy followed by cellular senescence. Am J Cancer Res. 8, 650-661 (2018)(PMID:29736310)
- Scrivo A et al. Gigaxonin E3 ligase governs ATG16L1 turnover to control autophagosome production. Nat Commun. 10, 780 (2019)(PMID:30770803)
Immunocytochemistry - Scrivo A et al. Gigaxonin E3 ligase governs ATG16L1 turnover to control autophagosome production. Nat Commun. 10, 780 (2019)(PMID:30770803)
|
Product category |
- Research area
- Autophagy
|