Both mini-AID-tag and full-length AID-tag can be detected.
Auxin-inducible degron (AID) technology enables to deplete a protein of interest in a half-life less than 30 min by the addition of auxin to culture medium1). Auxin includes chemicals such as IAA (indole-3-aceticacid) and NAA (1-naphtalene acetic acid) and includes inhibitors in plants. For the degradation, and SCF ubiquitin ligase complex containing the TIR1 protein is activated via the association of auxin with TIR1. Because the core SCF components are conserved in all eukaryotic cells, it is possible to transplant the degradation pathway to yeast and mammalian cells by expressing TIR1. In those cells expressing TIR1, a protein fused with degron (AID degron) derived from AUX/IAA can be rapidly degraded in the presence of auxin.
mini-AID-tag (8kDa) was developed based on the full-length AID-tag (25kDa)2）. Our original anti-mini-AID-tag, Clone 1E4, can detect mini-AID-tag and full-length AID-tag as well.
*Expression vector can be obtained by RikenBRC, NBRP-Yeast, or Addgene.
*The piicture on the right was provided by Dr. Masato Kanemaki from NIG and edited by MBL.
Anti-mini-AID-tag mAb data
■ Western Blotting (M214-3)
Can detect full-length of AID-tag and mini-AID-tag.
■ Western Blotting (M214-7)
Code M214-7 is HRP directly labeled product. No need of secondary antibodies.
The performance has been proven in articles.
Natsume T et al. Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors.
Cell Rep. 15, 210-8 (2016)(PMID:27052166)
Kubota T et al. The Elg1 Replication Factor C-like Complex Functions in PCNA Unloading during DNA Replication. Molecular Cell, 50, 273-280 （2013） （PMID:23499004）
Nishimura K and Kanemaki MT. Rapid Depletion of Budding Yeast Proteins via the Fusion of an Auxin-Inducible Degron (AID). Current Protocols in Cell Biology, 64, 20.9.1-20.9.16 (2014) (PMID:25181302)