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Tag-protein Purification Kit & Gel



Product line-up

 PURIFICATION KIT

 

To purify target epitope-tagged proteins without any loss of protein activity and denature, all procedure during purification should be conducted under a neutral pH condition. Severe conditions such as acidic or alkaline elution can occasionally alter protein structures or biological functions of target proteins.

MBL's tagged Protein PURIFICATION KIT employs competitive polypeptides to elute target tagged proteins under a neutral pH for maintaining target protein function and conformation. Epitope tag specific antibodies in this kit are highly optimized for target recovery with high yield and purity from crude samples like cell lysates or culture supernatant of mammal trandfectant cells.

Tagged Protein PURIFICATION KIT *This products is a set of purification gel, elution peptide, wash buffer and mini column.

  • Recommmended to who wants to purify from the small samples.
  • Almost everything is in this kit to start. Easy to get ready!
  • Perfect for pull-down assay.


Procedure Summary

Tagged protein purification kit procedure summary



purified in native conformations
 Our purification procedure under a neutral pH condition enables maintaining target protein
 function and conformation.
 Useful/available for pull-down assay as well to analyze co-immunoprecipitated proteins.

Purification and enzymatic activity of N-terminal His-tagged β-galactosidase
His tagged Protein PURIFICATION KIT (Code No. 3310)

3310 Purification and enzymatic activity of N-terminal His-tagged β-galactosidase

Pull-down assay
HA tagged Protein PURIFICATION KIT (Code No. 3320)

3320 pull-down assay



High purity Able to recover high purity target tagged proteins by using our original proprietary antibodies that have superior affinity and specificity to the epitope tag.

Purification of C-terminal his-tagged protein X from culture supernatant
His tagged Protein PURIFICATION KIT (Code No. 3310)

3310 purification from supernatant with serum




 PURIFICATION GEL with Elution Peptide

 

Tagged Protein PURIFICATION GEL with Elution Peptide *This product is a set of purification gel and elution peptide.

  • Recommended for the large scale of protein purification.
  • Gel and peptide are sold separetly.


Purification of C-terminal His-tagged protein X from culture supernatant
His tagged Protein PURIFICATION GEL with Elution Peptide (Code No. 3311)

3311 Purification of C-terminal His-tagged protein X from culture supernatant
SDS-PAGE (Coomassie Brilliant Blue Staining)
Stable transfectant of CHO (Chinese Hamster Ovary) cells expressing C-terminal His-tagged protein were cultured for 7 days in DMEM medium containing 10% Fetal bovine serum. C-terminal His-tagged protein was purified from 50 mL of cultured medium according to the preceding protocol. Column bed volume was 0.5 mL. Elution was carried out with 2.5 mL of 1 mg/mL His tag peptide. Wach fraction was 0.5 mL.





 PURIFICATION GEL

 

Tagged Protein PURIFICATION GEL *This product consists of a purification gel only.

  • Recommended for the large scale of protein purification.
  • For who wants to prepare elution peptide by temselves.



Purification of N-terminal DDDDK-tagged β-Galactosidase
DDDDK-tagged Protein PURIFICATION GEL (Code No. 3328)

3328 Purification of N-terminal DDDDK-tagged β-GalactosidaseSDS-PAGE (Coomassie Brilliant Blue Staining)
Human embryonic kidney cell (293T) were transfected with pcDNA-DDDDK-tagged β-galactosidase and cultured for 60 hours. Cells were then lysed in the Lysis buffer (10 mL/100-mm dishes x5) and purified according to the preceding protocol. Column bed volume was 0.25 mL. Elution was carried out with 2 mL of 0.1 mg/mL DDDDK-tag peptide.
Each fraction was 0.25 mL.




 PURIFICATION CARTRIDGE

 

Tagged Protein PURIFICATION CARTRIDGE Ready-to-use-cartridge for the isolation of tagged protein.

  • The cartrige can be used directly on automated ÄKTA or FPLC systems. (Cartridge i 1/16 inch female end)

* Maximum working pressure: 0.3 MPa
ÄKTA is a trade mark of GE Healthcare (Life Sciences).

Example of Purification Result
c-Myc-tagged Protein PURIFICATION CARTRIDGE (Code No. 3306K)

3306K Example of purification result



 TAGGED PROTEIN MAGNETIC PURIFICATION KIT

 

MBL offers small packages of purification kits for c-Myc-, V5-, HA-, and DDDDK-tagged proteins at a low cost. In many research fields, purification of proteins in their active forms or native conformations is essential. To purify proteins while preserving their activity and structure, purification is best performed under mild, neutral conditions rather than harsh conditions, such as acid or alkaline.

The Tagged Protein Magnetic PURIFICATION KITs contain magnetic beads coated with an antibody with high specificity and optimal affinity for each tag. These Kits enable high-purity tagged proteins expressed in mammalian cells to be isolated under mild, neutral conditions in a simple procedure.

Tagged Protein Magnetic PURIFICATION KIT*This product is a set of magnetic conjugated antibodies, elution peptide and wash solution.

  • Recommended for purifying small amount samples.
  • Set of needed reagents.
  • No need to centrifuge.
  • High yield.
  • Maintaining target protein function and conformation.


Procedure Summary

Tagged protein magnetic purification kit procedure summary.png



c-Myc-tagged GST protein purification
c-Myc-tagged Protein Magnetic Purification kit (Code No. 3340)

3340 c-Myc-tagged GST protein purification

V5-tagged β-galactosidase purification
V5-tagged Protein Magnetic Purification Kit (Code No. 3341)

3341 V5-tagged β-galactosidase purification

HA-tagged β-galactosidase purification
HA-tagged Protein Magnetic Purification Kit(Code No. 3342)

3342 HA-tagged β-galactosidase purification

DDDDK-tagged β-galactosidase purification
DDDDK-tagged Protein Magnetic Purification Kit(Code No. 3343)

3343 DDDDK-tagged β-galactosidase purification