Data |
Immunoprecipitation
|
Clonality |
Polyclonal
|
Clone |
Polyclonal |
Isotype
(Immunized Animal)
|
Rabbit IgG
|
Applications |
- WB
- 1:1,000
- IP
- 5 µL/sample
- IC
- 1:200
- IH
- 1:100 (Heat treatment is necessary for paraffin embedded sections.)
|
Immunogen
(Antigen) |
Recombinant full length luciferase (photinus pyralis)
|
Reactivity
[Gene ID] |
Other |
Storage buffer |
PBS/50% glycerol, pH 7.2
|
Storage temp. |
-20°C
|
Conjugate |
Unlabeled |
Manufacturer |
MBL |
Background |
Luciferase is a 61 kDa luminescent enzyme isolated from Photinus pyralis (North American Firefly). The enzyme catalyzes the ATP dependent oxidation of beetle luciferin to produce inorganic phosphate and light. Luciferase is frequently used as a reporter protein to quantify expression levels from promoters in tissue culture and in vivo. |
Related products |
M095-3 Anti-Luciferase mAb PM047 Anti-Renilla Luciferase pAb
|
Notes |
This antibody does not cross-react with the luciferase derived from Renilla mullerei.
|
Citations |
- Whitney AK, Campbell EL. Imaging Inflammatory Hypoxia in the Murine Gut. Methods Mol Biol. 1422, 115-26 (2016)(PMID:27246027)
Western Blotting - Ali Z et al. Dicistronic binary vector system-A versatile tool for gene expression studies in cell cultures and plants. J Biotechnol. 145, 9-16 (2010)(PMID:19835918)
- Itakura E et al. Heparan sulfate is a clearance receptor for aberrant extracellular proteins. J Cell Biol. 219, e201911126 (2020)(PMID:32211892)
Immunohistochemistry - Carruthers KH et al.Fat grafting as a vehicle for the delivery of recombinant adenoassociated viral vectors to achieve gene modification of muscle flaps. Ann Plast Surg. 70, 726-31 (2013)(PMID:23403543)
- Weger M et al. Real-time in vivo monitoring of circadian E-box enhancer activity: A robust and sensitive zebrafish reporter line for developmental, chemical and neural biology of the circadian clock. Dev Biol. 380, 259-73 (2013)(PMID:23665472)
- Sawada Y et al. GPRC5A facilitates cell proliferation through cell cycle regulation and correlates with bone metastasis in prostate cancer. Int J Cancer. 10, 1002 (2019)(PMID:31276604)
|
Product category |
- Tools
- Epitope tags
|