Tag-protein Purification Kit & Gel

To purify target epitope-tagged proteins without any loss of protein activity and denature, all procedure during purification should be conducted under a neutral pH condition. Severe conditions such as acidic or alkaline elution can occasionally alter protein structures or biological functions of target proteins.

MBL's tagged Protein PURIFICATION KIT employs competitive polypeptides to elute target tagged proteins under a neutral pH for maintaining target protein function and conformation. Epitope tag specific antibodies in this kit are highly optimized for target recovery with high yield and purity from crude samples like cell lysates or culture supernatant of mammal trandfectant cells.

*This products is a set of purification gel, elution peptide, wash buffer and mini column.

Procedure Summary

Purification and enzymatic activity of N-terminal His-tagged β-galactosidase
His tagged Protein PURIFICATION KIT (Code No. 3310)

Pull-down assay
HA tagged Protein PURIFICATION KIT (Code No. 3320)

Purification of C-terminal his-tagged protein X from culture supernatant
His tagged Protein PURIFICATION KIT (Code No. 3310)

*This product is a set of purification gel and elution peptide.

Purification of C-terminal His-tagged protein X from culture supernatant
His tagged Protein PURIFICATION GEL with Elution Peptide (Code No. 3311)

SDS-PAGE (Coomassie Brilliant Blue Staining)
Stable transfectant of CHO (Chinese Hamster Ovary) cells expressing C-terminal His-tagged protein were cultured for 7 days in DMEM medium containing 10% Fetal bovine serum. C-terminal His-tagged protein was purified from 50 mL of cultured medium according to the preceding protocol. Column bed volume was 0.5 mL. Elution was carried out with 2.5 mL of 1 mg/mL His tag peptide. Wach fraction was 0.5 mL.

*This product consists of a purification gel only.

Purification of N-terminal DDDDK-tagged β-Galactosidase
DDDDK-tagged Protein PURIFICATION GEL (Code No. 3328)

SDS-PAGE (Coomassie Brilliant Blue Staining)
Human embryonic kidney cell (293T) were transfected with pcDNA-DDDDK-tagged β-galactosidase and cultured for 60 hours. Cells were then lysed in the Lysis buffer (10 mL/100-mm dishes x5) and purified according to the preceding protocol. Column bed volume was 0.25 mL. Elution was carried out with 2 mL of 0.1 mg/mL DDDDK-tag peptide.
Each fraction was 0.25 mL.

Ready-to-use-cartridge for the isolation of tagged protein.

＊ Maximum working pressure: 0.3 MPa
ÄKTA is a trade mark of GE Healthcare (Life Sciences).

Example of Purification Result
c-Myc-tagged Protein PURIFICATION CARTRIDGE (Code No. 3306K)

MBL offers small packages of purification kits for c-Myc-, V5-, HA-, and DDDDK-tagged proteins at a low cost. In many research fields, purification of proteins in their active forms or native conformations is essential. To purify proteins while preserving their activity and structure, purification is best performed under mild, neutral conditions rather than harsh conditions, such as acid or alkaline.

The Tagged Protein Magnetic PURIFICATION KITs contain magnetic beads coated with an antibody with high specificity and optimal affinity for each tag. These Kits enable high-purity tagged proteins expressed in mammalian cells to be isolated under mild, neutral conditions in a simple procedure.

*This product is a set of magnetic conjugated antibodies, elution peptide and wash solution.

Procedure Summary

c-Myc-tagged GST protein purification
c-Myc-tagged Protein Magnetic Purification kit (Code No. 3340)

V5-tagged β-galactosidase purification
V5-tagged Protein Magnetic Purification Kit (Code No. 3341)

HA-tagged β-galactosidase purification
HA-tagged Protein Magnetic Purification Kit（Code No. 3342）

DDDDK-tagged β-galactosidase purification
DDDDK-tagged Protein Magnetic Purification Kit（Code No. 3343R）