トップ > 製品検索結果一覧 > Code No. M132-3 Anti-HA-tag mAb

Code No. M132-3

Anti-HA-tag mAb

価格(税別)

¥32,000

在庫

10以上

(2019/10/17 00:05時点)

包装

200 µg/100 µL

データ
  • Western Blotting

  • Immunoprecipitation

クローナリティー Monoclonal クローン 5D8
アイソタイプ
(免疫動物)
Mouse IgG1 κ
使用法
WB
1 µg/mL  
IP
1 µg/sample  
Co-IP*
reported.  (PMID: 20506502 / 21586271 / 27697867
免疫原(抗原) Carrier protein conjugated peptide (CP-YPYDVPDYA)
性状 2 mg/mL in PBS/50% glycerol, pH 7.2
保存温度 -20°C 標識 Unlabeled メーカー MBL
背景 Epitope tagging has widely been accepted technique that fuses an epitope peptide to a certain protein as a marker for gene expression. With this technique, the gene expression can be easily monitored on western blotting, immunoprecipitation and immunofluorescence utilizing with an antibody that recognizes such an epitope. Amino acid sequences that are widely used for the epitope tagging are as follow; YPYDVPDYA (HA-tag), EQKLISEEDL (Myc-tag) and YTDIEMNRLGK (VSV-G-tag), which corresponding to the partial peptide of Influenza hemagglutinin protein, Human c-myc gene product and Vesicular stomatitis virus glycoprotein respectively.
関連製品 M132-10 Anti-HA-tag mAb-Magnetic Agarose
M180-3 Anti-HA-tag mAb
M180-3S Anti-HA-tag mAb
M180-7 Anti-HA-tag mAb-HRP-DirecT
M180-10 Anti-HA-tag mAb-Magnetic Agarose
M180-A48 Anti-HA-tag mAb-Alexa Fluor® 488
M180-A59 Anti-HA-tag mAb-Alexa Fluor® 594
M180-A64 Anti-HA-tag mAb-Alexa Fluor® 647
561 Anti-HA-tag pAb
561-5 Anti-HA-tag pAb
561-7 Anti-HA-tag pAb-HRP-DirecT
561-8 Anti-HA-tag pAb-Agarose
561-A48 Anti-HA-tag pAb-Alexa Fluor® 488
M132-11 Anti-HA-tag mAb-Magnetic Beads
M180-11 Anti-HA-tag mAb-Magnetic Beads
使用文献
使用文献募集中!本製品を使って論文を発表されましたら是非お知らせください。

Western Blotting

  1. Ohshima T et al. HTLV‐1 basic leucine‐zipper factor, HBZ, interacts with MafB and suppresses transcription through a Maf recognition element. J Cell Biochem. 111, 187-94 (2010)(PMID:20506502)
  2. Kim TS et al. The ZFHX3 (ATBF1) transcription factor induces PDGFRB, which activates ATM in the cytoplasm to protect cerebellar neurons from oxidative stress. Dis Model Mech. 3, 752-62 (2010)(PMID:20876357)
  3. Mukai R, Ohshima T. Dual effects of HTLV-1 bZIP factor in suppression of interferon regulatory factor 1. Biochem Biophys Res Commun. 409, 328-32 (2011)(PMID:21586271)
  4. Mukai R, Ohshima T. HTLV-1 HBZ positively regulates the mTOR signaling pathway via inhibition of GADD34 activity in the cytoplasm. Oncogene. 33, 2317-28 (2014)(PMID:23708656)
  5. Zhang HH et al. Nemo-like kinase is critical for p53 stabilization and function in response to DNA damage. Cell Death Differ. 21, 1656-63 (2014)(PMID:24926618)
  6. Mukai R, Ohshima T. Enhanced Stabilization of MCL1 by the Human T-Cell Leukemia Virus Type 1 bZIP Factor Is Modulated by Blocking the Recruitment of Cullin 1 to the SCF Complex. Mol Cell Biol. 36, 3075-3085 (2016)(PMID:27697867)

Co-IP

  1. Ohshima T et al. HTLV‐1 basic leucine‐zipper factor, HBZ, interacts with MafB and suppresses transcription through a Maf recognition element. J Cell Biochem. 111, 187-94 (2010)(PMID:20506502)
  2. Mukai R, Ohshima T. Dual effects of HTLV-1 bZIP factor in suppression of interferon regulatory factor 1. Biochem Biophys Res Commun. 409, 328-32 (2011)(PMID:21586271)
  3. Mukai R, Ohshima T. Enhanced Stabilization of MCL1 by the Human T-Cell Leukemia Virus Type 1 bZIP Factor Is Modulated by Blocking the Recruitment of Cullin 1 to the SCF Complex. Mol Cell Biol. 36, 3075-3085 (2016)(PMID:27697867)

Immunoprecipitation

  1. Ogi T et al. Identification of the first ATRIP-deficient patient and novel mutations in ATR define a clinical spectrum for ATR-ATRIP Seckel Syndrome. PLoS Genet. 8, e1002945 (2012)(PMID:23144622)
  2. Mukai R, Ohshima T. HTLV-1 HBZ positively regulates the mTOR signaling pathway via inhibition of GADD34 activity in the cytoplasm. Oncogene. 33, 2317-28 (2014)(PMID:23708656)
  3. Zhang HH et al. Nemo-like kinase is critical for p53 stabilization and function in response to DNA damage. Cell Death Differ. 21, 1656-63 (2014)(PMID:24926618)
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※在庫につきましては2019年10月17日 00時05分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について:
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
※使用法・交差性の表記について:
*: 論文で報告されております(MBLでは未確認)。詳しくはデータシートをご覧ください。
**: 導入元からの情報です(MBLでは評価中もしくは未確認)。
※保存温度の表記について: RT: 室温
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