トップ > 製品検索結果一覧 > Code No. PM097 Anti-NFIL3 (E4BP4) pAb

Code No. PM097

Anti-NFIL3 (E4BP4) pAb

価格(税別)

¥48,000

キャンペーン価格

¥33,600

(2020/03/31まで)

 > 概日リズム関連抗体キャンペーン2019

在庫

10以上

(2019/12/16 00:05時点)

包装

100 µL

データ
  • Western Blotting

    *Zeitgeber time: Mice were maintained in a light-dark (LD) cycle (12 hr light, 12 hr dark with lights on time was designatedas Zeitgeber time (ZT) 0 and light off time was designated as ZT12).

クローナリティー Polyclonal クローン Polyclonal
アイソタイプ
(免疫動物)
Rabbit Ig (aff.)
使用法
WB
1 µg/mL  
ChIP*
  (PMID: 31428688
交差性
[Gene ID]

Human[4783], Mouse[18030], Rat(-)

性状 1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度 -20°C 標識 Unlabeled メーカー MBL
別称 IL3BP1, NFIL3A, NF-IL3A
背景 NFIL3, or E4BP4, is a transcription factor that controls sleep and circadian rhythms throughout the cranial nerve, endocrine, and metabolic systems.The circadian clock is a 24-hour endogenous oscillator, which cycles via transcriptional/translational feedback loops of clock genes and their products in individual cells. CLOCK and BMAL1 are positive factors in the loop, and their complex binds to E-box elements of PERs and CRYs. PER and CRY bind the CLOCK-BMAL1 complex, suppressing E-box-dependent transactivation and forming a molecular clock via this negative feedback mechanism.D-box and REV-ERB/ROR-binding elements also form a regulatory network of clock-controlled genes (CCGs). D-box-dependent transactivation is activated by DBP and repressed by NFIL3/E4BP4.NFIL3/E4BP4 expression also plays an important role in the immune system. NFIL3 polymorphisms are associated with inflammatory bowel disease (IBD).
関連製品 PM096 Anti-PER2 (Human) pAb
M120-3 Anti-Foxp3 mAb
D335-3 Anti-BMAL1 mAb
CY-P1016 Anti-SIRT1 pAb
D334-3 Anti-CLOCK (Mouse) mAb
D333-3 Anti-CLOCK (Mouse) mAb
PM079 Anti-DBP (Mouse) pAb
PM075 Anti-GNAT2 (Zebrafish) pAb
PM081 Anti-Cry1 (Mouse) pAb
PM082 Anti-Cry2 (Mouse) pAb
PM083 Anti-Per2 (Mouse) pAb
PM080 Anti-RORγt pAb
PM087 Anti-Chrono (Mouse) pAb
PM091 Anti-Per1 (Mouse) pAb
PM092 Anti-NR1D1 (Rev-erbα) pAb
PM093 Anti-NR1D2 (Rev-erbβ) pAb
D349-3 Anti-CLOCK mAb
M219-3 Anti-RORγt mAb
M225-3 Anti-NFIL3 (E4BP4) chimeric mAb
CY-1173 CycLex® CaM-kinase II Assay Kit
使用文献
使用文献募集中!本製品を使って論文を発表されましたら是非お知らせください。

Western Blotting

  1. Imamura K et al. ASK family kinases mediate cellular stress and redox signaling to circadian clock. PNAS (2018) In press.(PMID:29555767)
  2. Yoshitane H et al. Functional D-box sequences reset the circadian clock and drive mRNA rhythms. Commun Biol. 2, 300 (2019)(PMID:31428688)

ChIP

  1. Yoshitane H et al. Functional D-box sequences reset the circadian clock and drive mRNA rhythms. Commun Biol. 2, 300 (2019)(PMID:31428688)
参考文献
  1. Yu X et al. Th17 cell differentiation is regulated by the circadian clock. Science 342, 727-30 (2013)(PMID: 24202171
  2. Firth MA et al. Nfil3-independent lineage maintenance and antiviral response of natural killer cells. J Exp Med. 210, 2981-90 (2013)
     (PMID: 24277151
  3. Kashiwada M et al. IL-4-induced transcription factor NFIL3/E4BP4 controls IgE class switching. PNAS 107, 821-6 (2010)(PMID: 20080759
  4. Mitsui S et al. Antagonistic role of E4BP4 and PAR proteins in the circadian oscillatory mechanism. Genes Dev. 15, 995-1006 (2001)(PMID: 11316793
  5. Ikushima S et al. Pivotal role for the NFIL3/E4BP4 transcription factor in interleukin 3-mediated survival of pro-B lymphocytes. PNAS 94, 2609-14 (1997)(PMID: 9122243
  6. Zhang W et al. Molecular cloning and characterization of NF-IL3A, a transcriptional activator of the human interleukin-3 promoter. Mol Cell Biol. 15, 6055–63 (1995)(PMID: 7565758
  7. Cowell IG et al. Transcriptional repression by a novel member of the bZIP family of transcription factors. Mol Cell Biol. 12, 3070-7 (1992)(PMID: 1620116
製品
カテゴリー
分野
免疫
概日リズム
この製品に関するお問い合わせをする

※在庫につきましては2019年12月16日 00時05分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について:
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
※使用法・交差性の表記について:
*: 論文で報告されております(MBLでは未確認)。詳しくはデータシートをご覧ください。
**: 導入元からの情報です(MBLでは評価中もしくは未確認)。
※保存温度の表記について: RT: 室温
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