Anti-Rubicon (Human) pAb

Price

¥52,800

Availability (in Japan)

10 or more

（In Japan at 00:05,
Apr 19, 2024 in JST）

Size

100 µL

Data	Western Blotting Western Blotting Immunoprecipitation Immunohistochemistry
Clonality	Polyclonal	Clone	Polyclonal
Isotype (Immunized Animal)	Rabbit Ig (aff.)
Applications	WB 1:1,000 IP 5 µL/300 µL of cell extract from 3 x 10⁶ cells IH Can be used.
Immunogen (Antigen)	Recombinant human Rubicon, C-terminal (722-972 aa)
Reactivity [Gene ID]	Human[9711], Mouse(-)
Storage buffer	PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	KIAA0226
Background	Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. Rubicon was identified as Beclin1 interacting protein. Three distinct Beclin1 complexes exist in cells, one of the complexes including Rubicon (Beclin1, hVps34, hVps15, UVRAG, Rubicon) down regulates the process of autophagosome maturation and endocytosis.
Related products	M170-3 Anti-Rubicon (Human) mAb PD017 Anti-Beclin 1 pAb M160-3 Anti-UVRAG mAb PD026 Anti-Atg14 pAb M184-3 Anti-Atg14 (Human) mAb PM072 Anti-VMP1 pAb MD-06-3 Anti-PI3-kinase (p85α) mAb
Citations	Western Blotting Maejima Y et al. Mst1 inhibits autophagy by promoting the interaction between Beclin1 and Bcl-2. Nat Med.19, 1478-88 (2013)(PMID:24141421) Bejarano E et al. Connexins modulate autophagosome biogenesis. Nat Cell Biol. 16, 401-14 (2014)(PMID:24705551)
Product category	Research area Autophagy

The availability is based on the information in Japan at 00:05, Apr 19, 2024 in JST.
The special price is shown in red color.
Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.