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HOME > Product search results > Code No. MT-20-3 Anti-DNA Polymerase α mAb

Code No. MT-20-3

Anti-DNA Polymerase α mAb

Price

¥48,000

Availability (in Japan)

10 or more

(In Japan at 00:05,
Jan 20, 2020 in JST)

Size

100 µg/100 µL

Data
  • Flow Cytometry

  • Immunocytochemistry

Clonality Monoclonal Clone CL22-2-42B
Isotype (Immunized Animal) Mouse IgG1
Applications
FCM
2 µg/mL (final concentration)  
IC
2 µg/mL  
IH
1-2.5 μg/mL (for cryosection)  
Immunogen (Antigen) purified DNA polymerase α from calf thymus
Reactivity [Gene ID]

Human[5422], Rat(-)

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names POLA1
Background DNA polymerases are essential enzymes involved in DNA replication and in repair. The
human genome contains 16 distinct known DNA polymerase genes, and these are classified into four families A, B, X, and Y based on their amino acid sequences. DNA polymerases α (pol α) belong to class B DNA polymerases. The pol α is the only known DNA polymerase able to initiate de novo DNA synthesis and is required for both initiation at chromosomal origins and for the synthesis of Okasaki fragments on the lagging strand. The pol α is composed of four subunits (p180, p68, p58, and p48) with distinctive functions. The polymerase activity is associated with the largest (p180) subunit, whereas the primase activity is associated with the p48 subunit. The p58 subunit contains a nuclear localization signal that is capable of directing both the p58 monomer and the p48-p58 dimer to the nucleus. The p68 subunit
binds tightly to the p180 subunit. The p68 subunit is essential for initial DNA synthesis, and is phosphorylated and dephosphorylated in a cell cycle-dependent manner.
Related products M075-3 Mouse IgG1 (isotype control)
Citations

Immunohistochemistry

  1. Yamaguchi A et al. Detection of the growth fraction in colorectal tumours by a monoclonal antibody against DNA polymerase alpha. Br J Cancer 61,390-3 (1990)(PMID:2328204)
  2. Yamaguchi A et al. DNA polymerase alpha positive-cell rate in colorectal cancer and its relationship to prognosis. Br J Cancer 65, 421-4 (1992)(PMID:1558798)
  3. Lattanzi G et al. Ultrastructural aspects of the DNA polymerase alpha distribution during the cell cycle. J Histochem Cytochem. 46, 1435-42 (1998)(PMID:9815285)
Product category
Cancer
Research area
Cancer
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  • The availability is based on the information in Japan at 00:05, Jan 20, 2020 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.