トップ > 製品検索結果一覧 > Code No. D139-3 Anti-Ring1B mAb

Code No.　D139-3

Anti-Ring1B mAb

価格（税別）

¥52,800

在庫

10以上

（2025/01/24 17:00時点）

包装

100 µL (1 mg/mL)

データ

  • Immunoprecipitation
クローナリティー Monoclonal クローン 3-3
アイソタイプ
（免疫動物） 		Mouse IgG2b
使用法
WB
1 µg/mL  
IP
1-5 µg/200-300 µL of cell extract  
IH*
reported.  （PMID: 20351251）
IF*
reported.  （PMID: 24055057）
ChIP*
reported.  （PMID: 20473294 / 21170310）
免疫原(抗原) Recombinant Mouse full length Ring1B
交差性
[Gene ID]

Human[6045], Mouse[19821], Hamster
性状 1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度 -20°C 標識 Unlabeled メーカー MBL
別称 Rnf2/ring finger protein 2, BAP1, DING, BAP-1, HIPI3, RING1B/dinG
背景 Polycomb-group (PcG) proteins form multimeric complexes that maintain the state of transcriptional repression of several regulatory genes during development. Ring1B/Rnf2 forms part of a protein complex containing other PcG proteins, such as Mel18, Bmi1, MBLR, MPc3, and the spliceosome protein Sap155, and these complexes associate with chromatin to regulate transcription. Ring1B may also play a role in the regulation of Hox gene expression by PcG complexes. Deletion of Ring1B activity results in gastrulation arrest and cell cycle inhibition.
関連製品 D138-3 Anti-Sap155 mAb
D221-3 Anti-Sap155 mAb
D210-3 Anti-Histone H2A mAb
使用文献
 使用文献募集中！本製品を使って論文を発表されましたら是非お知らせください。

Western Blotting

  1. Sarcinella E et al. Monoubiquitylation of H2A.Z distinguishes its association with euchromatin or facultative heterochromatin. Mol Cell Biol. 27, 6457-68 (2007)(PMID:17636032)
  2. Wu X et al. Cooperation between EZH2, NSPc1-mediated histone H2A ubiquitination and Dnmt1 in HOX gene silencing. Nucleic Acid Res. 36, 3590-9 (2008)(PMID:18460542)
  3. Wu J et al. Histone ubiquitination associates with BRCA1-dependent DNA damage response. Mol Cell Biol. 29, 849-60 (2009)(PMID:19015238)
  4. Alchanati I et al. The E3 ubiquitin-ligase Bmi1/Ring1A controls the proteasomal degradation of Top2alpha cleavage complex - a potentially new drug target. PLoS One 4, e8104 (2009)(PMID:19337376)
  5. Zaaroor-Regev D et al. Regulation of the polycomb protein Ring1B by self-ubiquitination or by E6-AP may have implications to the pathogenesis of Angelman syndrome. PNAS 107, 6788-93 (2010)(PMID:20351251)
  6. Pradeepa MM et al. Psip1/Ledgf p75 restrains Hox gene expression by recruiting both trithorax and polycomb group proteins. Nucleic Acids Res. 42, 9021-32 (2014)(PMID:25056311)
  7. Tatavosian R et al. Distinct Cellular Assembly Stoichiometry of Polycomb Complexes on Chromatin Revealed by Single-molecule Chromatin Immunoprecipitation Imaging. J Biol Chem. 290, 28038-54 (2015)(PMID:26381410)
  8. Arora M et al. RING1A and BMI1 bookmark active genes via ubiquitination of chromatin-associated proteins. Nucleic Acids Res. 44, 2136-44 (2016)(PMID:26578590)

ChIP

  1. Landeira D et al. Jarid2 is a PRC2 component in embryonic stem cells required for multi-lineage differentiation and recruitment of PRC1 and RNA Polymerase II to developmental regulators. Nat Cell Biol. 12, 618-24 (2010)(PMID:20473294)
  2. Mendenhall EM et al. GC-rich sequence elements recruit PRC2 in mammalian ES cells. PLoS Genet. 6, e1001244 (2010)(PMID:21170310)
  3. Wu X et al. Cooperation between EZH2, NSPc1-mediated histone H2A ubiquitination and Dnmt1 in HOX gene silencing. Nucleic Acid Res. 36, 3590-9 (2008)(PMID:18460542)
  4. Barco R et al. The synovial sarcoma-associated SYT-SSX2 oncogene antagonizes the polycomb complex protein Bmi1. PLoS One 4, e5060 (2009)(PMID:19337376)
  5. Williamson I et al. Anterior-posterior differences in HoxD chromatin topology in limb development. Development 139, 3157-67 (2012)(PMID:22872084)
  6. Pradeepa MM et al. Psip1/Ledgf p75 restrains Hox gene expression by recruiting both trithorax and polycomb group proteins. Nucleic Acids Res. 42, 9021-32 (2014)(PMID:25056311)
  7. Vallot C et al. PRC2-independent chromatin compaction and transcriptional repression in cancer. Oncogene 34, 741-51 (2015)(PMID:24469045)
  8. Gao R et al. Bmi1 promotes erythroid development through regulating ribosome biogenesis. Stem Cells. 33, 925-38 (2015)(PMID:25385494)
  9. Tatavosian R et al. Distinct Cellular Assembly Stoichiometry of Polycomb Complexes on Chromatin Revealed by Single-molecule Chromatin Immunoprecipitation Imaging. J Biol Chem. 290, 28038-54 (2015)(PMID:26381410)

Immunoprecipitation

  1. Barco R et al. The synovial sarcoma-associated SYT-SSX2 oncogene antagonizes the polycomb complex protein Bmi1. PLoS One 4, e5060 (2009)(PMID:19337376)
  2. Zaaroor-Regev D et al. Regulation of the polycomb protein Ring1B by self-ubiquitination or by E6-AP may have implications to the pathogenesis of Angelman syndrome. PNAS 107, 6788-93 (2010)(PMID:20351251)
  3. Si S et al. Loss of Pcgf5 Affects Global H2A Monoubiquitination but Not the Function of Hematopoietic Stem and Progenitor Cells. PLoS One. 11, e0154561 (2016)(PMID:27136092)

Immunohistochemistry

  1. Zaaroor-Regev D et al. Regulation of the polycomb protein Ring1B by self-ubiquitination or by E6-AP may have implications to the pathogenesis of Angelman syndrome. PNAS 107, 6788-93 (2010)(PMID:20351251)

Immunofluorescence

  1. Casanova M et al. Heterochromatin reorganization during early mouse development requires a single-stranded noncoding transcript. Cell Rep. 4, 1156-67 (2013)(PMID:24055057)
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※在庫につきましては2025年01月24日 17時00分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
※使用法・交差性の表記について：
*: 論文で報告されております（MBLでは未確認）。詳しくはデータシートをご覧ください。
**: 導入元からの情報です（MBLでは評価中もしくは未確認）。
※保存温度の表記について： RT: 室温
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