|30min for Western Blotting!|
|Can get high sensitivity and save antibody!|
|No cross-reactivity with antibodies used for immunoprecipitation eliminating their heavy and light chain bands in the blots.|
"HRP-DirecT series" is a series of epitope tag antibodies labeled directlyy with HRP.
MBL has succeeded to develop the HRP labeled antibodies with higher sensitivity and lower background.
ProteinA/G blocking reagent for HRP-DirecT
Boiling the Immunoprecipitated sample with Laemmli’s sample buffer can causes dissociation of protein A/G from its carrier. Therefore, the primary and secondary antibodies bind to protein A/G at western blotting. Add Clear Back for IP Western to HRP-DirecT will sort this problem.