Code No. U-N001-10
WNT Surrogate-Fc Fusion Protein
価格（税別）
¥1,166,000
在庫
0
(お問合せ）
（2024/04/26 17:33時点）
包装
1 mg
データシートUse, storage and stability
Predilute the product to a 5 μM stock solution using a suitable buffer. The effective working concentration range varies between 0.1 - 0.5 nM and should be determined experimentally.
Undiluted Wnt Surrogate-Fc Fusion Protein should be stored at -80°C (stable for at least 1 year). The buffer contains 25 mM Tris 500 mM NaCl pH 8.2 without preservative. After thawing it should be stored in appropriate small aliquots at -20°C or -80°C (stable for at least 1 year).
Reconstituted Wnt Surrogate-Fc Fusion-protein in organoid culture medium is stable for 2 – 3 weeks at 4°C. Avoid repeated warm cycles to 37°C.
Production platform:
This product has been produced in HEK293 cells using the rPExTM recombinant protein production platform.
datasheet url: https://products.ipatherapeutics.com/product/n001-1-0mg/
Predilute the product to a 5 μM stock solution using a suitable buffer. The effective working concentration range varies between 0.1 - 0.5 nM and should be determined experimentally.
Undiluted Wnt Surrogate-Fc Fusion Protein should be stored at -80°C (stable for at least 1 year). The buffer contains 25 mM Tris 500 mM NaCl pH 8.2 without preservative. After thawing it should be stored in appropriate small aliquots at -20°C or -80°C (stable for at least 1 year).
Reconstituted Wnt Surrogate-Fc Fusion-protein in organoid culture medium is stable for 2 – 3 weeks at 4°C. Avoid repeated warm cycles to 37°C.
Production platform:
This product has been produced in HEK293 cells using the rPExTM recombinant protein production platform.
datasheet url: https://products.ipatherapeutics.com/product/n001-1-0mg/
| 使用目的 | stem cell culture; organoid | ||
|---|---|---|---|
| 保存温度 | -80°C | メーカー | ImmunoPrecise Antibodies (Europe) BV |
| 別称 | Surrogate Wnt agonist, Wnt | ||
| 背景 | Description The Wnt signaling pathway is essential for embryonic development, stem cell differentiation and regeneration of injured tissues. Wnts hetero-dimerize Frizzled (Fzd) family receptors and their co-receptors Lrp5/6, triggering downstream signaling pathways including the canonical β-catenin cascade, and resulting in expression of genes regulating cell development. Wnt has been widely used as an essential cell growth factor in organoids. Currently, recombinant Wnt3a is commonly used as Wnt source. Unfortunately, recombinant Wnt3a is water insoluble and cannot serve as a medium supplement. Recombinant Wnt Surrogate-Fc Fusion-protein has an excellent solubility, and a better wnt response at lower effective concentrations than other sources of Wnt3a. WNT Surrogate-Fc Fusion-protein supports long-term expansion of multiple different types of organoids, including intestinal, kidney, colon, hepatocyte, ovarian, and breast organoids. |
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| 備考 | オルガノイド等の培養において、本製品を他の因子との組合せで使用する場合、当該他の因子の使用等に第三者の特許が存在する可能性がございます。本製品は、他の因子との組合せによる使用等まで保証するものではございませんので、当該他の因子との組合せで使用する場合に関しては、ご所属の知財部・調査機関へご確認のうえ、本製品をご利用ください。 | ||
| 製品 カテゴリー |
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