トップ > 製品検索結果一覧 > Code No. D221-3 Anti-Sap155 mAb

Code No.　D221-3

Anti-Sap155 mAb

価格（税別）

¥52,800

在庫

10以上

（2025/01/24 17:00時点）

包装

100 µL (1 mg/mL)

データ

  • Western Blotting

  • Immunoprecipitation

  • Immunocytochemistry
クローナリティー Monoclonal クローン 16
アイソタイプ
（免疫動物） 		Mouse IgG2b
使用法
WB
1 µg/mL  
IP
1 µg/200 µL of cell extract from 5x106 cells  
IC
10 µg/mL  
免疫原(抗原) Recombinant Sap155 (98-198 a.a.)
交差性
[Gene ID]

Human[23451](PMID: 18842582), Mouse[81898]
性状 1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度 -20°C 標識 Unlabeled メーカー MBL
別称 SF3B1, MDS, PRP10, Hsh155, PRPF10, SF3b155/TA-8, Prp10, Targ4, SAP155, SF3b155
背景 SF3 is a U2 snRNP-associated protein complex essential for spliceosome assembly and splicing catalysis of the major spliceosome. SF3 contains the Spliceosome-Associated Proteins, Sap 49, 130, 145, and 155. Sap155/Sf3b1 is an essential subunit of the U2 snRNP for mRNA splicing and has also been identified in the minor (U12-dependent) spliceosome. Sap155 interacts with the mammalian PcG (Polycomb group) proteins, Mel18 and Ring1B by the yeast two-hybrid system. Sap155 contains numerous Cdk consensus phosphorylation sites in its N-terminus and is phosphorylated prior to catalytic step II of the splicing pathway. Sap155 serves as a substrate for cyclin E-cdk2 in vitro, suggesting that pre-mRNA splicing may be linked to the cell cycle machinery in mammalian cells.
関連製品 D138-3 Anti-Sap155 mAb
D139-3 Anti-Ring1B mAb
RN085PW Anti-U2AF1 pAb
使用文献
 使用文献募集中！本製品を使って論文を発表されましたら是非お知らせください。

Western Blotting

  1. Kotake Y et al. Splicing factor SF3b as a target of the antitumor natural product pladienolide. Nat Chem Biol. 3, 570-5 (2007)(PMID:17643112)
  2. Yamasaki S et al. Mincle is an ITAM-coupled activating receptor that senses damaged cells. Nat Immunol. 9, 1179-1188 (2008)(PMID:18776906)
  3. Tanuma N et al. Nuclear inhibitor of protein phosphatase-1 (NIPP1) directs protein phosphatase-1 (PP1) to dephosphorylate the U2 small nuclear ribonucleoprotein particle (snRNP) component, spliceosome-associated protein 155 (Sap155). J Biol Chem. 283, 35805-14 (2008)(PMID:18842582)
  4. Yokoi A et al. Biological validation that SF3b is a target of the antitumor macrolide pladienolide. FEBS J. 278, 4870-80 (2011)(PMID:21981285)
  5. Takemura R et al. Multiple factors in the early splicing complex are involved in the nuclear retention of pre-mRNAs in mammalian cells. Genes Cells. 16, 1035-49 (2011)(PMID:21929696)
  6. Manceau V et al. The protein kinase KIS impacts gene expression during development and fear conditioning in adult mice. PLoS One 7, e43946 (2012)(PMID:22937132)
  7. Kajiwara T et al. SAP155-mediated c-myc suppressor far-upstream element-binding protein-interacting repressor splicing variants are activated in colon cancer tissues. Cancer Sci.104, 149-56 (2013)(PMID:23113893)
  8. Gao Y et al. Regulation of HPV16 E6 and MCL1 by SF3B1 inhibitor in head and neck cancer cells. Sci Rep. 4, 6098 (2014)(PMID:25139387)
  9. Kfir N et al. SF3B1 association with chromatin determines splicing outcomes. Cell Rep. 11, 618-29 (2015)(PMID:25892229)
  10. Schreiber CA et al. An siRNA Screen Identifies the U2 snRNP Spliceosome as a Host Restriction Factor for Recombinant Adeno-associated Viruses. PLoS Pathog. 11, e1005082 (2015)(PMID:26244496)
  11. Lee SC et al. Modulation of splicing catalysis for therapeutic targeting of leukemia with mutations in genes encoding spliceosomal proteins. Nat Med. 22, 672-8 (2016)(PMID:27135740)
  12. Cossa G et al. Localized Inhibition of Protein Phosphatase 1 by NUAK1 Promotes Spliceosome Activity and Reveals a MYC-Sensitive Feedback Control of Transcription. Mol Cell. 77, 1322-1339.e11 (2019)(PMID:32006464)

Immunoprecipitation

  1. Kotake Y et al. Splicing factor SF3b as a target of the antitumor natural product pladienolide. Nat Chem Biol. 3, 570-5 (2007)(PMID:17643112)
  2. Tanuma N et al. Nuclear inhibitor of protein phosphatase-1 (NIPP1) directs protein phosphatase-1 (PP1) to dephosphorylate the U2 small nuclear ribonucleoprotein particle (snRNP) component, spliceosome-associated protein 155 (Sap155). J Biol Chem. 283, 35805-14 (2008)(PMID:18842582)
  3. Kfir N et al. SF3B1 association with chromatin determines splicing outcomes. Cell Rep. 11, 618-29 (2015)(PMID:25892229)

Immunohistochemistry

  1. Sciarrillo R et al. Splicing modulation as novel therapeutic strategy against diffuse malignant peritoneal mesothelioma. EBioMedicine 39, 215-225 (2019)(PMID:30581150)
製品
カテゴリー
分野
RNA-RNPネットワーク
エピジェネティクス
がん
この製品に関するお問い合わせをする

※在庫につきましては2025年01月24日 17時00分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
※使用法・交差性の表記について：
*: 論文で報告されております（MBLでは未確認）。詳しくはデータシートをご覧ください。
**: 導入元からの情報です（MBLでは評価中もしくは未確認）。
※保存温度の表記について： RT: 室温
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