Antibody detailed product information

Code No. PM016
Anti-Luciferase pAb
Price ¥41,800
Size 100 µL
Availability (in Japan) 10 or more
（In Japan at 17:00, Apr 25, 2024 in JST）
Clonality Polyclonal
Clone Polyclonal
Isotype
(Immunized Animal) 		Rabbit IgG
Applications WB　1:1,000  
IP　5 µL/sample  
IC　1:200  
IH　1:100 (Heat treatment is necessary for paraffin embedded sections.)  
Immunogen
(Antigen)		 Recombinant full length luciferase (photinus pyralis)
Reactivity [Gene ID]

Other
Storage buffer PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Background Luciferase is a 61 kDa luminescent enzyme isolated from Photinus pyralis (North American Firefly). The enzyme catalyzes the ATP dependent oxidation of beetle luciferin to produce inorganic phosphate and light. Luciferase is frequently used as a reporter protein to quantify expression levels from promoters in tissue culture and in vivo.
Related products M095-3 Anti-Luciferase mAb
PM047 Anti-Renilla Luciferase pAb
Notes This antibody does not cross-react with the luciferase derived from Renilla mullerei.
Product category Tools:Epitope tags
Data

Citations
  1. Whitney AK, Campbell EL. Imaging Inflammatory Hypoxia in the Murine Gut. Methods Mol Biol. 1422, 115-26 (2016)(PMID:27246027)

Western Blotting

  1. Ali Z et al. Dicistronic binary vector system-A versatile tool for gene expression studies in cell cultures and plants. J Biotechnol. 145, 9-16 (2010)(PMID:19835918)
  2. Itakura E et al. Heparan sulfate is a clearance receptor for aberrant extracellular proteins. J Cell Biol. 219, e201911126 (2020)(PMID:32211892)

Immunohistochemistry

  1. Carruthers KH et al.Fat grafting as a vehicle for the delivery of recombinant adenoassociated viral vectors to achieve gene modification of muscle flaps. Ann Plast Surg. 70, 726-31 (2013)(PMID:23403543)
  2. Weger M et al. Real-time in vivo monitoring of circadian E-box enhancer activity: A robust and sensitive zebrafish reporter line for developmental, chemical and neural biology of the circadian clock. Dev Biol. 380, 259-73 (2013)(PMID:23665472)
  3. Sawada Y et al. GPRC5A facilitates cell proliferation through cell cycle regulation and correlates with bone metastasis in prostate cancer. Int J Cancer. 10, 1002 (2019)(PMID:31276604)
  • The availability is based on the information in Japan at 17:00, Apr 25, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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